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Spark Violet™ 538 anti-human CD8 Recombinant Antibody

Pricing & Availability
Clone
QA18A37 (See other available formats)
Regulatory Status
RUO
Other Names
T8, Leu2
Isotype
Mouse IgG1, κ
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Product Citations
publications
QA18A37_SPKVL538_CD8_Antibody_121520
Human peripheral blood lymphocytes were stained with CD3 APC and CD8 recombinant (clone QA18A37) Spark Violet™ 538 (left) or mouse IgG1, k Spark Violet™ 538 isotype control (right).
  • QA18A37_SPKVL538_CD8_Antibody_121520
    Human peripheral blood lymphocytes were stained with CD3 APC and CD8 recombinant (clone QA18A37) Spark Violet™ 538 (left) or mouse IgG1, k Spark Violet™ 538 isotype control (right).
Compare all formats See Spark Violet™ 538 spectral data
Cat # Size Price Save
303805 25 tests ¥38,720
303806 100 tests ¥66,000
Description

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
PROPRIETARY
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark Violet™ 538 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Violet™ 538 has a maximum excitation of 396 nm and a maximum emission of 538 nm.

Excitation Laser
Violet Laser (405 nm)
RRID
AB_2890736 (BioLegend Cat. No. 303805)
AB_2890736 (BioLegend Cat. No. 303806)

Antigen Details

Structure
reIg superfamily, homodimer or heterodimer with CD8b, 32-34 kD
Distribution

Majority of thymocytes, T cell subset, NK cells

Ligand/Receptor
MHC Class I molecules
Cell Type
NK cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.

Gene ID
925 View all products for this Gene ID
UniProt
View information about CD8 on UniProt.org
Go To Top Version: 1    Revision Date: 12/15/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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