Human peripheral blood mononuclear cells were either untreated (black) or treated with True-Stain Monocyte Blocker™ (red) and stained with CD14 (clone HCD14) Brilliant Violet™ 421 and CD19 (clone HIB19) PE/Dazzle™ 594 (left), CD3 (clone UCHT1) APC/Cyanine7 (middle), or CD56 (clone 5.1H11) PE/Cyanine5.
Human peripheral blood mononuclear cells were either untreated (black) or treated with True-Stain Monocyte Blocker™ (red) and stained with CD14 (clone HCD14) Brilliant Violet™ 421 and CD19 (clone HIB19) PE/Dazzle™ 594 (left), CD3 (clone UCHT1) APC/Cyanine7 (middle), or CD56 (clone 5.1H11) PE/Cyanine5.
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426101
25 tests
¥10,340
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426102
100 tests
¥31,020
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426103
500 tests
¥123,860
Description
True-Stain Monocyte Blocker™ has been formulated to block non-specific binding of PE/Dazzle™ 594, PE/Cyanine5, PE/Cyanine7, APC/Cyanine7, APC/Fire™ 750, and PE/Fire™ 744 commonly seen on monocytes and macrophages. The blocker is most effective when doing surface staining on live cells prior to the fixation step in a staining protocol. Learn more.
Add 5 µl of the True-Stain Monocyte Blocker™ to a suspension of cells immediately prior to the addition of antibodies, or mixed into the antibody cocktail, so that the final staining volume will equal 100 µl. This step can be performed at room temperature or on ice (2-8°C).
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