Recombinant Human IL-4 (carrier-free)

Product Details

Source

Human IL-4, amino acids His25-Ser153 (Accession# NM_000589) was expressed in E.coli.

Molecular Mass

The 130 amino acid recombinant protein has a predicted molecular mass of approximately 15.1 kD. The N-terminal amino acid is Met.

Purity

Purity is >95%, as determined by Coomassie stained SDS-PAGE.

Formulation

The protein was 0.22 µm filtered in PBS, pH 7.2.

Endotoxin Level

Less than 0.01ng per µg cytokine as determined by the LAL method

Preparation

For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 µg/mL in sterile buffer containing carrier protein such as 1% BSA or HSA or 10% FBS.

Concentration

10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling

Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Activity

ED₅₀ = 0.04 - 0.2 ng/mL as determined by the dose-dependent stimulation of TF-1 cell proliferation.

The specific activity of recombinant human IL-4 is approximately 1.02 x 10⁴ IU/µg when compared against the 1st WHO International Standard for Human Interleukin-4 (NIBSC code: 88/656) as determined by the dose-dependent stimulation of TF-1 cell proliferation.

For more information on specific activity, please visit the Recombinant Protein Unit Conversions page.

Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Application References

(PubMed link indicates BioLegend citation)

1. Chalubinski M, et al. 2014. Food Chem Toxicol. 69:289. PubMed

Product Citations

1. Duhen R, et al. 2022. J Clin Invest. 132: . PubMed
2. Chen WLK, et al. 2021. ACS Infect Dis. 7:838. PubMed
3. Zhang J, et al. 2023. Cell Death Dis. 737:14. PubMed
4. Zhang C, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
5. Coray M, et al. 2022. Int J Mol Sci. 23:. PubMed
6. Zhang H, et al. 2020. Front Cell Dev Biol. 8:205. PubMed
7. Moran MC, et al. 2022. JID Innov. :100151. PubMed
8. Baleeiro RB, et al. 2022. Oncoimmunology. 11:2080329. PubMed
9. Trapecar M, et al. 2021. Sci Adv. 7:00. PubMed
10. Guo J, et al. 2019. Cancer Immunol Res. 1.349305556. PubMed
11. Fosdick MG, et al. 2022. Sci Rep. 12:13506. PubMed
12. Wu W, et al. 2015. Am J Pathol. 185: 2324-2335. PubMed
13. Yamada KJ, et al. 2020. PLoS Pathog. 16:e1008354. PubMed
14. Yamaguchi Y, et al. 2022. J Immunother Cancer. 10:. PubMed
15. Wang LW, et al. 2019. Cell Metab. 30:539. PubMed
16. Brasil da Costa FH, et al. 2020. PLoS One. 15:e0230354. PubMed
17. Chalubinski M, et al. 2020. APMIS. 128:10. PubMed
18. Koh WH, et al. 2020. STAR Protoc. 1:100203. PubMed
19. Arora S, et al. 2021. Med (N Y). 2:938. PubMed
20. Chalubinski M, et al. 2014. Food Chem Toxicol. 69:289. PubMed

Antigen Details

Structure

Heterodimer

Distribution

IL-4 is produced by Th2 cells, naive CD4+ T cells, NKT cells, and basophils.

Function

IL-4 has a crucial role in the differentiation of TH2 cells and induction of Th2 associated cytokines. IL-4, through its activation of STAT6, upregulates GATA3 expression and also suppresses TH1 and TH17 cell responses, partly through the upregulation of growth factor independent 1(GFI1), a transcriptional repressor of IFNγ and IL-17 production. IL-4 induces macrophage activation and TSLP production. IL-4 recruits and activates IgE-producing B cells (IgE class switching) and enhances IgE-mediated responses by up-regulating IgE receptors on B lymphocytes, mast cells, and basophils. In addition, IL-4 also induces VCAM-1 on vascular endothelium and thus directs the migration of T lymphocytes, monocytes, basophils, and eosinophils to the inflammation site.

Interaction

T cells, B cells, macrophages, epithelial cells, smooth muscle cells, and bronchial fibroblasts.

Ligand/Receptor

IL-4 signals through Type I (IL-4Rα, γc) and Type II receptors (IL-4Rα, IL-13Rα1) complexes.

Cell Type

Embryonic Stem Cells, Hematopoietic stem and progenitors

Biology Area

Cell Biology, Immunology, Stem Cells

Molecular Family

Cytokines/Chemokines

Antigen References

1. Swain SL, et al. 1990. J. Immunol. 145:3796.
2. Hsieh CS, et al. 1992. P. Natl. Acad. Sci. USA 89:6065.
3. Allison-Lynn A, et al. 2006. J. Immunol. 176:7456.
4. Kato A, et al. 2007. J. Immunol. 179:1080.
5. LaPorte SL, et al. 2008. Cell 132:259.
6. Martinez FO, et al. 2009. Annu. Rev. Immunol. 27:451.

Gene ID

3565 View all products for this Gene ID

UniProt

View information about IL-4 on UniProt.org

Documentation

• Technical data sheet
• Certificate of Analysis
• Material Safety Data Sheet

Reviews

Related Pages & Pathways

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Certificate of Analysis