Purified anti-mouse CD209a DC-SIGN Antibody anti-CD209a

Clone: MMD3 (See other available formats)
Regulatory Status: RUO
Other Names: CD209 antigen-like protein A, dendritic cell-specific ICAM-3-grabbing non-integrin, CIRE, CD209, CDSIGN
Previously: Covance Catalog# MMS-5224
Isotype: Mouse IgG2c, κ
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Product Citations: publications

MMD3_Purified_CD209a_Antibody_FC_081017_updated — CHO-DC-SIGN transfected (filled histogram) & CHO-K1 (dashed histogram) cell lines were stained with Purified anti-mouse CD209a (clone MMD3) antibody, followed by PE anti-mouse IgG2a antibody (clone RMG2a-62).

Cat #	Size	Price	Save
833001	100 µL	¥60,500

Description

CD209a, is also known as dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) and CIRE. Of the eight DC-SIGN-related receptors in mice, CD209a is considered the closest homolog to human DC-SIGN (CD209). Although both mouse and human counterparts are expressed on monocyte-derived dendritic cells, expression variation has been noted. Mouse CD209a is found on skin and splenic myeloid cells and is highly expressed on plasmacytoid dendritic cells, while human CD209 is expressed on myeloid dendritic cells, placental macrophages, liver and placental endothelial cells. CD209 binds to ICAM-3 (CD50), ICAM-2 (CD102), and Butyrophilin (BTN2A1), and mediates dendritic cell migration and T cell proliferation. It plays a critical role in capturing and internalizing pathogens such as Streptococcus pneumoniae.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This monoclonal antibody was raised against a purified extracellular domain of mouse CD209a (DC-SIGN)-IgG1 Fc fusion protein.
Formulation: Phosphate-buffered solution.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/ml
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: FC
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.

The optimal working dilution should be determined for each specific assay condition.
• FC: 1 μg/test
Application Notes: This antibody is effective in Flow Cytometry (FC).

FC Positive Control: Mouse splenocytes
RRID: AB_2564962 (BioLegend Cat. No. 833001)

Antigen Details

Biology Area: Cell Biology, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules
Gene ID: 170786 View all products for this Gene ID
UniProt: View information about CD209a on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD209a (DC-SIGN) Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse CD209a (DC-SIGN)	MMD3	FC
PE anti-mouse CD209a (DC-SIGN)	MMD3	FC
APC anti-mouse CD209a (DC-SIGN)	MMD3	FC
Brilliant Violet 421™ anti-mouse CD209a (DC-SIGN)	MMD3	FC
TotalSeq™-B1282 anti-mouse CD209a (DC-SIGN)	MMD3	PG

Certificate of Analysis

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Go To Top Version: 5 Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

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