APC anti-mouse CD200R3 Antibody anti-CD200R3

Clone: Ba13 (See other available formats)
Regulatory Status: RUO
Other Names: CD200 R3, CD200RLb, OX-2 Receptor 3, CD200 receptor 3
Isotype: Rat IgG2a, κ
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Ba13_APC_CD200R3_Antibody_FC_1_101712 — C57BL/6 mouse peripheral blood leukocytes were stained with FcεRIα PE and CD200R3 (clone Ba13) APC (top) or rat IgG2a APC isotype control (bottom). Data shown were gated on myeloid cells population.

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Cat #	Size	Price	Save
142207	25 µg	¥28,820
142208	100 µg	¥74,360

Description

CD200R3, also known as CD200RLb and OX-2 Receptor 3, is a disulfide-linked dimeric CD200R-like receptor which belongs to immunoglobulin superfamily. Its positively charged amino acid lysine associates with ITAM- or YxxM motif-bearing adaptor molecules such as DAP12, DAP10, FcRγ, and CD3ζ. CD200R3 functions as an activating receptor to regulate IgE independent immune response.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse primary basophils
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Ba13 recognizes circulating and bone marrow basophils; it also recognizes a subset of mast cells in the peritoneal cavity and skin. Additional reported applications (for the relevant formats) include: stimulation of bone marrow derived basophils to produce IL-4.
RRID: AB_2561659 (BioLegend Cat. No. 142207)
AB_2561659 (BioLegend Cat. No. 142208)

Antigen Details

Structure: Immunoglobulin superfamily; disulfide-linked dimer with positively charge amino acids in transmembrane; molecular weight is about ~38 kD or ~74 kD (reduced or non-reduced conditions, respectively)
Distribution: Basophils in peripheral blood, bone marrow, spleen; mast cells in peritoneal cavity and skin
Function: Transduce stimulatory signals
Interaction: DAP12, DAP10, FcRγ, or CD3ζ
Cell Type: Basophils, Mast cells
Biology Area: Immunology, Inhibitory Molecules
Molecular Family: CD Molecules
Antigen References: 1. Voehringer D, et al. 2004. J. Biol. Chem. 52:54117.
2. Kojima T, et al. 2007. J. Immunol. 179:7093.
3. Sato K, et al. 2009. Blood 113:4780.
Gene ID: 74603 View all products for this Gene ID
UniProt: View information about CD200R3 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD200R3 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse CD200R3	Ba13	FC
PE anti-mouse CD200R3	Ba13	FC
APC anti-mouse CD200R3	Ba13	FC
TotalSeq™-A0809 anti-mouse CD200R3	Ba13	PG
PerCP/Cyanine5.5 anti-mouse CD200R3	Ba13	FC
PE/Cyanine7 anti-mouse CD200R3	Ba13	FC
Ultra-LEAF™ Purified anti-mouse CD200R3	Ba13	FC,Activ

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

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