Spark Blue™ 574 anti-mouse/human CD11b (Flexi-Fluor™) Antibody, CD11b, M1/70

Clone: M1/70 (See other available formats)
Regulatory Status: RUO
Other Names: αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR, ITGAM
Isotype: Rat IgG2b, κ
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Product Citations: publications

Compare all formats See Spark Blue™ 574 spectral data

Cat #	Size	Price	Save
285022	50 µg	¥73,920

Description

CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse, Human, Cynomolgus, Rhesus
Reported Reactivity: Chimpanzee, Baboon, Rabbit
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: C57BL/10 splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 574 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC
Recommended Usage: Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone M1/70 has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F).

Additional reported applications (for relevant formats of this clone) include: immunoprecipitation^1,4, in vitro blocking^3,9,12, depletion^2,8, immunofluorescence microscopy^6,7,10, immunohistochemistry of acetone-fixed frozen sections^5,11-13, and spatial biology (IBEX)^35,36. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 101248).
Additional Product Notes: For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
RRID: AB_3106287 (BioLegend Cat. No. 285022)

Antigen Details

Structure: Integrin family, associates with integrin β₂ (CD18), 170 kD
Distribution: Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subsets of T and B cells
Function: Adhesion, chemotaxis
Ligand/Receptor: ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, fibrinogen
Cell Type: B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Coxon A, et al. 1996. Immunity 5:653.
Gene ID: 16409 View all products for this Gene ID 3684 View all products for this Gene ID
UniProt: View information about CD11b on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

What are Flexi-Fluors?

Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.

How quickly will I receive my order?

We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.

How are Flexi-Fluors different from regular catalog products?

Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.

How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?

Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

I can’t find the antibody-dye combination that I need. When will it be available?

We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.

I need help to validate the performance of my Flexi-Fluor. Who should I contact?

Please get in touch with Technical Service for assistance.

Can I order more than 50 μg of a Flexi-Fluor?

Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.

What is the expiration date of my Flexi-Fluor?

Expiration dates can be found on the vial label or by using our CoA lookup tool.

Other Formats

View All CD11b Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-mouse/human CD11b	M1/70	FC
Biotin anti-mouse/human CD11b	M1/70	FC
FITC anti-mouse/human CD11b	M1/70	FC
PE anti-mouse/human CD11b	M1/70	FC,IHC
PE/Cyanine5 anti-mouse/human CD11b	M1/70	FC
Purified anti-mouse/human CD11b	M1/70	FC,CyTOF®,IP,IHC-F,ICC
PE/Cyanine7 anti-mouse/human CD11b	M1/70	FC
Alexa Fluor® 488 anti-mouse/human CD11b	M1/70	FC,IHC-F,3D IHC,SB
Alexa Fluor® 647 anti-mouse/human CD11b	M1/70	FC,3D IHC,SB
Alexa Fluor® 700 anti-mouse/human CD11b	M1/70	FC
Pacific Blue™ anti-mouse/human CD11b	M1/70	FC
APC/Cyanine7 anti-mouse/human CD11b	M1/70	FC
PerCP/Cyanine5.5 anti-mouse/human CD11b	M1/70	FC
PerCP anti-mouse/human CD11b	M1/70	FC
Brilliant Violet 421™ anti-mouse/human CD11b	M1/70	FC
Brilliant Violet 570™ anti-mouse/human CD11b	M1/70	FC
Brilliant Violet 605™ anti-mouse/human CD11b	M1/70	FC
Brilliant Violet 650™ anti-mouse/human CD11b	M1/70	FC
Brilliant Violet 711™ anti-mouse/human CD11b	M1/70	FC
Brilliant Violet 785™ anti-mouse/human CD11b	M1/70	FC
Brilliant Violet 510™ anti-mouse/human CD11b	M1/70	FC,ICC
Ultra-LEAF™ Purified anti-mouse/human CD11b	M1/70	FC,CyTOF®,IP,Block,Depletion,IHC-F,ICC
Purified anti-mouse/human CD11b (Maxpar® Ready)	M1/70	FC,CyTOF®
Alexa Fluor® 594 anti-mouse/human CD11b	M1/70	IHC-F,FC
PE/Dazzle™ 594 anti-mouse/human CD11b	M1/70	FC
APC/Fire™ 750 anti-mouse/human CD11b	M1/70	FC
TotalSeq™-A0014 anti-mouse/human CD11b	M1/70	PG
Brilliant Violet 750™ anti-mouse/human CD11b	M1/70	FC
TotalSeq™-B0014 anti-mouse/human CD11b	M1/70	PG
TotalSeq™-C0014 anti-mouse/human CD11b	M1/70	PG
Spark NIR™ 685 anti-mouse/human CD11b	M1/70	FC
PE/Fire™ 640 anti-mouse/human CD11b	M1/70	FC
Spark YG™ 593 anti-mouse/human CD11b	M1/70	FC
Spark YG™ 570 anti-mouse/human CD11b	M1/70	IHC-F
PE/Fire™ 810 anti-mouse/human CD11b	M1/70	FC
APC/Fire™ 810 anti-mouse/human CD11b Antibody	M1/70	FC
Spark Blue™ 550 anti-mouse/human CD11b	M1/70	FC
Spark UV™ 387 anti-mouse/human CD11b	M1/70	FC
PerCP/Fire™ 806 anti-mouse/human CD11b	M1/70	FC
PerCP/Fire™ 780 anti-mouse/human CD11b	M1/70	FC
Spark Blue™ 574 anti-mouse/human CD11b (Flexi-Fluor™)	M1/70	FC
KIRAVIA Blue 520™ anti-mouse/human CD11b	M1/70	FC
PE/Fire™ 744 anti-mouse/human CD11b	M1/70	FC
Spark PLUS UV395™ anti-mouse/human CD11b	M1/70	FC
Spark Red™ 718 anti-mouse/human CD11b (Flexi-Fluor™)	M1/70	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 02/06/2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.