Loading...

True-Nuclear™ Transcription Factor Buffer Set

Pricing & Availability
Regulatory Status
RUO
Other Names
Transcription Factor Fixation/Permeabilization Buffer Set
Ave. Rating
Submit a Review
Product Citations
publications
Transcription_Factor_F_P_Buffer_1_ICFC_012915
Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with T-bet (clone 4B10) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • Transcription_Factor_F_P_Buffer_1_ICFC_012915
    Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with T-bet (clone 4B10) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • Transcription_Factor_F_P_Buffer_2_ICFC_012915
Cat # Size Price Save
424401 120 tests ¥36,300
Description

BioLegend's True-Nuclear™ Transcription Factor Buffer Set has been specially formulated for intracellular staining with minimum effect on the surface fluorochrome staining.

See the advantages of True-Nuclear™ Transcription Factor Buffer Set in this brief webinar: biolegend.com/true_nuclear_webinar.


 

Caution: The True-Nuclear™ Transcription Factor Buffer Set contains paraformaldehyde, which is toxic and mutagenic. Please handle with caution. Wear gloves, lab coats, and necessary protection to avoid direct contact. 

Product Details
Technical data sheet

Product Details

Storage & Handling
Store between 2°C and 8°C. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Prepare fresh Transcription Factor Fix working solution by diluting the 4X Fix Concentrate (1 part) with the Fix Diluent (3 parts). 1 mL of the 1X Fix working solution is needed for each tube.

Prepare a 1X working solution of the Perm Buffer by diluting the 10X Perm Buffer with distilled water. 6.5 mL of 1X Perm Buffer is needed for each sample of tube.

NOTE: The 10X Perm Buffer may have crystallization or precipitation when it is stored at 2-8°C; however, this is normal and does not affect the buffer's performance. If there is a heavy precipitation observed after dilution to 1X working solution, it can be filtered to clarify the solution.

Application Notes

Buffer Set Contents:

  • True-Nuclear™ 4X Fix Concentrate (30 mL)
  • True-Nuclear™ Fix Diluent (100 mL)
  • True-Nuclear™ 10X Perm Buffer (100 mL)

 

Additional Product Notes

View more applications data using this product to stain Veri-Cells™ lyophilized control cells, to detect transcription factors, and to study TIGIT in our Scientific Poster Library.

Product Citations
  1. Ajith A, et al. 2021. Front Immunol. 12:687715. PubMed
  2. Manso BA, et al. 2021. STAR Protoc. 2:100485. PubMed
  3. Wu HJ, et al. 2021. Nat Commun. 12:4897. PubMed
  4. Zeng N, et al. 2022. EMBO Rep. 23:e53302. PubMed
  5. Douglass SM, et al. 2021. Cancer Res. 81:658. PubMed
  6. Capelle CM, et al. 2022. Immunology. 165:428. PubMed
  7. Ellis GI, et al. 2022. Cell Rep Med. 3:100614. PubMed
  8. Grant NL, et al. 2022. Cell Rep. 39:110826. PubMed
  9. Hu M, et al. 2022. Int J Mol Sci. 23: . PubMed
  10. Jou E, et al. 2022. Sci Immunol. 7:eabn0175. PubMed
  11. Yu H, et al. 2022. Hematol Oncol. 40:894. PubMed
  12. Ogishi M, et al. 2023. J Exp Med. 220: . PubMed
  13. Isildar B, et al. 2022. Inflamm Regen. 42:55. PubMed
  14. Noman MZ, et al. 2022. Front Immunol. 13:980704. PubMed
  15. He Y, et al. 2022. Front Immunol. 13:986202. PubMed
  16. Ishii T, et al. 2022. Cell Death Discov. 8:480. PubMed
  17. Chen X, et al. 2022. J Extracell Vesicles. 11:e12279. PubMed
  18. Weng S, et al. 2022. Front Immunol. 13:1025931. PubMed
  19. Landshammer A, et al. 2023. Elife. 12: . PubMed
  20. Vyas M, et al. 2023. Front Immunol. 13:1098445. PubMed
  21. Wang L, et al. 2023. Oxid Med Cell Longev. 2023:6480848. PubMed
  22. Rustagi Y, et al. 2022. Diabetes. 71:1149. PubMed
  23. Zander R, et al. 2022. Immunity. 55:475. PubMed
  24. de Lemos L, et al. 2022. Development. 149:. PubMed
  25. Ogawa T, et al. 2020. J Immunol. 205:907. PubMed
  26. Wang L, et al. 2019. Front Pharmacol. 1.342361111. PubMed
  27. Shen M, et al. 2022. Adv Sci (Weinh). 9:e2203523. PubMed
  28. Cook ME, et al. 2022. Sci Immunol. 7:eabo0981. PubMed
  29. Whyte CE, et al. 2022. Curr Protoc. 2:e589. PubMed
  30. Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed
  31. Li Y, et al. 2023. Adv Mater. 35:e2208923. PubMed
  32. Schwarz A, et al. 2023. Front Immunol. 14:1038689. PubMed
  33. Kozlovski S, et al. 2023. Front Immunol. 13:1041552. PubMed
  34. Yi X, et al. 2023. Signal Transduct Target Ther. 8:107. PubMed
  35. Martins Nascentes Melo L, et al. 2023. J Immunother Cancer. 11:. PubMed
  36. McKenzie C, et al. 2023. Mol Ther Nucleic Acids. 32:603. PubMed
  37. Wu C, et al. 2023. Cancer Metab. 11:7. PubMed
  38. Adu-Berchie K, et al. 2023. Nat Commun. 14:3546. PubMed
  39. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  40. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  41. Bujanover N, et al. 2021. Stem Cell Reports. 16:1884. PubMed
  42. Kim D, et al. 2021. Nanomicro Lett. 13:31. PubMed
  43. Zhang M, et al. 2020. Nat Commun. 4.707638889. PubMed
  44. Baram T, et al. 2021. Cells. 10:. PubMed
  45. Yang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  46. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  47. Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed
  48. Hu HJ, et al. 2020. Cell Death Dis. 1.168055556. PubMed
  49. Guo R, et al. 2020. Nat Microbiol. 0.938194444. PubMed
  50. van der Heiden M, et al. 2020. Scand J Immunol. e13005:. PubMed
  51. Fronik P, et al. 2021. J Med Chem. 64:12132. PubMed
  52. Webster MR, et al. 2020. Mol Cell. 77:633. PubMed
  53. Lin H, et al. 2020. Front Microbiol. 1.330555556. PubMed
  54. Helm M, et al. 2022. Life (Basel). 12:. PubMed
  55. Woodruff MC, et al. 2020. Nat Immunol. 1.920833333. PubMed
  56. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  57. Cortez JT, et al. 2020. Nature. 582:416. PubMed
  58. Prodinger J, et al. 2016. J Leukoc Biol. 99: 583-594. PubMed
  59. Noman MZ, et al. 2020. Sci Adv. 6:eaax7881. PubMed
  60. Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed
  61. Guo W, et al. 2022. J Immunother Cancer. 10:. PubMed
  62. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  63. Ng W, et al. 2021. Pharm Biol. 59:47:00. PubMed
  64. Tian X, et al. 2021. Annals of Translational Medicine. 9(9):781. PubMed
  65. Wei SC, et al. 2020. Cancer Discov. . PubMed
  66. Qi X, et al. 2019. Nat Med. 25:1225. PubMed
  67. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  68. Freitas JT, et al. 2021. Pigment Cell Melanoma Res. 34:1084. PubMed
  69. Adamik J, et al. 2022. Nat Commun. 13:5184. PubMed
  70. He X, et al. 2021. Adv Sci (Weinh). 8:e2103023. PubMed
  71. Shevyrev D, et al. 2021. Exp Ther Med. 209:21. PubMed
  72. Synn CB, et al. 2022. Clin Transl Immunology. 11:e1364. PubMed
  73. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  74. Swartz AM, et al. 2022. Curr Protoc. 2:e410. PubMed
  75. Shiao SL, et al. 2021. Cancer Cell. 39:1202. PubMed
  76. Jarjour NN, et al. 2019. Nat Immunol. 1.310416667. PubMed
  77. de Andrade LF, et al. 2019. JCI Insight. 4:e133103. PubMed
  78. Pucella JN, et al. 2019. J Immunol. 202:1383. PubMed
  79. Goldfarb Y, et al. 2021. J Exp Med. 218:. PubMed
  80. Woodruff M, et al. 2020. medRxiv. . PubMed
  81. Wiarda JE, et al. 2022. Life Sci Alliance. 5:. PubMed
  82. Ellis GI, et al. 2022. STAR Protoc. 3:101784. PubMed
  83. Lü X, et al. 2021. Exp Ther Med. 22:1005. PubMed
  84. Li Y, et al. 2021. Cell Death Dis. 12:1001. PubMed
  85. Hu HJ, et al. 2021. Cancer Immunol Immunother. 70:3541. PubMed
  86. Ye Z, et al. 2021. Nat Commun. 12:907. PubMed
  87. Qiao J, et al. 2019. Cancer Cell. 35:901. PubMed
  88. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  89. Lundtoft C, et al. 2017. PLoS Pathogens. 13(6):e1006425. PubMed
  90. Kleiman E, et al. 2021. Sci Rep. 11:9561. PubMed
  91. Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
  92. Mudd PA, et al. 2022. Cell. 185:603. PubMed
  93. Zhang R, et al. 2022. Front Pharmacol. 13:870848. PubMed
  94. Rasmussen TA, et al. 2022. Cell Rep Med. 3:100766. PubMed
  95. Bin L, et al. 2017. APMIS. 10.1111/apm.12748. PubMed
  96. Saito E, et al. 2019. Biomaterials. 222:119432. PubMed
  97. Mirlekar B, et al. 2022. Cell Rep Med. :100744. PubMed
  98. de Picciotto S, et al. 2022. Nat Commun. 13:3866. PubMed
  99. Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
  100. Ye Z, et al. 2021. NPJ Aging Mech Dis. 7:4. PubMed
  101. Xu L, et al. 2020. Nat Cell Biol. 1162:22. PubMed
  102. Wang G, et al. 2020. Nat Commun. 0.611111111. PubMed
  103. Minns D, et al. 2021. Nat Commun. 12:1285. PubMed
  104. Dhar P, et al. 2021. Commun Biol. 4:905. PubMed
  105. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  106. Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed
  107. Datlinger P, et al. 2017. Nat Methods. 10.1038/nmeth.4177. PubMed
  108. Jarjour NN, et al. 2020. J Immunol. 204:923. PubMed
  109. Oherle K, et al. 2020. Immunity. 275:52. PubMed
  110. Okuma A, et al. 2017. Nat Commun.. 10.1038/s41467-017-02281-x. PubMed
  111. Yao C, et al. 2018. Autophagy. 1.854861111. PubMed
  112. Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
  113. Capelle CM, et al. 2021. Immunohorizons. 5:711. PubMed
  114. Lone W, et al. 2021. Clin Cancer Res. 27:6039. PubMed
  115. Chen Y, et al. 2021. Nat Immunol. 22:996. PubMed
  116. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  117. Alhudaithi SS, et al. 2020. Mol Pharm. 17:4691. PubMed
  118. Zhou H, et al. 2021. Cell Death Discov. 7:332. PubMed
  119. Chen M, et al. 2019. Cell Stem Cell. 25:501. PubMed
  120. Chen C, et al. 2020. Front Cell Neurosci. 14:8. PubMed
  121. Feng Y, et al. 2019. Stem Cell Res Ther. 0.479166667. PubMed
  122. Wang YJ, et al. 2021. Sci Rep. 11:8054. PubMed
  123. Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed
  124. Zhong C, et al. 2021. J Virol. 95:e0092521. PubMed
  125. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  126. Andricovich J, et al. 2016. J Clin Invest. 126:905-20. PubMed
  127. Yang X, et al. 2021. Bioact Mater. 3150:6. PubMed
  128. Zhu P, et al. 2022. Cell Commun Signal. 20:121. PubMed
  129. Zhao J, et al. 2021. Front Oncol. 10:621092. PubMed
  130. Prosser A, et al. 2021. STAR Protoc. 2:100810. PubMed
  131. Liu X, et al. 2021. Adv Sci (Weinh). 8:e2100233. PubMed
  132. Gonzalez MM, et al. 2021. J Clin Invest. 131:. PubMed
  133. Moritsch S, et al. 2022. Oncoimmunology. 11:2127271. PubMed
  134. Wang H, et al. 2022. J Cancer. 13:2126. PubMed
  135. Zhang L, et al. 2021. Mol Ther. 29:744. PubMed
  136. Vora AA, et al. 2021. STAR Protocols. 2(2):100565. PubMed
  137. Zeng Y, et al. 2019. FASEB J. 33:6596. PubMed

Antigen Details

Cell Type
Tregs
Biology Area
Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
NA
Go To Top Version: 5    Revision Date: 05/20/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account