- Clone
- AFS98 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CSF-1R, M-CSFR, c-fms
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
CSF-1R, also known as CD115 and M-CSFR, is a single-pass type I membrane protein and member of the platelet-derived growth factor receptor family. This c-fms (Fms proto-oncogene) gene product's natural ligands include M-CSF and IL-34. Structural studies of CD115 have described an Ig-like extracellular domain, a transmembrane domain, an intracellular juxtamembrane domain, a split tyrosine kinase domain, and a C-terminal tail receptor. Receptor activation induces homodimerization in addition to phosphorylation and ubiquitination of intracellular residues. CD115 directly influences tissue macrophage and osteoclast differentiation and proliferation. It is expressed on monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, and osteoclasts.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
CyTOF® - Verified - Recommended Usage
-
This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
-
Additional reported applications (for the relevant formats) include: blocking of ligand binding1. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays.
It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature. Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation. Pre-treatment with EDTA and low temperatures (2 to 8°C) helps in maintaining surface expression of CD1154. - Additional Product Notes
-
Maxpar® is a registered trademark of Standard BioTools Inc.
-
Application References
(PubMed link indicates BioLegend citation) - Product Citations
-
- RRID
-
AB_2563709 (BioLegend Cat. No. 135521)
Antigen Details
- Structure
- Ig superfamily, 145 kD
- Distribution
-
Monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, osteoclasts
- Function
- Growth factor receptor, tyrosine kinase
- Ligand/Receptor
- Macrophage colony stimulating factor (M-CSF), IL-34
- Cell Type
- Dendritic cells, Macrophages, Monocytes, Osteoclasts
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
- Sudo T, et al. 1995 Oncogene 11:2469.
- Murayama T, et al. 1999 Circulation 99:1740.
- Goswami S, et al. 2005 Cancer Res. 65:5278.
- Yu W, et al. 2008 J. Leuko. Biol. 84:852.
- Gene ID
- 12978 View all products for this Gene ID
- UniProt
- View information about CD115 on UniProt.org
Related FAQs
- Why do I have a weak CD115 staining?
-
It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature. Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation. Pre-treatment with EDTA and low temperatures (2 - 8°C) helps in maintaining surface expression of CD115. In addition, brief fixation of the cells with Fixation Buffer (Cat. No. 420801) for 10 minutes will block CD115 internalization.
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
-
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
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We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
-
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
-
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Other Formats
View All CD115 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD115 (CSF-1R)
Thioglycolate-elicited BALB/c mouse peritoneal macrophages s... Thioglycolate-elicited BALB/c mouse peritoneal macrophages s... -
PE anti-mouse CD115 (CSF-1R)
Thioglycolate-elicited BALB/c mouse peritoneal macrophages s... Thioglycolate-elicited BALB/c mouse peritoneal macrophages s... -
Biotin anti-mouse CD115 (CSF-1R)
Thioglycolate-elicited BALB/c mouse peritoneal macrophages s... -
APC anti-mouse CD115 (CSF-1R)
Thioglycolate-elicted BALB/c mouse peritoneal macrophages st... -
Alexa Fluor® 488 anti-mouse CD115 (CSF-1R)
Thioglycolate-elicited C57BL/6 mouse peritoneal macrophages ... -
Brilliant Violet 421™ anti-mouse CD115 (CSF-1R)
Thioglycolate-elicited C57BL/6 mouse peritoneal macrophages ... -
Brilliant Violet 605™ anti-mouse CD115 (CSF-1R)
Thioglycolate-elicited C57BL/6 mouse peritoneal macrophages ... -
Brilliant Violet 711™ anti-mouse CD115 (CSF-1R)
Thioglycolate-elicited C57BL/6 mouse peritoneal macrophages ... -
Alexa Fluor® 594 anti-mouse CD115 (CSF-1R)
C57BL/6 mouse bone marrow derived macrophages were fixed wit... -
Purified anti-mouse CD115 (CSF-1R) (Maxpar® Ready)
Mouse J774A.1 macrophage cells (top) and mouse EL4 T cells (... -
PE/Cyanine7 anti-mouse CD115 (CSF-1R)
Thioglycolate-elicited BALB/c peritoneal macrophages were st... -
PerCP/Cyanine5.5 anti-mouse CD115 (CSF-1R)
Thioglycolate-elicited BALB/c peritoneal macrophages were st... -
PE/Dazzle™ 594 anti-mouse CD115 (CSF-1R)
Thioglycolate-elicited BALB/c mouse peritoneal macrophages w... -
Alexa Fluor® 647 anti-mouse CD115 (CSF-1R)
Thioglycolate-elicited BALB/c mouse peritoneal macrophages w... -
APC/Cyanine7 anti-mouse CD115 (CSF-1R)
Thioglycolate-elicited BALB/c mouse peritoneal macrophages w... -
TotalSeq™-A0105 anti-mouse CD115 (CSF-1R)
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APC/Fire™ 750 anti-mouse CD115 (CSF-1R)
Thioglycolate-elicited C57BL/6 mouse peritoneal macrophages ... -
Ultra-LEAF™ Purified anti-mouse CD115 (CSF-1R)
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TotalSeq™-B0105 anti-mouse CD115 (CSF-1R)
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TotalSeq™-C0105 anti-mouse CD115 (CSF-1R)
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Brilliant Violet 785™ anti-mouse CD115 (CSF-1R)
Thioglycolate-elicited BALB/c peritoneal macrophages were st...
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