+ myeloid cells. In spleen, it is largely expressed on T cells.'>
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Alexa Fluor® 700 anti-mouse CD73 Antibody

Pricing & Availability
Clone
TY/11.8 (See other available formats)
Regulatory Status
RUO
Other Names
Ecto-5'-nucleotidase
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
TY-11dot8_A700_CD73_Antibody_022519
C57BL/6 mouse splenocytes were first treated with TruStain FcX™ (anti-mouse CD16/32) antibody and True-Stain Monocyte Blocker™. Cells were then stained with CD3ε PE and CD73 (clone TY/11.8) Alexa Fluor® 700 (left) or rat IgG1, κ Alexa Fluor® 700 isotype control (right).
  • TY-11dot8_A700_CD73_Antibody_022519
    C57BL/6 mouse splenocytes were first treated with TruStain FcX™ (anti-mouse CD16/32) antibody and True-Stain Monocyte Blocker™. Cells were then stained with CD3ε PE and CD73 (clone TY/11.8) Alexa Fluor® 700 (left) or rat IgG1, κ Alexa Fluor® 700 isotype control (right).
Compare all formats See Alexa Fluor® 700 spectral data
Cat # Size Price Save
127229 25 µg ¥33,660
127230 100 µg ¥64,460
Description

CD73 (ecto-5'-nucleotidase) is a 69 kD GPI-anchored surface protein. In mice, expression of CD73 in bone marrow is restricted to CD11b+ myeloid cells. In spleen, it is largely expressed on T cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CHO cells transfected with mouse CD73
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Yamshita Y, et al 1998. Eur. J. Immunol. 28:2981.
  2. Bentley JK, et al. 2010. Respir. Res. 11:127. (IHC-P)
Product Citations
  1. Bhattacharjee A, et al. 2021. Immunity. 54(8):1745-1757.e7. PubMed
RRID
AB_2800625 (BioLegend Cat. No. 127229)
AB_2800625 (BioLegend Cat. No. 127230)

Antigen Details

Structure
69 kD GPI-anchored surface protein
Distribution

Positive on most CD11b+ myeloid cells in bone marrow, but not on CD11b+ peripheral cells; a large proportion of CD4+ and CD8+ splenic T cells express CD73

Function
A nucleotidase catalyzing the dephosphorylation of extracellular nucleoside 5' monophosphates
Ligand/Receptor
Nucleoside 5' monophosphate
Cell Type
B cells, Mesenchymal Stem Cells, Tregs
Biology Area
Costimulatory Molecules, Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Yamashita Y, et al 1998. Eur. J. Immunol. 28:2981.
2. Resta R, et al. 1998. Immunol. Rev. 161:95.

Regulation
Expression is regulated by the development and maturation of leukocytes
Gene ID
23959 View all products for this Gene ID
UniProt
View information about CD73 on UniProt.org
Go To Top Version: 1    Revision Date: 02/25/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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