- Clone
- 1A8 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Lymphocyte antigen 6 complex, locus G
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Ly-6G transfected EL-4J cell line.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
CyTOF®, WB - Verified - Recommended Usage
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This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
-
While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.
Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, depletion4, 12-14, and spatial biology (IBEX)20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662). - Additional Product Notes
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Maxpar® is a registered trademark of Standard BioTools Inc.
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Application References
(PubMed link indicates BioLegend citation) -
- Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
- Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
- Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
- Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
- Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
- Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
- Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
- Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
- Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
- Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
- Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
- Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
- Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
- Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
- Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
- Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
- Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
- McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
- Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
- Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
-
- RRID
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AB_2563784 (BioLegend Cat. No. 127637)
Antigen Details
- Structure
- A 21-35 kD GPI-anchorded membrane protein
- Distribution
-
Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.
- Cell Type
- Granulocytes, Macrophages, Monocytes
- Biology Area
- Immunology, Innate Immunity
- Antigen References
-
Fleming TJ, et al. 1993. J. Immunol. 151:2399.
- Gene ID
- 546644 View all products for this Gene ID
- UniProt
- View information about Ly-6G on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
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We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
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We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
-
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
-
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Alexa Fluor® 594 anti-mouse Ly-6G
C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
Purified anti-mouse Ly-6G
C57BL/6 bone marrow cells stained with 1A8 purified, followe... Fresh, frozen mouse spleen was stained with purified Ly6G cl... Mouse uterine tissue fixed in 10% formalin, paraffin embedde... -
Biotin anti-mouse Ly-6G
C57BL/6 bone marrow cells stained with 1A8 biotin, followed ... -
FITC anti-mouse Ly-6G
BALB/cmouse bone marrow cells stained with 1A8 FITC (gated o... -
PE anti-mouse Ly-6G
C57BL/6 bone marrow cells stained with 1A8 PE (gated on myel... -
Alexa Fluor® 647 anti-mouse Ly-6G
C57BL/6 mouse bone marrow cells stained with 1A8 Alexa Fluor... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
Pacific Blue™ anti-mouse Ly-6G
C57BL/6 bone marrow cells stained with 1A8 Pacific Blue&trad... -
APC anti-mouse Ly-6G
C57BL/6 bone marrow cells stained with 1A8 APC -
PerCP/Cyanine5.5 anti-mouse Ly-6G
C57BL/6 bone marrow cells stained with 1A8 PerCP/Cyanine5.5 -
PE/Cyanine7 anti-mouse Ly-6G
C57BL/6 bone marrow cells stained with 1A8 PE/Cyanine7 -
Alexa Fluor® 700 anti-mouse Ly-6G
C57BL/6 bone marrow cells stained with 1A8 Alexa Fluor® ... -
APC/Cyanine7 anti-mouse Ly-6G
C57BL/6 bone marrow cells stained with 1A8 APC/Cyanine7 -
Alexa Fluor® 488 anti-mouse Ly-6G
C57BL/6 mouse bone marrow cells were stained with Ly-6G (clo... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... Confocal image of C57BL/6 mouse lung sample acquired using t... -
Brilliant Violet 421™ anti-mouse Ly-6G
C57BL/6 mouse bone marrow cells were stained with Ly-6G (clo... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
Brilliant Violet 570™ anti-mouse Ly-6G
C57BL/6 bone marrow cells were stained with Ly-6G (clone 1A8... -
Ultra-LEAF™ Purified anti-mouse Ly-6G
C57BL/6 bone marrow cells stained with 1A8 purified, followe... Mouse uterine tissue fixed in 10% formalin, paraffin embedde... -
Brilliant Violet 510™ anti-mouse Ly-6G
C57BL/6 mouse bone marrow cells were stained with Ly-6G (clo... -
Purified anti-mouse Ly-6G (Maxpar® Ready)
C57BL/6 mouse bone marrow cells stained with 148Nd-anti-CD11... Western blot analysis of mouse bone marrow (lane 1), mouse s... -
Brilliant Violet 650™ anti-mouse Ly-6G
C57BL/6 mouse bone marrow cells were stained with Ly-6G (clo... -
Brilliant Violet 711™ anti-mouse Ly-6G
C57BL/6 mouse bone marrow cells were stained with Ly-6G (clo... -
Brilliant Violet 605™ anti-mouse Ly-6G
C57BL/6 mouse bone marrow cells were stained with Ly-6G (clo... -
Brilliant Violet 785™ anti-mouse Ly-6G
C57BL/6 mouse bone marrow cells were stained with Ly-6G (clo... -
PE/Dazzle™ 594 anti-mouse Ly-6G
C57BL/6 mouse bone marrow cells were stained with Ly-6G (clo... -
APC/Fire™ 750 anti-mouse Ly-6G
C57BL/6 mouse bone marrow cells were stained with Ly-6G (clo... -
PerCP anti-mouse Ly-6G
C57BL/6 mouse bone marrow cells were stained with Ly-6G (clo... -
TotalSeq™-A0015 anti-mouse Ly-6G
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TotalSeq™-C0015 anti-mouse Ly-6G
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TotalSeq™-B0015 anti-mouse Ly-6G
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Spark Blue™ 550 anti-mouse Ly-6G
C57BL/6 mouse bone marrow cells stained with Ly-6G (clone 1A... -
Spark NIR™ 685 anti-mouse Ly-6G
C57BL/6 mouse bone marrow cells were stained with Ly-6G (clo... -
Spark YG™ 593 anti-mouse Ly-6G
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
APC/Fire™ 810 anti-mouse Ly-6G Antibody
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
PE/Cyanine5 anti-mouse Ly-6G
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
PE/Fire™ 810 anti-mouse Ly-6G Antibody
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
Spark UV™ 387 anti-mouse Ly-6G
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
PE/Fire™ 640 anti-mouse Ly-6G
C57BL/6 mouse bone marrow cells stained with anti-mouse Ly-6... -
Spark YG™ 570 anti-mouse Ly-6G
C57BL/6 mouse bone marrow cells were stained with anti-mouse... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... C57BL/6 mouse frozen cerebellum section was fixed with 4% pa... -
Spark Red™ 718 anti-mouse Ly-6G (Flexi-Fluor™)
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Spark Blue™ 574 anti-mouse Ly-6G (Flexi-Fluor™)
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