- Clone
- J095G46 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Hexa histidine-tag, 6xHis-tag, 6XHis, 6His
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Save |
---|---|---|---|
362615 | 100 µg | ¥38,350 | |
362616 | 1 mg | ¥109,560 |
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
This antibody was raised against a linear 8XHis tag. It reacts with His-tagged protein with C, N or internal 6XHis tags or longer. It can be used for western blotting to detect linear His repeats (6XHis or more). It can also be used for ICFC and ICC staining of His-tagged proteins.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat, All species
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- KLH-conjugated His-tag peptide.
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
WB - Quality tested
ICC, FC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:1000-1:10000 diluted (0.05 - 0.5 µg/mL). For immunocytochemistry, a concentration range of 0.1 - 1.0 µg/mL (1:500 - 1:5000 dilution) is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody was raised against a linear 8X His tag. It reacts with proteins displaying repeats of 6 Histidines (His6) or more in either internal or C and N terminal regions. It is suitable for ICFC.
Clone J095G46 has been verified for immunocytochemistry (ICC). - RRID
-
AB_2814310 (BioLegend Cat. No. 362615)
AB_2814310 (BioLegend Cat. No. 362616)
Antigen Details
- Structure
- 6 repeat His amino acid
- Function
- Epitope tag
- Biology Area
- Cell Biology, Immunology
- Antigen References
-
- Zhao C, et al. 2010. Anal Biochem. 399:237
- Salichs E, et al. 2009. PLoS Genet. 5:e1000397
- Terpe K. 2003. Appl. Microbiol. Biotechnol. 60:523
- Lindner, P, et al. 1997. Biotechniques. 22:140
- Burritt JB, et al. 1995. J. Biol. Chem. 270:16974.
- Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.
- Gene ID
- NA
- UniProt
- View information about His Tag on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All His Tag Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
PE anti-His Tag | J095G46 | ICFC |
APC anti-His Tag | J095G46 | FC |
Alexa Fluor® 594 anti-His Tag | J095G46 | ICC |
Purified anti-His Tag | J095G46 | WB,ICC,FC |
Alexa Fluor® 647 anti-His Tag | J095G46 | ICC |
Direct-Blot™ HRP anti-His Tag | J095G46 | WB |
Alexa Fluor® 488 anti-His Tag | J095G46 | ICC |
Ultra-LEAF™ Purified anti-His Tag | J095G46 | WB,ICC,FC |
TotalSeq™-A1132 anti-His-Tag | J095G46 | PG |
TotalSeq™-B1132 anti-His-Tag | J095G46 | PG |
TotalSeq™-C1132 anti-His-Tag | J095G46 | PG |
PerCP/Cyanine5.5 anti-His-Tag | J095G46 | FC |
PE/Cyanine7 anti-His-Tag | J095G46 | FC |
FITC anti-His-Tag | J095G46 | FC |
Pacific Blue™ anti-His Tag | J095G46 | FC |
PE/Dazzle™ 594 anti-His-Tag | J095G46 | FC |
APC/Cyanine7 anti-His-Tag | J095G46 | FC |
PE/Cyanine5 anti-His-Tag | J095G46 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-His Tag
His-Tag transfected HEK293 cells were treated with Fixation ... His-Tag non-transfected HEK293 cells were treated with Fixat... -
APC anti-His Tag
CL hTMEM8A transfected P3.U1 stained with anti-His Tag (clon... -
Alexa Fluor® 594 anti-His Tag
HeLa cells transiently transfected with His Tag fused protei... HeLa cells transiently transfected with His Tag fused protei... -
Purified anti-His Tag
Total cell lysate (15 µg protein) from 293E (lane 1) and 293... HeLa cells transiently transfected with His tag fused protei... -
Alexa Fluor® 647 anti-His Tag
HeLa cells transiently transfected with His tag fused protei... HeLa cells transiently transfected with His tag fused protei... -
Direct-Blot™ HRP anti-His Tag
Whole cell lysates (15 µg protein) from HeLa cells transfect... -
Alexa Fluor® 488 anti-His Tag
HeLa cells transiently transfected with His tag fused protei... HeLa cells transiently transfected with His tag fused protei... -
Ultra-LEAF™ Purified anti-His Tag
Total cell lysate (15 µg protein) from 293E (lane 1) and 293... HeLa cells transiently transfected with His tag fused protei... -
TotalSeq™-A1132 anti-His-Tag
-
TotalSeq™-B1132 anti-His-Tag
-
TotalSeq™-C1132 anti-His-Tag
-
PerCP/Cyanine5.5 anti-His-Tag
Human TMEM8a (with His-Tag) transfected P3.U1 cells were sta... -
PE/Cyanine7 anti-His-Tag
Human TMEM8a (with His-Tag) transfected P3.U1 cells were sta... -
FITC anti-His-Tag
Human TMEM8a (with His-Tag) transfected P3.U1 cells were sta... -
Pacific Blue™ anti-His Tag
Human TMEM8A(with His Tag) transfected P3.U1 cells were stai... -
PE/Dazzle™ 594 anti-His-Tag
Human TMEM8a (with His-Tag) transfected P3.U1 cells were sta... -
APC/Cyanine7 anti-His-Tag
Human TMEM8a (with His-Tag) transfected P3.U1 cells were sta... -
PE/Cyanine5 anti-His-Tag
Human TMEM8a (with His-Tag) transfected P3.U1 cells were sta...
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