- Clone
- QA19A30 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD16, CD32
- Isotype
- Human IgG4, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Save |
---|---|---|---|
163403 | 50 µg | ¥14,300 | |
163404 | 500 µg | ¥60,720 |
CD16 is the low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. TruStain FcX™ PLUS (Human IgG4 anti-mouse CD16/32) is specific to the common epitope of CD16/CD32. It is useful for blocking non-specific binding of immunoglobulin to the Fc receptors and is more effective than TruStain FcX™.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Recombinant
- Host Species
- Human
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with TruStain FcX™ PLUS (Human IgG4 anti-mouse CD16/32) for 5 - 10 minutes, on ice, at 0.25 µg per 106 cells in a volume of 100 µL, prior to immunostaining. It is not necessary to wash the cells between the blocking and immunostaining steps.
- RRID
-
AB_2922498 (BioLegend Cat. No. 163403)
AB_2922498 (BioLegend Cat. No. 163404)
Antigen Details
- Structure
- Ig superfamily, 40-60 kD
- Distribution
-
B cells, monocyte/macrophages, NK cells, neutrophils, mast cells, dendritic cells
- Ligand/Receptor
- IgG
- Cell Type
- B cells, Dendritic cells, Macrophages, Mast cells, Monocytes, Neutrophils, NK cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Fc Receptors
- Gene ID
- 14130 View all products for this Gene ID 14131 View all products for this Gene ID
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
TruStain FcX™ PLUS (Human IgG4 anti-mouse CD16/32) Recombinant Antibody | QA19A30 | FC |
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TruStain FcX™ PLUS (Human IgG4 anti-mouse CD16/32) Recombinant Antibody
C57BL/6 splenocytes were incubated with TruStain FcX™ PLUS (...
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