Loading...

APC/Cyanine7 anti-mouse CD11c Antibody

Pricing & Availability
Clone
N418 (See other available formats)
Regulatory Status
RUO
Other Names
αX integrin, integrin αX chain, CR4, p150, ITGAX
Isotype
Armenian Hamster IgG
Ave. Rating
Submit a Review
Product Citations
publications
N418_APCCyanine7_CD11c_Antibody_022519
C57BL/6 mouse splenocytes were stained with I-A/I-E PE and CD11c (clone N418) APC/Cyanine7 (left) or Armenian hamster IgG APC/Cyanine7 isotype control (right).
  • N418_APCCyanine7_CD11c_Antibody_022519
    C57BL/6 mouse splenocytes were stained with I-A/I-E PE and CD11c (clone N418) APC/Cyanine7 (left) or Armenian hamster IgG APC/Cyanine7 isotype control (right).
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Save
117323 25 µg ¥16,740
117324 100 µg ¥51,980
Description

CD11c is a 150 kD glycoprotein also known as αX integrin, CR4, and p150. CD11c forms a αXβ2 heterodimer with β2 integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. The αXβ2 integrin plays an important role in cell-cell contact by binding its ligands: iC3b, fibrinogen, and CD54.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse spleen dendritic cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, immunofluorescence microscopy5, 9 (Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10), and spatial biology (IBEX)22,23.

Application References

(PubMed link indicates BioLegend citation)
  1. Granucci F, et al. 1997. J. Immunol. 159:1794.
  2. Stokes RW, et al. 1998. J. Immunol. 160:5514.
  3. Metlay JP, et al. 1990. J. Exp. Med. 171:1753. (IHC, IP)
  4. Ma XT, et al. 2006. Cancer Research 66:1169.
  5. Chin RK, et al. 2006. J. Immunol. 177:290. (IF)
  6. Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC) PubMed
  7. Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) PubMed
  8. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
  9. You Y, et al. 2009. J. Immunol. 182:7343. (IF) PubMed
  10. Roland CL, et al. 2009. Mol. Cancer Res. 8:1761. (IHC, FC) PubMed
  11. Wikstrom M, et al.2006. J. Immunol. 177:913. PubMed
  12. Pericolini E, et al. 2008. J. Leukocyte Biol. 83:1286. PubMed
  13. Randall LM, et al. 2008. Infect. Immun.76:3312. PubMed
  14. Fahlen-Yrild L, et al. 2009. J. Immunol. 183:5032. PubMed
  15. Osterholzer JJ, et al. 2009. J. Immunol. 183:8044. PubMed
  16. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  17. Eisenach PA, et al. 2010. J Cell Sci. 123:4182. PubMed
  18. Leppin K, et al. 2014. Invest. Ophthalmol. Vis. Sci. 55:3603. PubMed
  19. Sakai F, et al. 2014. PLoS One. 9:105370. PubMed
  20. Gibbins JD, et al. 2014. Blood. 124:2953. PubMed
  21. White CE, et al. 2015. J Immunol. 194:697. PubMed
  22. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  23. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  24. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Sasaki I, et al. 2012. Blood. 120:4733. PubMed
  2. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  3. Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed
  4. Sierro F, et al. 2017. Immunity. 47:374. PubMed
  5. Tomida S, et al. 2019. Sci Rep. 9:10751. PubMed
  6. Yu X, et al. 2020. Nat Commun. 11:1110. PubMed
  7. Heindl S, et al. 2021. J Exp Med. 218:. PubMed
  8. Liu H, et al. 2022. Cancer Immunol Res. 10:1028. PubMed
  9. Mansouri S, et al. 2022. J Immunol. 209:2114. PubMed
  10. Millar FR, et al. 2022. Cell Rep. 41:111596. PubMed
  11. Zhao M, et al. 2023. J Nanobiotechnology. 21:50. PubMed
  12. Sun Y, et al. 2023. Cell Mol Life Sci. 80:63. PubMed
  13. Xiong W, et al. 2022. Nat Commun. 13:1700. PubMed
  14. Deák P, et al. 2022. Cell Rep. 41:111563. PubMed
  15. Peng J, et al. 2023. Am J Reprod Immunol. 89:e13678. PubMed
  16. Clark JT, et al. 2023. Cell Rep. 42:112147. PubMed
  17. Wang YC, et al. 2023. J Exp Med. 220:. PubMed
  18. Li G, et al. 2023. Cell Death Dis. 14:185. PubMed
  19. Zhang J, et al. 2023. Theranostics. 13:2226. PubMed
  20. Hsieh MS, et al. 2023. NPJ Vaccines. 8:82. PubMed
  21. Zhang AQ, et al. 2023. Nat Biomed Eng. . PubMed
  22. Carone M, et al. 2023. Nat Commun. 14:3489. PubMed
  23. Herz J, et al. 2015. J Exp Med. 212: 1153 - 1169. PubMed
  24. Huang J, et al. 2022. J Transl Med. 20:496. PubMed
  25. Kitagawa S, et al. 2022. NPJ Vaccines. 7:115. PubMed
  26. Ortiz G, et al. 2020. Front Immunol. 11:1713. PubMed
  27. Lino AC et al. 2018. Immunity. 49(1):120-133 . PubMed
  28. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  29. Aldon Y, et al. 2020. J Immunol. 204:903. PubMed
  30. Devalaraja S, et al. 2020. Cell. 1098:180. PubMed
  31. Sari G, et al. 2020. Biochem Biophys Res Commun. 510:524. PubMed
  32. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
  33. Yoshioka D, et al. 2016. Antimicrob Agents Chemother. 60: 6146 - 6154. PubMed
  34. Shon W, et al. 2015. Sci Rep. 5:17305. PubMed
  35. Han Y, et al. 2020. Nat Commun. 11:1776. PubMed
  36. Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed
  37. Choi H et al. 2019. Cell Rep. 27(3):806-819 . PubMed
  38. Deslyper G, et al. 2021. Parasitology. 148:539. PubMed
  39. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  40. Casali P, et al. 2021. Front Immunol. 12:761450. PubMed
  41. Xu F, et al. 2022. Cell Death Discov. 8:142. PubMed
  42. Haugh KA, et al. 2021. eLife. 0.416666666666667. PubMed
  43. Sharma S, et al. 2015. J Immunol. 194:5529. PubMed
  44. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  45. Li Y, et al. 2022. Clin Transl Immunology. 11:e1362. PubMed
  46. Metzger D, et al. 2015. J Infect Dis. 212: 445-452. PubMed
  47. Dos Santos Dias L, et al. 2021. PLoS Pathog. e1009324:17. PubMed
  48. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  49. Devalaraja S, et al. 2020. STAR Protoc. 1:100188. PubMed
  50. Kölle J, et al. 2022. iScience. 25:104440. PubMed
  51. Salei N, et al. 2020. J Am Soc Nephrol. 31:257. PubMed
  52. Zhao F, et al. 2022. Front Immunol. 13:873720. PubMed
  53. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  54. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  55. Kotaki R, et al. 2020. Sci Rep. 10:13554. PubMed
  56. Trompette A, et al. 2022. Mucosal Immunol. :. PubMed
  57. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  58. Zhang K, et al. 2014. PLoS Pathog. 10:1004385. PubMed
  59. Furuya Y, et al. 2015. PLoS Pathog. 11: 1005180. PubMed
  60. Wessel AW, et al. 2020. Nat Commun. 4.123611111. PubMed
  61. Noah AC, et al. 2020. J Appl Physiol (1985). 473:128. PubMed
  62. Proto JD et al. 2018. Immunity. 49(4):666-677 . PubMed
  63. Friedman DJ, et al. 2021. Cancer Immunol Res. 9:952. PubMed
  64. Mulas F, et al. 2020. Cell Mol Immunol. . PubMed
  65. Viana C, et al. 2015. PLoS One. 10: 0130809. PubMed
  66. Davis M, et al. 2015. J Immunol. 194:2219. PubMed
  67. Suresh MV, et al. 2019. JCI Insight. 4:e133103. PubMed
  68. Takano T, et al. 2020. Front Immunol. 11:1555. PubMed
  69. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  70. Panea C, et al. 2021. Commun Biol. 4:913. PubMed
  71. Ariyoshi Y, et al. 2016. Gene Ther. 10.1038/gt.2016.7. PubMed
  72. Zeng W, et al. 2021. STAR Protocols. 2(1):100361. PubMed
  73. Branchett WJ, et al. 2020. Wellcome Open Research. 0.278472222. PubMed
  74. Zerif E, et al. 2020. Int J Mol Sci. 21:. PubMed
  75. Walsh SM, et al. 2021. eLife. 10:00. PubMed
  76. Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed
  77. Kim P, et al. 2015. Infect Immun . 83: 2992 - 3002. PubMed
  78. Ballet R, et al. 2014. PLoS Pathog. 10:1004550. PubMed
  79. Lin J, et al. 2021. Nat Commun. 12:5321. PubMed
  80. Galle-Treger L, et al. 2020. J Allergy Clin Immunol. 145:502. PubMed
  81. Shafiei-Jahani P, et al. 2021. Nat Commun. 12:2526. PubMed
  82. Alam Z, et al. 2020. Cell Rep. 107825:31. PubMed
  83. Echevarría-Vargas IM, et al. 2018. EMBO Mol Med. 10:e8446. PubMed
  84. Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed
  85. Zhang W, et al. 2020. Nat Commun. 11:1187. PubMed
  86. Nair S, et al. 2021. JCI Insight. 6:. PubMed
  87. Yang X, et al. 2022. Front Immunol. 13:856230. PubMed
  88. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  89. Sasaki Y, et al. 2015. PLoS One. 10: 0131176. PubMed
  90. Spangenberg E, et al. 2019. Nat Commun. 10:3758. PubMed
  91. Lee H, et al. 2017. J Immunol. 199:1453. PubMed
  92. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  93. Shibuya M, et al. 2021. iScience. 24:103131. PubMed
  94. Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed
  95. Guendel F, et al. 2020. Immunity. 53(5):1015-1032.e8. PubMed
  96. Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
  97. Draijer C, et al. 2018. Sci Rep. 8:5105. PubMed
  98. Wang T et al. 2018. Immunity. 49(3):504-514 . PubMed
  99. Darling R, et al. 2020. Int J Nanomedicine. 5.19375. PubMed
  100. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  101. Zhao N, et al. 2018. J Clin Invest. 26:84. PubMed
  102. Tulyeu J, et al. 2021. Curr Protoc. 1:e283. PubMed
  103. Zheng Y, et al. 2022. J Immunol. 208:501. PubMed
  104. Xiao Y, et al. 2021. Cell. 184:6037. PubMed
  105. Issah Y, et al. 2021. Elife. 10:. PubMed
  106. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  107. Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed
  108. Konda P, et al. 2022. Am J Cancer Res. 12:210. PubMed
  109. Ubags ND, et al. 2021. J Allergy Clin Immunol. 1049:147. PubMed
  110. Zhang L, et al. 2020. Cell. 442:181. PubMed
  111. Sheng J, et al. 2021. eLife. 10:00. PubMed
  112. Khan S, et al. 2016. J Exp Med. 213(13):2913-2929. PubMed
  113. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  114. Mogilenko DA et al. 2019. Cell. 177(5):1201-1216 . PubMed
  115. White JP et al. 2018. Cell. 175(5):1198-1212 . PubMed
  116. Lerbs T, et al. 2020. JCI Insight. 5:00. PubMed
  117. Zhu Y et al. 2017. The Journal of Neuroscience. 37(9):2362-2376 . PubMed
  118. Lau A, et al. 2022. Sci Adv. 8:eabm0142. PubMed
  119. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  120. Strait AA, et al. 2021. Commun Biol. 4:1005. PubMed
  121. Lucas ED, et al. 2020. Cell Reports. 33(2):108258. PubMed
  122. Choi J, et al. 2013. PLoS Negl Trop Dis. 7:e1981. PubMed
  123. Qi S, et al. 2020. Theranostics. 10:1814. PubMed
  124. Kim I et al. 2015. Brain and behavior. 5(12):e00403 . PubMed
  125. Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed
  126. Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
  127. Zhang SJ, et al. 2021. Adv Sci (Weinh). 8:2003410. PubMed
  128. Hohsfield LA, et al. 2021. Elife. 10:. PubMed
RRID
AB_830646 (BioLegend Cat. No. 117323)
AB_830646 (BioLegend Cat. No. 117324)

Antigen Details

Structure
Integrin α-chain, associates with integrin β2 (CD18), 150 kD
Distribution

Dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells

Function
Cellular adhesion
Ligand/Receptor
iC3b, fibrinogen
Cell Type
Dendritic cells, Epithelial cells, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lopez-Rodriguez C, et al. 1996. J. Immunol. 156:3780.

Gene ID
16411 View all products for this Gene ID
UniProt
View information about CD11c on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD11c Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD11c N418 FC
Biotin anti-mouse CD11c N418 FC,IHC-F
FITC anti-mouse CD11c N418 FC
PE anti-mouse CD11c N418 FC
Purified anti-mouse CD11c N418 FC,CyTOF®,IP,IHC
Alexa Fluor® 488 anti-mouse CD11c N418 FC
Alexa Fluor® 647 anti-mouse CD11c N418 FC,IHC,ICC,3D IHC
PE/Cyanine5 anti-mouse CD11c N418 FC
PE/Cyanine7 anti-mouse CD11c N418 FC
Brilliant Violet 605™ anti-mouse CD11c N418 FC
Alexa Fluor® 700 anti-mouse CD11c N418 FC
Pacific Blue™ anti-mouse CD11c N418 FC
APC/Cyanine7 anti-mouse CD11c N418 FC
PerCP/Cyanine5.5 anti-mouse CD11c N418 FC
PerCP anti-mouse CD11c N418 FC
Brilliant Violet 421™ anti-mouse CD11c N418 FC,IHC
Brilliant Violet 570™ anti-mouse CD11c N418 FC
Brilliant Violet 785™ anti-mouse CD11c N418 FC
Brilliant Violet 510™ anti-mouse CD11c N418 FC
Brilliant Violet 650™ anti-mouse CD11c N418 FC
Purified anti-mouse CD11c (Maxpar® Ready) N418 FC,CyTOF®
Alexa Fluor® 594 anti-mouse CD11c N418 IHC-F,3D IHC
PE/Dazzle™ 594 anti-mouse CD11c N418 FC
Brilliant Violet 711™ anti-mouse CD11c N418 FC
APC/Fire™ 750 anti-mouse CD11c N418 FC
TotalSeq™-A0106 anti-mouse CD11c N418 PG
Brilliant Violet 750™ anti-mouse CD11c N418 FC
TotalSeq™-B0106 anti-mouse CD11c N418 PG
TotalSeq™-C0106 anti-mouse CD11c N418 PG
KIRAVIA Blue 520™ anti-mouse CD11c N418 FC
Spark Blue™ 550 anti-mouse CD11c N418 FC
Spark NIR™ 685 anti-mouse CD11c N418 FC
Spark UV™ 387 anti-mouse CD11c N418 FC
Spark Red™ 718 anti-mouse CD11c N418 FC
Spark Blue™ 515 anti-mouse CD11c N418 FC
PerCP/Fire™ 806 anti-mouse CD11c N418 FC
Spark PLUS UV395™ anti-mouse CD11c N418 FC
Spark Blue™ 574 anti-mouse CD11c (Flexi-Fluor™) N418 FC
Go To Top Version: 2    Revision Date: 02/25/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account