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Purified anti-ILK Antibody

Pricing & Availability
Clone
P83A9 (See other available formats)
Regulatory Status
RUO
Other Names
Integrin-linked protein kinase, 59 kDa serine/threonine-protein kinase, ILK-1, p59ILK
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_P83A9_PURE_ILK_Antibody_1_WB_110117
Total lysates (15µg protein) from HeLa and HeLa ILK CRISPR/Cas9 knockout (KO) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1µg/ml) purified anti-ILK antibody , clone P83A9 (upper). Proteins were visualized using chemiluminescence detection by incubation with HRP goat anti-mouse-IgG secondary antibody (cat#405306). Direct-Blot™ HRP anti-β-actin Antibody (Cat#643807) was used as a loading control (lower). Lane M is the Molecular weight ladder.
  • 1_P83A9_PURE_ILK_Antibody_1_WB_110117
    Total lysates (15µg protein) from HeLa and HeLa ILK CRISPR/Cas9 knockout (KO) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1µg/ml) purified anti-ILK antibody , clone P83A9 (upper). Proteins were visualized using chemiluminescence detection by incubation with HRP goat anti-mouse-IgG secondary antibody (cat#405306). Direct-Blot™ HRP anti-β-actin Antibody (Cat#643807) was used as a loading control (lower). Lane M is the Molecular weight ladder.
  • 2_P83A9_PURE_ILK_Antibody_1_WB_012017
    Zebra fish tissue lysate and total cell lysates (15µg protein) from SH-SY5Y, HeLa and NIH3T3 were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with 0.5 µg/mL purified anti-ILK (clone P83A9) antibody (upper). Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (middle) and Ponceau S staining (lower) were used as a loading controls.
  • 3_P83A9_PURE_ILK_Antibody_2_WB_012017
    HeLa cells were fixed with 4% PFA for fifteen minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 2 µg/mL of purified anti-ILK antibody (clone P83A9) overnight at 4°C followed by Alexa Fluor® 594 (red) conjugated goat anti-mouse IgG antibody for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
See high resolution WB/IF data...
Cat # Size Price Save
694901 25 µg ¥22,220
694902 100 µg ¥60,720
Description

Integrin-linked kinase (ILK) is an integrin associated actin and tubulin cytoskeletal interacting effector. ILK was identified in a search for proteins interacting with integrin β CTs. It has an N-terminal ankyrin repeat domain (ARD) and a C-terminal integrin β CT binding kinase-like domain (KLD). ILK functions as a key scaffold at cell-ECM adhesions and regulates integrin-mediated as well as growth factor regulated functions. ILK orchestrates several signaling pathways, such as PI3Kinase/Akt, Wnt, TGF-β, Hippo tumor suppressor pathway and epithelial-mesenchymal transition signaling in various types of cancer cells. ILK expression is upregulated in many types of cancers.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant of ILK (aa: 97-224) expressed in E.Coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2650742 (BioLegend Cat. No. 694901)
AB_2650742 (BioLegend Cat. No. 694902)

Antigen Details

Structure
452 amino acids, and predicted molecular weight is 51.4 kD.
Distribution

Cytoplasm

Function
Receptor-proximal protein kinase regulating integrin-mediated signal transduction.
Interaction
Interacts with ITGB1, ITGB2, ITGB3 and/or ITGB5, LIMS1 and LIMS2, PARVA and PARVB, EPHA1.
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Hannigan GE, et al. 1996. Nature 379:91.
2. Serrano I, et al. 2013. Oncogene 32: 50.
3. Hannigan G, et al. 2005. Nat. Rev. Cancer 5: 51.
4. McDonald PC, et al. 2008. J. Cell Sci. 121: 3121.
5. Serrano I, et al. 2013. Nat. Commun. 4:2976.
6. Gil D, et al. 2016. Tumor Biol. 37:15185.

Gene ID
3611 View all products for this Gene ID
UniProt
View information about ILK on UniProt.org

Other Formats

View All ILK Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-ILK P83A9 WB,KO/KD-WB,ICC
Go To Top Version: 2    Revision Date: 11/01/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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