Western blot of purified anti-Parkin antibody (clone A15165D). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Western blot of purified anti-Parkin antibody (clone A15165D). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
IHC staining of purified anti-Parkin antibody (clone A15165D) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
IHC staining of purified anti-Parkin antibody (clone A15165D) on formalin-fixed paraffin-embedded Parkinson’s disease substantia nigra tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
IHC staining of purified anti-Parkin antibody (clone A15165D) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, Cat. No. 929701) and Boost DAB Enhancer (Cat. No. 926701) were used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
IHC staining of purified anti-Parkin antibody (clone A15165D) on formalin-fixed paraffin-embedded Parkinson’s disease substantia nigra tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, Cat. No. 929701) and Boost DAB Enhancer (Cat. No. 926701) were used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Parkin (also known as PARK2, AR-JP, PRKN) is a protein encoded by the PARK2 gene in humans. It is a component of the E3 ubiquitin ligase complex that mediates the targeting of proteins for degradation. Mutations in the PARK2 gene cause a familial form of Parkinson’s disease called autosomal recessive juvenile parkinsonism (AR-JP).
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 2.0 - 10 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration of 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Human parkin is a 465 amino acid protein with a predicted molecular mass of ~52 kD.
Distribution
Tissue distribution: Brain, heart, testis, and skeletal muscle
Cellular distribution: Cytosol, mitochondria, nucleus, and endoplasmic reticulum
Function
Parkin is involved in protein ubiquitination, protein degradation, mitochondria function, and neurodegeneration.
Interaction
Ubiquitin, PINK1
Ligand/Receptor
BCL2, SYT11, CCNE1, GPR37, RHOT1/MIRO1, MFN1
Cell Type
Neurons
Biology Area
Cell Biology, Mitochondrial Function, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Protein Misfolding and Aggregation, Protein Trafficking and Clearance, Ubiquitin/Protein Degradation
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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