Loading...

PerCP/Cyanine5.5 anti-mouse Ly-6G Antibody

Pricing & Availability
Clone
1A8 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus G
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
1A8_PerCPCy55_091809
C57BL/6 bone marrow cells stained with 1A8 PerCP/Cyanine5.5
  • 1A8_PerCPCy55_091809
    C57BL/6 bone marrow cells stained with 1A8 PerCP/Cyanine5.5
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Save
127615 25 µg ¥19,940
127616 100 µg ¥61,770
Description

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly-6G transfected EL-4J cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.

Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, depletion4, 12-14, and spatial biology (IBEX)20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).

Application References

(PubMed link indicates BioLegend citation)
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
  2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
  3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
  4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
  5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
  6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
  7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
  8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
  10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
  11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
  12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
  13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
  14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
  15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
  17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
  19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
  20. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  21. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Bruno MEC, et al. 2022. J Cell Physiol. 237:706. PubMed
  2. Ozga AJ, et al. 2022. Immunity. 55:82. PubMed
  3. Merana GR, et al. 2022. Cell Rep. 39:110891. PubMed
  4. Dolfi B, et al. 2022. Cell Rep. 39:110949. PubMed
  5. Ostendorf BN, et al. 2022. Nature. 611:346. PubMed
  6. Gonçalves R, et al. 2023. iScience. 26:105972. PubMed
  7. Marritt KL, et al. 2023. Front Immunol. 13:1087991. PubMed
  8. Denis M, et al. 2023. Front Immunol. 13:1011943. PubMed
  9. Ryan CB, et al. 2022. Neurobiol Dis. 163:105608. PubMed
  10. Fernández-García V, et al. 2022. Biomed Pharmacother. 148:112769. PubMed
  11. Wong Fok Lung T, et al. 2022. Cell Metab. 34:761. PubMed
  12. Zhang L, et al. 2022. Immunol Cell Biol. 100:691. PubMed
  13. Kedmi R, et al. 2022. Nature. 610:737. PubMed
  14. Chang CY, et al. 2022. Sci Adv. 8:eabq0615. PubMed
  15. Gao L, et al. 2023. J Nanobiotechnology. 21:56. PubMed
  16. Vanneste D, et al. 2023. Nat Immunol. 24:827. PubMed
  17. Dijkman K, et al. 2023. NPJ Vaccines. 8:66. PubMed
  18. Delvecchio FR, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:1543. PubMed
  19. Ercoli G, et al. 2021. Front Immunol. 11:611661. PubMed
  20. Wong Fok Lung T, et al. 2020. Nat Microbiol. 141:5. PubMed
  21. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  22. Cho SM et al. 2018. Journal of neurochemistry. 148(5):625-638 . PubMed
  23. Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed
  24. Pan Y, et al. 2021. Front Cell Neurosci. 15:664312. PubMed
  25. Su N, Xiong Y 2016. Sci Rep. 6:22658. PubMed
  26. Kwon O, et al. 2016. Stem Cell Res. 16: 682-691. PubMed
  27. Kim AS, et al. 2021. Cell. 184(17):4414-4429.e19. PubMed
  28. Maschmeyer P, et al. 2017. J Autoimmun.. 10.1016/j.jaut.2017.11.005. PubMed
  29. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
  30. Merz SF, et al. 2019. Nat Commun. 10:2312. PubMed
  31. Plumlee CR, et al. 2020. Cell Host Microbe. 29(1):68-82.e5. PubMed
  32. Liang W, et al. 2020. Nat Commun. 4.563888889. PubMed
  33. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
  34. Leech JM, et al. 2020. Cell Host & Microbe. 26(6):795-809.e5.. PubMed
  35. Haertel E, et al. 2018. Eur J Immunol. 48:1001. PubMed
  36. Park JA, et al. 2021. J Hematol Oncol. 14:142. PubMed
  37. Mukherjee D, et al. 2022. Nat Commun. 13:3747. PubMed
  38. Kwon O, et al. 2014. Proc Natl Acad Sci U S A. 111:592. PubMed
  39. Liu Y, et al. 2015. Sci Rep. 5:17215. PubMed
  40. Fuster JJ, et al. 2020. Cell Rep. 33:108326. PubMed
  41. Uroda T, et al. 2020. Nat Protoc. 15:2107. PubMed
  42. Francian A, et al. 2021. J Drug Target. 29:754. PubMed
  43. Wang C, et al. 2021. Cell Rep. 37:110021. PubMed
  44. McCullough LD, et al. 2021. J Clin Invest. 131:. PubMed
  45. Sugita J, et al. 2021. Nat Commun. 12:1910. PubMed
  46. Klopfenstein N, et al. 2021. PLoS Pathog. 17:e1009387. PubMed
  47. Ostendorf BN, et al. 2020. Nat Med. 26:1048. PubMed
  48. Lebratti T, et al. 2021. eLife. 10:00. PubMed
  49. Bhattacherjee A, et al. 2019. Commun Biol. 2:450. PubMed
  50. Privratsky JR, et al. 2018. Am J Physiol Renal Physiol. 315:F682. PubMed
  51. Schlegel M, et al. 2018. J Clin Invest. 128:4711. PubMed
  52. Dane EL, et al. 2022. Nat Mater. 21:710. PubMed
  53. Moses K, et al. 2016. J Leukoc Biol. 99: 811 - 823. PubMed
  54. Zhang B, et al. 2016. Mol Cell. 63: 976-89. PubMed
  55. Doty DT, et al. 2020. Int J Mol Sci. 21:00. PubMed
  56. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  57. Tomlinson KL, et al. 2021. Nat Commun. 12:1399. PubMed
  58. Kovacic B, et al. 2014. Haematologica. 99:1006. PubMed
  59. Funk KE, et al. 2019. J Neuroinflammation. 16:22. PubMed
  60. Hildebrand KM, et al. 2021. PLoS One. 16:e0253864. PubMed
  61. Zhang T, et al. 2022. Oncogene. . PubMed
  62. Riquelme SA, et al. 2020. Cell Metabolism. 31(6):1091-1106.e6. PubMed
  63. Körner A, et al. 2019. Nat Commun. 10:633. PubMed
  64. Zhang Y, et al. 2021. J Immunol Res. 2021:5599439. PubMed
  65. Reinfeld BI, et al. 2021. Nature. 593:282. PubMed
  66. Dang MT, et al. 2021. Cell Reports. 34(13):108917. PubMed
  67. Körner A, et al. 2019. Proc Natl Acad Sci U S A. 116:20623. PubMed
  68. Acker KP, et al. 2019. iScience. 19:281. PubMed
  69. Niknam S, et al. 2018. Clin Cancer Res. 24:5735. PubMed
  70. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  71. Devaraj A, et al. 2021. J Clin Invest. 131:. PubMed
  72. Gurung P, et al. 2016. Proc Natl Acad Sci U S A. 113: 4452-4457. PubMed
  73. Puigdelloses M, et al. 2021. J Immunother Cancer. 9:. PubMed
  74. Maisel D, et al. 2016. PLoS One. 11: 0159716. PubMed
  75. Ballet R, et al. 2014. PLoS Pathog. 10:1004550. PubMed
  76. Dang MT, et al. 2021. STAR Protoc. 2:100957. PubMed
  77. Li D, et al. 2022. Emerg Microbes Infect. 11:2248. PubMed
  78. Garcia-Bonilla L, et al. 2014. J Immunol. 193:2531. PubMed
  79. Shepardson K, et al. 2016. MBio. 7: e00506-16. PubMed
  80. Alam Z, et al. 2020. Cell Rep. 107825:31. PubMed
  81. Mattila JT, et al. 2017. J Immunol. 199:806. PubMed
  82. Blagih J, et al. 2020. Cell Rep. 30:481. PubMed
  83. Klein JC, et al. 2017. Nat Commun. 8:14600. PubMed
  84. Desai P, et al. 2021. Cell. 184(5):1214-1231.e16. PubMed
  85. Xu G, et al. 2020. Cell Rep. 32:108170. PubMed
  86. Chen Z, et al. 2016. Nat Commun. 7:11596. PubMed
  87. Lokken K, et al. 2014. PLoS Pathog. 10:1004049. PubMed
  88. Muri J, et al. 2020. eLife. 9:e53627.. PubMed
  89. Sun X, et al. 2020. Cell Metabolism. 31(1):189-206.e8.. PubMed
  90. Yeung F, et al. 2020. Cell Host & Microbe. 27(5):809-822. PubMed
  91. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  92. Chan WY, et al. 2019. Infect Immun. 87:. PubMed
  93. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  94. Karki R, et al. 2020. bioRxiv. . PubMed
  95. Bennett FC, et al. 2018. Neuron. 98:1170. PubMed
  96. Dymek B, et al. 2022. J Inflamm Res. 15:5621. PubMed
  97. Milich LM, et al. 2021. J Exp Med. 218:. PubMed
  98. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  99. Karki R, et al. 2021. Cell. 184:149. PubMed
  100. Jablonski K, et al. 2015. PLoS One. 10: 0145342. PubMed
  101. Salazar V, et al. 2019. Cell Rep. 26:1585. PubMed
  102. Deliyannis G, et al. 2021. JCI Insight. 6:. PubMed
  103. Fedele C, et al. 2021. J Exp Med. 218: . PubMed
  104. Dai X, et al. 2021. Molecular Cell. 81(11):2317-2331.e6. PubMed
  105. Roediger B et al. 2018. Cell. 175(2):530-543 . PubMed
  106. Ireland L, et al. 2020. Front Immunol. 11:297. PubMed
  107. Seenappa LM, et al. 2022. NPJ Vaccines. 7:128. PubMed
  108. Verma M, et al. 2021. J Exp Med. 218:. PubMed
  109. Nakayama Y, et al. 2020. Proc Natl Acad Sci U S A. 117:14365. PubMed
  110. Garcia-Bonilla L, et al. 2015. J Neurosci. 35: 14783 - 14793. PubMed
  111. Zhu Y et al. 2017. The Journal of Neuroscience. 37(9):2362-2376 . PubMed
  112. Hohsfield LA, et al. 2021. Elife. 10:. PubMed
  113. Yang F, et al. 2021. Nat Commun. 12:3424. PubMed
  114. Trivedi S, et al. 2020. Elife. 9:00. PubMed
  115. Amber Smith, Amanda Smith 2016. Sci Rep. 6:38703. PubMed
  116. Kim I et al. 2015. Brain and behavior. 5(12):e00403 . PubMed
  117. Syed I et al. 2018. Cell metabolism. 27(2):419-427 . PubMed
RRID
AB_1877271 (BioLegend Cat. No. 127615)
AB_1877271 (BioLegend Cat. No. 127616)

Antigen Details

Structure
A 21-35 kD GPI-anchorded membrane protein
Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type
Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID
546644 View all products for this Gene ID
UniProt
View information about Ly-6G on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All Ly-6G Reagents Request Custom Conjugation
Description Clone Applications
Alexa Fluor® 594 anti-mouse Ly-6G 1A8 IHC-F,SB
Purified anti-mouse Ly-6G 1A8 FC,IHC-F,IHC-P,SB
Biotin anti-mouse Ly-6G 1A8 FC,IHC
FITC anti-mouse Ly-6G 1A8 FC
PE anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 647 anti-mouse Ly-6G 1A8 FC,IHC-F,SB
Pacific Blue™ anti-mouse Ly-6G 1A8 FC
APC anti-mouse Ly-6G 1A8 FC
PerCP/Cyanine5.5 anti-mouse Ly-6G 1A8 FC
PE/Cyanine7 anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 700 anti-mouse Ly-6G 1A8 FC,IHC
APC/Cyanine7 anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 488 anti-mouse Ly-6G 1A8 FC,IHC-F,SB
Brilliant Violet 421™ anti-mouse Ly-6G 1A8 FC,IHC-F
Brilliant Violet 570™ anti-mouse Ly-6G 1A8 FC
Ultra-LEAF™ Purified anti-mouse Ly-6G 1A8 FC,Depletion,IHC
Brilliant Violet 510™ anti-mouse Ly-6G 1A8 FC
Purified anti-mouse Ly-6G (Maxpar® Ready) 1A8 FC,CyTOF®,WB
Brilliant Violet 650™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 711™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 605™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 785™ anti-mouse Ly-6G 1A8 FC
PE/Dazzle™ 594 anti-mouse Ly-6G 1A8 FC
APC/Fire™ 750 anti-mouse Ly-6G 1A8 FC
PerCP anti-mouse Ly-6G 1A8 FC
TotalSeq™-A0015 anti-mouse Ly-6G 1A8 PG
TotalSeq™-C0015 anti-mouse Ly-6G 1A8 PG
TotalSeq™-B0015 anti-mouse Ly-6G 1A8 PG
Spark Blue™ 550 anti-mouse Ly-6G 1A8 FC
Spark NIR™ 685 anti-mouse Ly-6G 1A8 FC
Spark YG™ 593 anti-mouse Ly-6G 1A8 FC
APC/Fire™ 810 anti-mouse Ly-6G Antibody 1A8 FC
PE/Cyanine5 anti-mouse Ly-6G 1A8 FC
PE/Fire™ 810 anti-mouse Ly-6G Antibody 1A8 FC
Spark UV™ 387 anti-mouse Ly-6G 1A8 FC
PE/Fire™ 640 anti-mouse Ly-6G 1A8 FC
Spark YG™ 570 anti-mouse Ly-6G 1A8 IHC-F,FC
Spark Red™ 718 anti-mouse Ly-6G (Flexi-Fluor™) 1A8 FC
Spark Blue™ 574 anti-mouse Ly-6G (Flexi-Fluor™) 1A8 FC
Go To Top Version: 3    Revision Date: 01/06/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account