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- Clone
- 2A10 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PP2A-alpha, PPP2CA
- Previously
-
Covance Catalog# MMS-5117
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
N-terminally HA-tagged PPH21 was immunoprecipitated with 12CA5 anti-HA antibody (cross linked to protein A sepharose) from lysates of wtBY4741 (left) or PPM1δ (right) (lacking the PP2A methyltransferase) yeast cells, separated by 10% SDS-PAGE and blotted onto nitrocellulose membrane. Blots were incubated at 4°C o/n in a Miniblotter 45 (Immunetics) with 2A10 at the indicated dilutions. Anti-HA tag clone 16B12 served as positive control.
| Cat # | Size | Price | Save |
|---|---|---|---|
| 828801 | 100 µL | ¥73,700 |
PP2AC (Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform), encoded by the PPP2CA gene, is the main phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2AC is reversibly methyl esterified on Leu-309 by leucine carboxyl methyltransferase 1 (LCMT1) and protein phosphatase methylesterase 1 (PPME1). This carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, which in turn, modulates the PP2A holoenzyme's enzyme activity and cellular localization.
Product DetailsProduct Details
- Verified Reactivity
- Yeast
- Reported Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against a synthetic peptide from Human methylated Leucine 309 PP2A catalytic subunit.
- Formulation
- Phosphate-buffered solution.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/ml
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
-
WB
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting.
The optimal working dilution should be determined for each specific assay condition.
• WB: 1:100 - 1:800*
- Application Notes
-
This antibody is effective in immunoblotting (WB).
* Expected MW: 36 kD
WB Positive Control: BY4741 yeast cells
The immunogen sequence (C-ßA-RRTPDYFL-Ome) is conserved from human, mouse to yeast. - Product Citations
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- RRID
-
AB_2564923 (BioLegend Cat. No. 828801)
Antigen Details
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Gene ID
- 5515 View all products for this Gene ID
- UniProt
- View information about PP2AC Methyl Leu309 on UniProt.org
Other Formats
View All PP2AC Methyl (Leu309) Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-PP2AC Methyl (Leu 309) | 2A10 | WB |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-PP2AC Methyl (Leu 309)
N-terminally HA-tagged PPH21 was immunoprecipitated with 12C...
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