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- Clone
- W21036I (See other available formats)
- Regulatory Status
- RUO
- Other Names
- B7 homolog 2 (B7H2), GL50, B7 related protein 1 (B7RP1), CD275, inducible T cell costimulator ligand ICOS Ligand), LICOS, ICOSLG
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Biotinylated Recombinant Human B7-H2 (ICOSL)-Fc chimera (Cat. No. 798704) (black circles) binds to immobilized human ICOS-Fc chimera (Cat. No. 599004). Ultra-LEAF™ purified anti-human CD275 (B7-H2, ICOSL) (clone W21036I) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2a, κ isotype control (Cat. No. 400544) (gray triangles) does not have this effect. ND50 range: 0.01 - 0.07 µg/mL.
| Cat # | Size | Price | Save |
|---|---|---|---|
| 387505 | 100 µg | ¥69,720 | |
| 387506 | 1 mg | ¥202,520 |
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD275 (B7-H2, ICOSL) belongs to the B7 family of immunoregulatory molecules and is a transmembrane glycoprotein of approximately 60 kD. Mature human CD275 consists of 238 amino acid extracellular domain (ECD), two immunoglobulin-like domains, a 21 amino acid transmembrane segment, and a 25-residue cytoplasmic domain. Within the ECD, human CD275 shares 50% and 54% amino acid sequence homology with mouse and rat CD275, respectively. CD275 is expressed on antigen presenting cells such as B cells, macrophages, dendritic cells, and in monocytes. It binds to ICOS and is expressed on activated T cells, which leads to both positive and negative effects on immune responses including its own downregulation. Mouse and human CD275 exhibit cross species binding to ICOS. CD275 interaction with ICOS is costimulatory for T cell proliferation as well as the development of B cells, plasma cells, follicular helper T cells, and germinal centers. In addition, human CD275 binds to CD28 and CTLA4. The interaction of human CD275 and CD28 can co-stimulate both human mouse naïve T cells and regulatory T cells (Treg). CD275 plays a role in the development of allergic asthma by enhancing Th2 biased immune responses, limiting Th17 responses, and promoting eosinophilic infiltration into the lungs. CD275 activation by ICOS on Tregs limits pulmonary inflammation and airway hyper-responsiveness, promotes the development of inhalational tolerance, and impairs anti-tumor immunity.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant Human B7-H2-Fc Chimera
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Block - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by blocking the binding of 0.04 μg/mL Biotinylated Recombinant Human B7-H2 (ICOSL)-Fc chimera to 1 μg/mL immobilized human ICOS-Fc chimera. ND50 range: 0.01-0.07 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone W21036I completely blocks clone 9F.8A4 but does not block clone 2D3.
- RRID
-
AB_3083390 (BioLegend Cat. No. 387505)
AB_3083390 (BioLegend Cat. No. 387506)
Antigen Details
- Structure
- Homodimer
- Distribution
-
B cells, macrophages, monocytes, and dendritic cells
- Function
- The CD275/ICOS signal induces T cell proliferation and secretion of both Th1 and Th2 cytokines, such as IL-4 and IFN-γ, but not IL-2 (it also induces IL-5 and TNF-α production). CD275/ICOS signal may favor the induction of Th2-type responses and thus may be involved in the negative regulation of cell-mediated immune responses against tumors. CD275 is induced by TNF-α in B cells and monocytes and downregulated in dendritic cells.
- Interaction
- Activated T cells
- Ligand/Receptor
- ICOS
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
- Ishikawa K, et al. 1998. DNA Res. 5:169-76.
- Yoshinaga SK, et al. 2000. Int. Immunol. 12:1439-47.
- Flesch IE. 2003. J. Biol. Regul. Homeost. Agents. 16:217-9.
- Akbari O, et al. 2002. Nat. Med. 8:1024-32.
- Yao S, et al. 2011. Immunity. 34:729-40.
- Gene ID
- 23308 View all products for this Gene ID
- UniProt
- View information about CD275 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD275 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD275 (B7-H2, ICOSL) | W21036I | FC |
| PE anti-human CD275 (B7-H2, ICOSL) | W21036I | FC |
| Ultra-LEAF™ Purified anti-human CD275 (B7-H2, ICOSL) | W21036I | Block |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD275 (B7-H2, ICOSL)
Human peripheral blood lymphocytes were stained with purifie... -
PE anti-human CD275 (B7-H2, ICOSL)
Human peripheral blood lymphocytes were surface stained with... -
Ultra-LEAF™ Purified anti-human CD275 (B7-H2, ICOSL)
Biotinylated Recombinant Human B7-H2 (ICOSL)-Fc chimera (Cat...
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