- Clone
- MQ1-17H12 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interleukin-2, T cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF)
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells, promoting proliferation and maturation. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Cat, Chimpanzee, Baboon, Cynomolgus, Rhesus, Sooty Mangabey
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- E. coli - expressed recombinant human IL-2
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ELISA Capture - Quality tested
CyTOF® - Verified - Recommended Usage
-
This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
-
ELISA or ELISPOT Capture2,3: The purified MQ1-17H12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the Biotin anti-human IL-2 antibody (Cat. No. 517605) as the detecting antibody. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture. For ELISPOT capture applications, a concentration range of 4.0 - 8.0 µg/mL is recommended.
Additional reported applications (for the relevant formats) include: immunoprecipitation2, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections1,4-6,8, neutralization13, and immunocytochemistry.
Note: For testing human IL-2 in serum or plasma, BioLegend's LEGEND MAX™ Kit (Cat. No. 431807) is specially developed and recommended.
Clone MQ1-17H12 cross-reacts to Cat15 - Additional Product Notes
-
Maxpar® is a registered trademark of Standard BioTools Inc.
-
Application References
(PubMed link indicates BioLegend citation) -
- Andersson J, et al. 1994. Immunology 83:16. (IHC)
- Abrams J, et al. 1992. Immunol. Rev. 127:5. (IP)
- Abrams JS. 1995. Curr. Prot. Immunol. Unit 6.20.
- Fernandez V, et al. 1994. Eur. J. Immunol. 24:1808. (IHC)
- Skansen-Saphir U, et al. 1994. Eur. J. Immunol. 24:916. (IHC)
- Andersson U, et al. Detection and Quantification of Gene Expression. New York:Springer-Verlag. (IHC)
- Prussin C, et al. 1995. J. Immunol. Methods. 188:117.
- Raqib R, et al. 2002. Infect. Immun. 70:3199. (IHC)
- Dzhagalov I, et al. 2007. J. Immunol. 178:2113. PubMed
- Colleton BA, et al. 2009. J Virol. 83:6288. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Rout N, et al. 2010. PLoS One 5:e9787. (FC)
- Yeap SK, et al. 2013. BMC Complement Altern. Med. 13:145. (Neut)
- Wu Z, et al. 2015. J Virol. 89:6435. PubMed
- Maksaereekul S, et al. 2009. Vaccine. 28:3754 (FC) PubMed
- RRID
-
AB_2562839 (BioLegend Cat. No. 500339)
Antigen Details
- Structure
- Cytokine; 15.4 kD (Mammalian)
- Bioactivity
- Proliferation of T lymphocytes, B cells, anti-inflammatory, hematopoiesis, tumor surveillance
- Cell Sources
- T cells
- Cell Targets
- T cells, B cells, NK cells, LAK cells, monocytes, macrophages, oligodendrocytes
- Receptors
- High affinity heterotrimer of IL-2Rα/β/γ, intermediate affinity homodimer IL-2Rα (CD25; p55; Tac) and heterodimer IL-2Rβ (CD122)/γ; γ-subunit (CD132) in common with IL-4R, IL-7R, IL-13R, IL-15R
- Cell Type
- Tregs
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Taniguchi T, et al. 1993. Cell 73:5.
3. Nistico G. 1993. Prog. Neurobiol. 40:463.
4. Waldmann T, et al. 1993. Ann. NY Acad. Sci. 685:603. - Regulation
- Upregulated by NFAT; downregulated by TCF-8 and CIF (colostrums inhibitory factor)
- Gene ID
- 3558 View all products for this Gene ID
- UniProt
- View information about IL-2 on UniProt.org
Related FAQs
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
-
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
-
We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
-
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
-
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Other Formats
View All IL-2 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
APC anti-human IL-2
PMA + ionomycin-stimulated (6 hours) human peripheral blood ... -
FITC anti-human IL-2
5 hours PMA + ionomycin-stimulated human peripheral blood ly... -
PE anti-human IL-2
PMA/Ionomycin-stimulated human PBMCs were stained with CD3 P... -
Purified anti-human IL-2
-
Alexa Fluor® 488 anti-human IL-2
PMA+ionomycin-stimulated (6 hours) human peripheral blood ly... -
Alexa Fluor® 647 anti-human IL-2
PMA+ionomycin-stimulated (5 hours) human PBMCs surface stain... -
Alexa Fluor® 700 anti-human IL-2
PMA+ionomycin-stimulated (5 hours) human PBMCs surface stain... -
PerCP/Cyanine5.5 anti-human IL-2
PMA + ionomycin-stimulated (6 hours) human peripheral blood ... -
Pacific Blue™ anti-human IL-2
PMA+ionomycin-stimulated (5 hours) human PBMCs surface stain... PMA+ionomycin-stimulated (5 hours) human PBMCs surface stain... -
PE/Cyanine7 anti-human IL-2
PMA+ionomycin-stimulated (6 hours) peripheral blood lymphocy... -
Brilliant Violet 421™ anti-human IL-2
Human peripheral blood lymphocytes were stimulated with PMA ... -
Brilliant Violet 605™ anti-human IL-2
Human peripheral blood lymphocytes were stimulated with PMA ... -
Brilliant Violet 650™ anti-human IL-2
PMA+ionomycin-stimulated human peripheral blood lymphocytes ... -
Brilliant Violet 510™ anti-human IL-2
PMA + Ionomycin-stimulated (6 hours) human peripheral blood ... -
Brilliant Violet 711™ anti-human IL-2
PMA + ionomycin-stimulated (6 hours) human peripheral blood ... -
APC/Cyanine7 anti-human IL-2
Human peripheral blood lymphocytes were stimulated with PMA ... -
Purified anti-human IL-2 (Maxpar® Ready)
Human PBMCs were incubated for 5 hours in media alone (top) ... -
PE/Dazzle™ 594 anti-human IL-2
PMA + ionomycin-stimulated (6 hours) human peripheral blood ... -
Brilliant Violet 785™ anti-human IL-2
Human peripheral blood lymphocytes were stimulated with PMA ... -
PerCP anti-human IL-2
PMA + ionomycin stimulated (6 hours) human peripheral blood ... -
APC/Fire™ 750 anti-human IL-2
PMA + ionomycin stimulated (6 hours) human peripheral blood ... -
Ultra-LEAF™ Purified anti-human IL-2
-
APC/Fire™ 810 anti-human IL-2
PMA + ionomycin stimulated (6 hours) human peripheral blood ... -
TotalSeq™-B1005 anti-human IL-2
-
Brilliant Violet 750™ anti-human IL-2
PMA+ ionomycin stimulated (6 hours) human peripheral blood l... -
Spark UV™ 387 anti-human IL-2
Human peripheral blood lymphocytes were stimulated with Cell...
Follow Us