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- Clone
- C71/16 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Integrin beta-2, Mac-1 beta, macrophage antigen-1 beta, complement receptor C3 subunit beta, lymphocyte function associated antigen 1, cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta, 2E6, LAD, Cd18, Lfa1, MF17, LCAMB, AI528527
- Previously
-
Covance Catalog# MRT-5214
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
C57BL/6 mouse splenocytes were stained with purified anti-mouse CD18 (clone C71/16) (filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by goat anti-rat IgG APC.
CD18 is a 95 kD protein, also known as integrin β2 subunit. It is expressed on all leukocytes. CD18, in association with integrin α chain CD11a, CD11b, and CD11c forms LFA-1, Mac-1, and αXβ2, respectively, and plays an important role in leukocytes adhesion. The CD18 integrin complexes bind ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, and fibrinogen. The M18/2 antibody is able to block tumor cell metastasis and enhance cell adhesion.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- This monoclonal antibody was raised against cell membrane glycoproteins derived from T-cell lymphoma BW5147 cells.
- Formulation
- Phosphate-buffered solution.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/ml
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
-
FC
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
The optimal working dilution should be determined for each specific assay condition.
• FC: 1:1000 - 1:10,000* - Application Notes
-
This antibody is effective in flow cytometry (FC).
*FC Positive Control: Mouse blood
Rat IgG2a Isotype antibody available: Catalog number: 910601 -
Application References
(PubMed link indicates BioLegend citation) -
- Trowbridge IS, et al. Molecular Complexity of Leukocyte Surface Glycoproteins Related to the Macrophage Differentiation Antigen MAC-1. J. Exp. Med. 154: 1517-1524, November 1981.
- RRID
-
AB_2564939 (BioLegend Cat. No. 830501)
Antigen Details
- Biology Area
- Cell Biology, Complement, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Anderson DC, et al. 1987. Annu. Rev. Med. 38:175.
3. Wilson RW, et al. 1993. J. Immunol. 151:1571. - Gene ID
- 16414 View all products for this Gene ID
- UniProt
- View information about CD18 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
Other Formats
View All CD18 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse CD18 | C71/16 | FC |
| Brilliant Violet 421™ anti-mouse CD18 | C71/16 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD18
C57BL/6 mouse splenocytes were stained with purified anti-mo... -
Brilliant Violet 421™ anti-mouse CD18
C57BL/6 mouse splenocytes were stained with anti-mouse CD18...
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