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- Clone
- 201A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CLEC4C, CLECSF11, CLECSF7, DLEC, HECL
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
CD303, also known as BDCA-2 and CLEC4C, is a 38 kD type II transmembrane glycoprotein. It is a member of the C-type lectin superfamily. CD303 is expressed by plasmacytoid dendritic cells (pDCs) and is involved in cell adhesion, signaling, and antigen capture and processing. Crosslinking of CD303 inhibits the production of IFN-α/β and TLR-9 induced pDCs maturation.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 550 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC
- Recommended Usage
-
Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. - Excitation Laser
-
Blue Laser (488 nm)
- Additional Product Notes
-
For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
-
Application References
(PubMed link indicates BioLegend citation) - RRID
-
AB_3106272 (BioLegend Cat. No. 285005)
Antigen Details
- Structure
- Type II transmembrane protein, 38 kD, member of the C-type lectin superfamily
- Distribution
-
Plasmacytoid dendritic cells
- Function
- Antigen capture, inhibition of IFN α/β production
- Cell Type
- Dendritic cells
- Biology Area
- Costimulatory Molecules, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Jähn PS, et al. 2010. Cell Immunol. 265:15.
2. Graham LM and Brown GD. 2009. Cytokine 48:148.
3. Röck J, et al. 2007. Eur. J. Immunol. 37:3564.
4. Dzionek A, et al. 2001. J. Exp. Med. 194:1823. - Gene ID
- 170482 View all products for this Gene ID
- UniProt
- View information about CD303 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- What are Flexi-Fluors?
-
Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.
- How quickly will I receive my order?
-
We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.
- How are Flexi-Fluors different from regular catalog products?
-
Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.
- How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?
-
Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
- I can’t find the antibody-dye combination that I need. When will it be available?
-
We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.
- I need help to validate the performance of my Flexi-Fluor. Who should I contact?
-
Please get in touch with Technical Service for assistance.
- Can I order more than 50 μg of a Flexi-Fluor?
-
Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.
- What is the expiration date of my Flexi-Fluor?
-
Expiration dates can be found on the vial label or by using our CoA lookup tool.
Other Formats
View All CD303 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human CD303 (BDCA-2)
Human peripheral mononuclear cells were stained with CD123 P... 
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Purified anti-human CD303 (BDCA-2)
Human peripheral mononuclear cells were stained with purifie... 
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PE anti-human CD303 (BDCA-2)
Human peripheral mononuclear cells were stained with CD123 A... 
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FITC anti-human CD303 (BDCA-2)
Human peripheral blood mononuclear cells were stained with C... 
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PerCP/Cyanine5.5 anti-human CD303 (BDCA-2)
Human peripheral mononuclear cells were stained with anti-hu... -
Brilliant Violet 421™ anti-human CD303 (BDCA-2)
Human peripheral mononuclear cells were stained with CD123 A... 
-
PE/Cyanine7 anti-human CD303 (BDCA-2)
Human peripheral mononuclear cells were stained with CD123 A... -
Purified anti-human CD303 (BDCA-2) (Maxpar® Ready)
Human PBMCs stained with 151Eu-anti-CD123 (6H6) and 153Eu-an... -
Alexa Fluor® 647 anti-human CD303 (BDCA-2)
Human peripheral mononuclear cells were stained with CD123 P... 
-
Biotin anti-human CD303 (BDCA-2)
Human peripheral mononuclear cells were stained with CD123 A... 
-
Brilliant Violet 785™ anti-human CD303 (BDCA-2)
Human peripheral mononuclear cells were stained with CD123 A... 
-
Brilliant Violet 605™ anti-human CD303 (BDCA-2)
Human peripheral mononuclear cells were stained with CD123 A... 
-
PE/Dazzle™ 594 anti-human CD303 (BDCA-2)
Human peripheral mononuclear cells were stained with CD123 A... 
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Alexa Fluor® 700 anti-human CD303 (BDCA-2)
Human peripheral mononuclear cells were stained with CD123 P... 
-
Alexa Fluor® 488 anti-human CD303 (BDCA-2)
RBC lysed human blood was stained with CD123 APC and CD303 (... 
-
Brilliant Violet 510™ anti-human CD303 (BDCA-2)
Human peripheral blood lymphocytes were stained with CD123 A... -
Brilliant Violet 711™ anti-human CD303 (BDCA-2)
Human peripheral blood lymphocytes were stained with CD123 P... -
APC/Fire™ 750 anti-human CD303 (BDCA-2)
Human peripheral blood lymphocytes and monocytes were stain... -
APC/Cyanine7 anti-human CD303 (BDCA-2)
Human peripheral mononuclear cells were stained with with Tr... -
TotalSeq™-A0370 anti-human CD303 (BDCA-2)
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TotalSeq™-C0370 anti-human CD303 (BDCA-2)
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TotalSeq™-B0370 anti-human CD303 (BDCA-2)
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TotalSeq™-D0370 anti-human CD303 (BDCA-2)
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Spark Blue™ 550 anti-human CD303 (BDCA-2) (Flexi-Fluor™)
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Spark Red™ 718 anti-human CD303 (BDCA-2) (Flexi-Fluor™)
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APC/Fire™ 810 anti-human CD303 (BDCA-2)
Human peripheral mononuclear cells were stained with anti-hu... -
Spark Violet™ 538 anti-human CD303 (BDCA-2)
Human peripheral mononuclear cells were stained with anti-hu... -
PerCP/Fire™ 806 anti-human CD303 (BDCA-2)
Human peripheral mononuclear cells were stained with anti-hu...
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