Loading...

APC/Cyanine7 anti-mouse CD45 Antibody

Pricing & Availability
Clone
30-F11 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5, LCA
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
30-F11_APCCyanine7_CD45_Antibody_013019_updated.png
C57BL/6 splenocytes were stained with CD45 (clone 30-F11) APC/Cyanine7 (filled histogram) or Rat IgG2b, κ APC/Cyanine7 isotype control (open histogram).
  • 30-F11_APCCyanine7_CD45_Antibody_013019_updated.png
    C57BL/6 splenocytes were stained with CD45 (clone 30-F11) APC/Cyanine7 (filled histogram) or Rat IgG2b, κ APC/Cyanine7 isotype control (open histogram).
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Save
103115 25 µg ¥17,450
103116 100 µg ¥34,890
Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Chang YS, et al. 2020. Int J Mol Sci. 21:00. PubMed
  2. Naito H, et al. 2020. Nature Protocols. 15(3):1066-1081. PubMed
  3. Xing J, et al. 2021. Cell Reports. 35(12):109205. PubMed
  4. Heindl S, et al. 2021. J Exp Med. 218:. PubMed
  5. Stefkovich M, et al. 2021. Mol Metab. 54:101357. PubMed
  6. Wang Q, et al. 2020. Radiat Environ Biophys. 59:89. PubMed
  7. Kim MY, et al. 2021. JCI Insight. 6:. PubMed
  8. De Vries LCS, et al. 2021. J Crohns Colitis. 15:617. PubMed
  9. Bernasconi R, et al. 2021. EMBO Mol Med. :e14392. PubMed
  10. Miranda K, et al. 2022. iScience. 25:104994. PubMed
  11. Da Mesquita S, et al. 2021. Nature. 593:255. PubMed
  12. Furrer R, et al. 2021. Sci Adv. 7:eabi4852. PubMed
  13. Ren Y, et al. 2021. Am J Transplant. 21:552. PubMed
  14. Hilfenhaus G, et al. 2021. Cancer Res. 81:619. PubMed
  15. Bruno MEC, et al. 2022. J Cell Physiol. 237:706. PubMed
  16. Cohen K, et al. 2021. Cell Rep. 37:110026. PubMed
  17. Schnell A, et al. 2021. Cell. 184:6281. PubMed
  18. He D, et al. 2022. Immunity. 55:159. PubMed
  19. Alam A, et al. 2022. Cancer Cell. 40:153. PubMed
  20. Goncharov NV, et al. 2022. J Evol Biochem Physiol. 58:230. PubMed
  21. Li DD, et al. 2022. J Cell Mol Med. 26:2438. PubMed
  22. Denorme F, et al. 2022. J Clin Invest. 132: . PubMed
  23. Tang Q, et al. 2022. Mol Ther. 30:2709. PubMed
  24. Chae CS, et al. 2022. Cancer Discov. 12:1904. PubMed
  25. Miyao T, et al. 2022. Elife. 11: . PubMed
  26. Serra M, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:609. PubMed
  27. Poh AR, et al. 2022. Sci Adv. 8:eabl7882. PubMed
  28. Campisi L, et al. 2022. Nature. 606:945. PubMed
  29. Kenigsbuch M, et al. 2022. Nat Neurosci. 25:876. PubMed
  30. Zhao Z, et al. 2022. Adv Sci (Weinh). 9:e2201293. PubMed
  31. Li F, et al. 2022. Gut Microbes. 14:2096989. PubMed
  32. Rodrigues PB, et al. 2022. Cells. 11: . PubMed
  33. Zhu J, et al. 2022. Research (Wash D C). 2022:9816272. PubMed
  34. Poh AR, et al. 2022. Cell Rep. 41:111479. PubMed
  35. Zhu X, et al. 2023. Adv Mater. 35:e2207198. PubMed
  36. Li S, et al. 2022. Nat Commun. 13:7371. PubMed
  37. Morrow RJ, et al. 2022. Cells. 11: . PubMed
  38. Liu P, et al. 2023. Clin Immunol. 246:109212. PubMed
  39. Alula KM, et al. 2023. Sci Rep. 13:18. PubMed
  40. Peng Q, et al. 2023. EMBO Rep. 24:e56034. PubMed
  41. Montilla A, et al. 2023. Cell Death Dis. 14:16. PubMed
  42. Zhou J, et al. 2023. Nutrients. 15: . PubMed
  43. Slusarczyk P, et al. 2023. Elife. 12: . PubMed
  44. Cao Y, et al. 2023. J Inflamm Res. 16:343. PubMed
  45. Tsioti I, et al. 2023. J Neuroinflammation. 20:25. PubMed
  46. Li XF, et al. 2023. J Exp Med. 220: . PubMed
  47. Chun J, et al. 2023. Cancers (Basel). 15: . PubMed
  48. Drummer C, et al. 2023. Front Immunol. 14:1113883. PubMed
  49. Ishikawa T, et al. 2023. Bio Protoc. 13:e4588. PubMed
  50. Ryan CB, et al. 2022. Neurobiol Dis. 163:105608. PubMed
  51. Marsh SE, et al. 2022. Nat Neurosci. 25:306. PubMed
  52. Gomez-Salinero JM, et al. 2022. Cell Stem Cell. 29:593. PubMed
  53. Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed
  54. Ren D, et al. 2022. STAR Protoc. 3:101217. PubMed
  55. Korsunsky I, et al. 2022. Med. 3:481. PubMed
  56. Cheng C, et al. 2022. Cell Mol Gastroenterol Hepatol. 15:261. PubMed
  57. Pattwell SS, et al. 2022. Sci Adv. 8:eabo6789. PubMed
  58. Schroeter CB, et al. 2022. J Neuroinflammation. 19:270. PubMed
  59. Ho HM, et al. 2022. Pharmaceutics. 14:. PubMed
  60. Cai Z, et al. 2023. Adv Sci (Weinh). 10:e2207155. PubMed
  61. Banerjee K, et al. 2023. Eur J Immunol. 53:e2250161. PubMed
  62. Wu Z, et al. 2023. EMBO Rep. 24:e56524. PubMed
  63. Gao L, et al. 2023. J Nanobiotechnology. 21:56. PubMed
  64. Liu N, et al. 2023. Cell Death Dis. 14:170. PubMed
  65. Lu C, et al. 2023. Antiviral Res. 212:105556. PubMed
  66. Tang C, et al. 2023. Nat Commun. 14:1493. PubMed
  67. Saha S, et al. 2023. Sci Rep. 13:4609. PubMed
  68. Do-Thi VA, et al. 2023. Cancer Res Commun. 3:80. PubMed
  69. Freitag PC, et al. 2023. Mol Ther Methods Clin Dev. 29:120. PubMed
  70. Thai LM, et al. 2023. iScience. 26:106477. PubMed
  71. He Q, et al. 2023. Parasit Vectors. 16:139. PubMed
  72. Sasaki L, et al. 2022. Nat Aging. 2:105. PubMed
  73. Haensel D, et al. 2023. Nat Commun. 14:2685. PubMed
  74. Lee K, et al. 2023. JCI Insight. 8:. PubMed
  75. Wang C, et al. 2023. Int J Oral Sci. 15:19. PubMed
  76. van Os BW, et al. 2023. Front Cardiovasc Med. 10:1171764. PubMed
  77. Scieszka DP, et al. 2023. Respir Res. 24:138. PubMed
  78. Yang QC, et al. 2023. iScience. 26:106916. PubMed
  79. Ye S, et al. 2023. Front Oncol. 13:1202750. PubMed
  80. Cignarella F et al. 2018. Cell metabolism. 27(6):1222-1235 . PubMed
  81. Contijoch EJ et al. 2019. eLife. 8 pii: e40553. PubMed
  82. Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed
  83. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  84. Wang X, et al. 2019. Cell Res. 29:787. PubMed
  85. Yu X, et al. 2020. Nat Commun. 11:1110. PubMed
  86. Urban SK, et al. 2020. Liver Int. 40:3103. PubMed
  87. Kim JS, et al. 2020. Immunity. 54(1):176-190.e7. PubMed
  88. Rosenkranz SC, et al. 2021. eLife. 10:00. PubMed
  89. Kokona D, et al. 2016. Exp Eye Res. S0014-4835(16)30499-7. PubMed
  90. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  91. Wu SY, et al. 2022. Mol Cancer. 21:84. PubMed
  92. Effern M, et al. 2022. STAR Protoc. 3:101038. PubMed
  93. Jiang Q, et al. 2022. Theranostics. 12:59. PubMed
  94. Tripathi H, et al. 2020. J Mol Cell Cardiol. 149:95. PubMed
  95. Fan L, et al. 2021. Cancers (Basel). 13:. PubMed
  96. Page N, et al. 2018. Immunity. 48:937. PubMed
  97. Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed
  98. Ajina R, et al. 2019. Oncoimmunology. 8:e1577127. PubMed
  99. Groza D, et al. 2018. Oncoimmunology. 7:e1424676. PubMed
  100. Iwamoto H et al. 2018. Cell metabolism. 28(1):104-117 . PubMed
  101. Lyons J, et al. 2018. PLoS Biol. 16:e2002417. PubMed
  102. Radovanovic I, et al. 2014. J Immunol. 193:1290. PubMed
  103. Evans H, et al. 2015. J Immunol. 195: 2973 - 2984. PubMed
  104. Cardona S, et al. 2015. ASN Neuro. 7: 1759091415608204. PubMed
  105. Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
  106. Uzhachenko RV, et al. 2021. Cell Reports. 35(1):108944. PubMed
  107. Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed
  108. Van Den Eeckhout B, et al. 2020. NPJ Vaccines. 5:64. PubMed
  109. Baram T, et al. 2021. Cells. 10:. PubMed
  110. Pan Y, et al. 2021. Front Cell Neurosci. 15:664312. PubMed
  111. Yang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  112. Luo R, et al. 2021. Theranostics. 11:117. PubMed
  113. Adrover JM, et al. 2022. JCI Insight. 7:. PubMed
  114. Steubing RD, et al. 2022. Brain Behav Immun Health. 24:100493. PubMed
  115. Lu H, et al. 2021. Autophagy. 17:2511. PubMed
  116. Kawashima N, et al. 2022. Nat Commun. 13:1624. PubMed
  117. Zhu S, et al. 2022. Front Pharmacol. 13:996635. PubMed
  118. Palacios LM, et al. 2022. Biology (Basel). 11:. PubMed
  119. Ebneter A, et al. 2017. Transl Vis Sci Technol. 10.1167/tvst.6.2.10. PubMed
  120. Kim TJ, et al. 2019. Nat Commun. 10:3258. PubMed
  121. Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed
  122. Blecher-Gonen R, et al. 2019. Cell Syst. 8:109. PubMed
  123. Kashyap AS, et al. 2019. Cell Rep. 28:3367. PubMed
  124. Pluchino N, et al. 2020. J Cell Mol Med. 24:2464. PubMed
  125. Chou YJ, et al. 2020. Sci Rep. 10:8422. PubMed
  126. Forte E, et al. 2020. J Cell Mol Med. . PubMed
  127. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  128. Zhou X, et al. 2022. Front Pharmacol. 13:887263. PubMed
  129. Biswas S, et al. 2021. Nature. 591:464. PubMed
  130. Han Y, et al. 2020. Nat Commun. 11:1776. PubMed
  131. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  132. Haertel E, et al. 2018. Eur J Immunol. 48:1001. PubMed
  133. Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed
  134. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  135. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  136. Khandelwal P, et al. 2013. PLoS One. 8:64193. PubMed
  137. Shibata K, et al. 2014. J Immunol. 192:2210. PubMed
  138. Mantia-Smaldone G, et al. 2014. Gynecol Oncol. 133:584. PubMed
  139. Rybalko V, et al. 2015. PLoS One. 10: 0145550. PubMed
  140. AR P, et al. 2016. Circ Res. 118: 400-409. PubMed
  141. Zhu B, et al. 2021. Immunity. 54(6):1200-1218.e9. PubMed
  142. Zhao B, et al. 2021. Cell Reports. 36(3):109401. PubMed
  143. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  144. Uroda T, et al. 2020. Nat Protoc. 15:2107. PubMed
  145. Yoon Y, et al. 2021. Cancers (Basel). 13: . PubMed
  146. Shi S, et al. 2020. Clin Cancer Res. 26:5990. PubMed
  147. Mathur S, et al. 2021. JCI Insight. 6:. PubMed
  148. Armaka M, et al. 2022. Genome Med. 14:78. PubMed
  149. Yang W, et al. 2020. Mucosal Immunol. 13:788. PubMed
  150. Jung BG, et al. 2022. PLoS Pathog. 18:e1010454. PubMed
  151. Ganeshan K et al. 2019. Cell. 177(2):399-413 . PubMed
  152. Wiley CD, et al. 2019. JCI Insight. 4:e130056. PubMed
  153. Krasteva G, et al. 2011. Proc Natl Acad Sci U S A. 108:9478. PubMed
  154. Dieterich LC, et al. 2019. International Journal of Cancer. 145(10):2804-2815. PubMed
  155. Marangoni F, et al. 2021. Cell. . PubMed
  156. Wang LT, et al. 2021. STAR Protocols. 2(1):100337. PubMed
  157. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  158. Chew W, et al. 2016. Nat Methods. 13: 868-874. PubMed
  159. Cordeiro O, et al. 2016. PLoS One. 11: 0151848. PubMed
  160. Schönfeld M, et al. 2021. Alcohol Clin Exp Res. 45:1980. PubMed
  161. Bhattacherjee A, et al. 2021. Mol Neurodegener. 16:19. PubMed
  162. Bagayoko S, et al. 2021. PLoS Pathog. 17:e1009927. PubMed
  163. Zhang C, et al. 2020. Cell Metabolism. 31(1):148-161.e5.. PubMed
  164. Huang B, et al. 2020. Cell. 179(5):1160-1176.e24.. PubMed
  165. Ning F, et al. 2019. J Exp Med. 216:1396. PubMed
  166. Higashi AY, et al. 2019. J Am Soc Nephrol. 30:80. PubMed
  167. Lyons J, et al. 2018. Sci Signal. 11. PubMed
  168. Evans R, et al. 2019. Cell Rep. 27:1967. PubMed
  169. Hammond TR, et al. 2019. Immunity. 50:253. PubMed
  170. Liu M, et al. 2020. Cell Rep. 32:107990. PubMed
  171. Jeljeli M, et al. 2020. Cell Rep. 33:108325. PubMed
  172. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  173. Chi Z, et al. 2020. Molecular Cell. 80(1):43-58.e7. PubMed
  174. Gui J, et al. 2020. Nat Cancer. 1:603. PubMed
  175. Donlan AN, et al. 2020. Anaerobe. 66:102275. PubMed
  176. Uher O, et al. 2021. Cancers (Basel). 13:. PubMed
  177. Koliaraki V et al. 2019. Cell reports. 26(3):536-545 . PubMed
  178. Commerford CD et al. 2018. Cell reports. . PubMed
  179. Zeng J, et al. 2019. Cell Rep. 27:549. PubMed
  180. Yousef H, et al. 2018. Bio Protoc. 8:22. PubMed
  181. Willingham SB, et al. 2018. Cancer Immunol Res. 6:1136. PubMed
  182. Weiner AI, et al. 2019. NPJ Regen Med. 4:17. PubMed
  183. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  184. Qi X, et al. 2019. Nat Med. 25:1225. PubMed
  185. Liu H, et al. 2020. Cancer Cell. 37(3):324-339. PubMed
  186. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  187. Frii J, et al. 2021. Immunity. 54(5):1002-1021.e10. PubMed
  188. Alshetaiwi H, et al. 2020. Sci Immunol. 5:00. PubMed
  189. Zhang X, et al. 2020. Elife. 9:00. PubMed
  190. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  191. Mifflin L, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  192. Stewart JM, et al. 2020. ACS Biomater Sci Eng. 6:5941. PubMed
  193. Gao H, et al. 2021. Cells. 10:. PubMed
  194. Korangath P, et al. 2020. Bio Protoc. 10:e3822. PubMed
  195. Ajina R, et al. 2021. Cancer Immunol Res. 9:386. PubMed
  196. Utz B, et al. 2020. Sci Rep. 10:18733. PubMed
  197. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  198. Bahar Halpern K, et al. 2020. Nat Commun. 11:1936. PubMed
  199. Soldevilla MM, et al. 2019. Mol Ther. 27:1878. PubMed
  200. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  201. Li Y, et al. 2019. Sci Rep. 9:6932. PubMed
  202. Veremeyko T, et al. 2012. J Vis Exp. 65: 4097. PubMed
  203. Fu H, et al. 2020. Front Immunol. 11:595813. PubMed
  204. Oladiran O, et al. 2021. J Neuroinflammation. 77:18. PubMed
  205. Alikhanyan K, et al. 2021. Cancers (Basel). 13:. PubMed
  206. Du Y, et al. 2022. Nat Commun. 13:231. PubMed
  207. Ali M, et al. 2022. JCI Insight. 7:. PubMed
  208. Formaglio P, et al. 2021. Immunity. 54:2724. PubMed
  209. Cho YK, et al. 2022. Nat Commun. 13:4084. PubMed
  210. Ghandhi SA, et al. 2022. Sci Rep. 12:14124. PubMed
  211. Guo P, et al. 2021. J Immunol. 207:408. PubMed
  212. Ayaub E,et al. 2017. Sci Rep.. 10.1038/s41598-017-13511-z. PubMed
  213. Peralta Ramos JM, et al. 2017. Front Immunol. 1.490277778. PubMed
  214. Halpern KB, et al. 2018. Nat Biotechnol. 36:962. PubMed
  215. Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed
  216. McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
  217. Tacconi C, et al. 2021. Cell Reports. 35(2):108993. PubMed
  218. Dieterich LC, et al. 2020. Angiogenesis. 1.24375. PubMed
  219. Martina M, et al. 2014. J Vis Exp. 87: 51192. PubMed
  220. Hu Y, et al. 2022. J Nanobiotechnology. 20:417. PubMed
  221. Neuhöfer P, et al. 2021. Nature. 597:715. PubMed
  222. Yang J, et al. 2022. Microbiome. 10:149. PubMed
  223. Hum NR, et al. 2022. Front Oncol. 12:928474. PubMed
  224. Bergin SM, et al. 2021. Brain Behav Immun. 95:477. PubMed
  225. Selvaraj UM, et al. 2021. Brain Behav Immun. 95:502. PubMed
  226. Ying T, et al. 2021. Am J Physiol Endocrinol Metab. 320:E1148. PubMed
  227. Chen Y, et al. 2022. Front Immunol. 13:835690. PubMed
  228. Momin N, et al. 2022. Nat Commun. 13:109. PubMed
  229. Uddin MJ, et al. 2022. Mucosal Immunol. 15:165. PubMed
  230. Pilones KA, et al. 2020. Cancer Immunol Res. 8:1054. PubMed
  231. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  232. Chauveau A, et al. 2020. Immunity. 52:794. PubMed
  233. Haikala HM, et al. 2019. Nat Commun. 10:620. PubMed
  234. Abramowski P, et al. 2014. J Neuroimmunol. 274:111. PubMed
  235. Hoeksema M, et al. 2015. J Immunol. 194:3909. PubMed
  236. Dhanwani R, et al. 2020. Sci Adv. 6:eaba3688. PubMed
  237. Tripathi H, et al. 2020. Stem Cell Rev Rep. 0.953472222. PubMed
  238. Cardenas JJ, et al. 2020. Methods Enzymol. 415:631. PubMed
  239. Shannon JP, et al. 2021. STAR Protoc. 2:100790. PubMed
  240. Yang Y, et al. 2022. Elife. 11:. PubMed
  241. Alikhanyan K, et al. 2020. Immun Inflamm Dis. 8:181. PubMed
  242. Cheng Y, et al. 2019. Clin Cancer Res. 25:2621. PubMed
  243. Li H, et al. 2021. Adv Sci (Weinh). 2001596:8. PubMed
  244. Shannon JP, et al. 2021. Immunity. 54(2):276-290.e5. PubMed
  245. Alonso-Herranz L, et al. 2020. Elife. 9:00. PubMed
  246. Gierut J, et al. 2015. Stem Cell Res. 15: 165-171. PubMed
  247. Seki T, et al. 2015. J Biochem. 158: 485 - 495. PubMed
  248. Haka A, et al. 2012. PLoS One. 7:e44823. PubMed
  249. Wang R, et al. 2022. Front Immunol. 13:947756. PubMed
  250. Becker W, et al. 2021. J Crohns Colitis. 15:1032. PubMed
  251. Galle-Treger L, et al. 2020. J Allergy Clin Immunol. 145:502. PubMed
  252. Maulloo CD, et al. 2021. Front Immunol. 12:714842. PubMed
  253. Clement CC, et al. 2021. Nat Commun. 12:4447. PubMed
  254. Henrich IC, et al. 2021. Cancer Res. 81:2171. PubMed
  255. Kaya B, et al. 2020. Cell Reports. 32(5):107979. PubMed
  256. Sztwiertnia I, et al. 2020. PLoS One. 15:e0233789. PubMed
  257. Sydney Lavoie et al. 2019. eLife. 8 pii: e39982. PubMed
  258. Huetter J, et al. 2020. Cancer Immunol Res. 0.954861111. PubMed
  259. Favuzzi E, et al. 2021. Cell. 184(15):4048-4063.e32. PubMed
  260. DiToro D, et al. 2020. Immunity. 650:52. PubMed
  261. Wuggenig P, et al. 2020. Commun Biol. 3:130. PubMed
  262. Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
  263. Yu H, et al. 2021. Front Oncol. 11:736882. PubMed
  264. Zhang R, et al. 2022. Front Pharmacol. 13:870848. PubMed
  265. Yu W, et al. 2022. Nat Commun. 13:3544. PubMed
  266. Li D, et al. 2022. Emerg Microbes Infect. 11:2248. PubMed
  267. Veremeyko T, et al. 2019. J Neurosci Res. 97:162. PubMed
  268. Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed
  269. Kim MY, et al. 2018. Cell. 173:1439. PubMed
  270. Camara A, et al. 2019. Immunity. 50:1467. PubMed
  271. Michaelis KA, et al. 2019. Brain Behav Immun. 82:338. PubMed
  272. Ou YC, et al. 2020. ACS Nano. 1.035416667. PubMed
  273. Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
  274. Zelco A, et al. 2020. Front Cell Neurosci. 0.75625. PubMed
  275. Wang LT, et al. 2020. Cell Rep. 32:108188. PubMed
  276. Hadas R, et al. 2020. JCI Insight. 5:00. PubMed
  277. Stock M, et al. 2015. Osteoarthritis Cartilage. 23: 1221-1225. PubMed
  278. Hsu M, et al. 2022. Nat Immunol. 23:581. PubMed
  279. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
  280. Takaku S, et al. 2022. Evid Based Complement Alternat Med. 2022:6122955. PubMed
  281. Wang X, et al. 2022. Front Immunol. 13:864984. PubMed
  282. Galani IE, et al. 2022. STAR Protoc. 3:101151. PubMed
  283. Abdelfattah N, et al. 2022. Nat Commun. 13:767. PubMed
  284. Draper D, et al. 2012. J Immunol. 188:5327. PubMed
  285. Hohsfield LA, et al. 2020. J Neuroinflammation. 0.902083333. PubMed
  286. Godwin MS, et al. 2019. JCI Insight. 4:e126070. PubMed
  287. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  288. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  289. Selvaraj UM et al. 2018. Endocrinology. 159(11):3848-3859 . PubMed
  290. Pinho–Ribeiro FA, et al. 2018. Cell. 173:1083. PubMed
  291. Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed
  292. Martina M, et al. 2016. J Am Soc Nephrol. 27: 1113-1123. PubMed
  293. Da Mesquita S, et al. 2021. Science Advances. 7(21):. PubMed
  294. Bhatt D, et al. 2021. J Exp Med. 218:. PubMed
  295. Lu Z, et al. 2021. J Clin Invest. 131:. PubMed
  296. Luo M, et al. 2021. J Immunol. 207:1683. PubMed
  297. Wakabayashi T et al. 2018. Cell stem cell. 22(3):384-397 . PubMed
  298. Kaiser T, et al. 2019. Eneuro. 6. PubMed
  299. El Agha E, et al. 2017. Cell Stem Cell. 1.014583333. PubMed
  300. Yousef H, et al. 2019. Nat Med. 25:988. PubMed
  301. Ben-Moshe S, et al. 2019. Nat Metab. 1:899. PubMed
  302. Klein S, et al. 2016. J Cell Sci. 129: 2573 - 2585. PubMed
  303. Li N, et al. 2022. Nat Commun. 13:7281. PubMed
  304. Pedicone C, et al. 2022. iScience. 25:104170. PubMed
  305. Godwin MS, et al. 2021. Am J Physiol Lung Cell Mol Physiol. 320:L393. PubMed
  306. Reda M, et al. 2022. Nat Commun. 13:4261. PubMed
  307. Li Y, et al. 2022. STAR Protoc. 3:101364. PubMed
  308. Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
  309. Milich LM, et al. 2021. J Exp Med. 218:. PubMed
  310. Kline JM, et al. 2021. J Biol Chem. :100373. PubMed
  311. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  312. Starikova E, et al. 2021. Front Pharmacol. 12:667572. PubMed
  313. Yu L, et al. 2020. Nat Commun. 2.045833333. PubMed
  314. Liu X, et al. 2019. Mol Cancer Ther. 18:706. PubMed
  315. Ji L et al. 2019. Immunity. 51(2):272-284 . PubMed
  316. Jiang P, et al. 2018. Nat Med. 24:1550. PubMed
  317. Wang X, et al. 2018. Nat Commun. 9:551. PubMed
  318. Yoldi G, et al. 2016. Cancer Res . 76: 5857 - 5869. PubMed
  319. Ling Y, et al. 2016. Pharmacol Res. S1043-6618(16)31329-9. PubMed
  320. Blaszczak AM, et al. 2020. Immunometabolism. 2:00. PubMed
  321. Lu C, et al. 2020. Cancer Cell. 39(1):96-108.e6. PubMed
  322. Yang C, et al. 2020. Cell Host Microbe. 467:27. PubMed
  323. Morein D, et al. 2021. Cells. 10: . PubMed
  324. Yan C, et al. 2022. NPJ Precis Oncol. 6:6. PubMed
  325. Phansalkar R, et al. 2021. Elife. 10:. PubMed
  326. Kim S, et al. 2022. Cancers (Basel). 14:. PubMed
  327. Armaka M, et al. 2018. Nat Commun. 9:618. PubMed
  328. Lang JF et al. 2019. Nat Commun. 10(1):3415 . PubMed
  329. Theurich S et al. 2017. Cell metabolism. 26(1):171-184 . PubMed
  330. Martin A, et al. 2018. Oncotarget. 9:28364. PubMed
  331. Zhao Y, et al. 2020. Front Immunol. 2.572222222. PubMed
  332. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  333. Ireland L, et al. 2020. Front Immunol. 11:297. PubMed
  334. Gago-Lopez N, et al. 2019. EMBO Mol Med. 11:e10697. PubMed
  335. Van Den Eeckhout B, et al. 2020. NPJ Vaccines. 0.252777778. PubMed
  336. Uluçkan ö, et al. 2020. Cell Reports. 29(4):844-859.e3.. PubMed
  337. Liu SS, et al. 2021. Immunity. :. PubMed
  338. Ukidve A, et al. 2020. Proc Natl Acad Sci U S A. 117:17727. PubMed
  339. E O’Koren, R Mathew, D Saban 2016. Sci Rep. 6:20636. PubMed
  340. Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed
  341. Pelly V, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.4. PubMed
  342. Seeling M, et al. 2013. Proc Natl Acad Sci U S A. 110:10729. PubMed
  343. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
  344. Peng V, et al. 2022. Nat Immunol. 23:619. PubMed
  345. Wang Y, et al. 2022. Nat Commun. 13:1454. PubMed
  346. Lau P, et al. 2022. Cell Mol Immunol. :. PubMed
  347. Zhang X, et al. 2022. J Exp Clin Cancer Res. 41:246. PubMed
  348. Guan D, et al. 2021. Cell Death Dis. 12:431. PubMed
  349. Chen YL, et al. 2022. Front Neurosci. 16:876582. PubMed
  350. Xiao Z, et al. 2022. Mater Today Bio. 15:100297. PubMed
  351. Wang J, et al. 2021. Am J Cancer Res. 11:2005. PubMed
  352. Wang X, et al. 2021. Theranostics. 11:209. PubMed
  353. Willemze RA, et al. 2019. Mol Med. 25:01:00. PubMed
  354. Fransén Pettersson N, et al. 2018. PLoS One. 13:e0203228. PubMed
  355. Winter C, et al. 2018. Cell Metab. 28:175. PubMed
  356. Lux A, et al. 2014. Cell Rep. 10:236. PubMed
  357. Madsen P, et al. 2016. J Neurosci. 36: 5128 - 5143. PubMed
  358. Fox B, et al. 2016. PLoS One. 12: 1006189. PubMed
  359. Parker K, Horn L, Ostrand-Rosenberg S 2016. J Leukoc Biol. 100: 463 - 470.. PubMed
  360. Chen MB, et al. 2020. Cell Rep. 30:4418. PubMed
  361. Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
  362. Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
  363. Cong L, et al. 2021. Breast Cancer Res. 23:51. PubMed
  364. De Micheli AJ, et al. 2020. Am J Physiol Cell Physiol. 319:C885. PubMed
  365. Sibler E, et al. 2021. Cells. 10:. PubMed
  366. Oladiran O, et al. 2021. Front Immunol. 12:720733. PubMed
  367. Schäfer AL, et al. 2021. Front Immunol. 12:696810. PubMed
  368. Sebastian A, et al. 2021. Cells. 10:. PubMed
  369. Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed
  370. Petriello MC, et al. 2018. Toxicol Sci. 162:548. PubMed
  371. Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed
  372. Ioanna E Galani et al. 2017. Immunity. 46(5):875-890 . PubMed
  373. Forte E, et al. 2020. Cell Rep. 30:3149. PubMed
  374. Wang J, et al. 2020. J Hematol Oncol. 0.610416667. PubMed
  375. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  376. Parent AV, et al. 2021. Cell Reports. 36(7):109538. PubMed
  377. Ito Y, et al. 2021. Cell Reports. 35(4):109052. PubMed
  378. Ma Y, et al. 2021. Developmental Cell. 56(4):478-493.e11. PubMed
  379. Ye Y, et al. 2020. Genome Med. 0.557638889. PubMed
  380. Campisi L, et al. 2016. Nat Immunol. 10.1038/ni.3512. PubMed
  381. Holzapfel B, et al. 2015. Biomaterials. 61: 103-114. PubMed
  382. Lee S, et al. 2021. Autophagy. 18:1062. PubMed
  383. Swanson WB, et al. 2021. Biomaterials. 272:120769. PubMed
  384. Hohsfield LA, et al. 2021. Elife. 10:. PubMed
  385. Sun D, et al. 2020. PLoS Pathog. 16:e1008361. PubMed
  386. Zhu Q, et al. 2021. J Am Heart Assoc. 10:e023491. PubMed
  387. Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
  388. Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed
  389. Tong Y, et al. 2018. EBioMedicine. 39:132. PubMed
RRID
AB_312980 (BioLegend Cat. No. 103115)
AB_312980 (BioLegend Cat. No. 103116)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD45 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD45 30-F11 FC
Biotin anti-mouse CD45 30-F11 FC
FITC anti-mouse CD45 30-F11 FC
PE anti-mouse CD45 30-F11 FC
PE/Cyanine5 anti-mouse CD45 30-F11 FC
Purified anti-mouse CD45 30-F11 FC,IHC-F,CyTOF®,IP,CMCD,IHC,WB
PE/Cyanine7 anti-mouse CD45 30-F11 FC
APC/Cyanine7 anti-mouse CD45 30-F11 FC
Alexa Fluor® 488 anti-mouse CD45 30-F11 FC,SB
Alexa Fluor® 647 anti-mouse CD45 30-F11 FC,ICC,IHC,3D IHC,SB
Pacific Blue™ anti-mouse CD45 30-F11 FC
Alexa Fluor® 700 anti-mouse CD45 30-F11 FC,SB
PerCP/Cyanine5.5 anti-mouse CD45 30-F11 FC
PerCP anti-mouse CD45 30-F11 FC
Alexa Fluor® 594 anti-mouse CD45 30-F11 IHC-F,FC,3D IHC
Brilliant Violet 421™ anti-mouse CD45 30-F11 FC,SB
Brilliant Violet 570™ anti-mouse CD45 30-F11 FC
Brilliant Violet 510™ anti-mouse CD45 30-F11 FC
Brilliant Violet 605™ anti-mouse CD45 30-F11 FC
Purified anti-mouse CD45 (Maxpar® Ready) 30-F11 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45 30-F11 FC
Brilliant Violet 711™ anti-mouse CD45 30-F11 FC
Brilliant Violet 785™ anti-mouse CD45 30-F11 FC
Brilliant Violet 650™ anti-mouse CD45 30-F11 FC
APC/Fire™ 750 anti-mouse CD45 30-F11 FC
Brilliant Violet 750™ anti-mouse CD45 30-F11 FC
TotalSeq™-A0096 anti-mouse CD45 30-F11 PG
TotalSeq™-B0096 anti-mouse CD45 30-F11 PG
Ultra-LEAF™ Purified anti-mouse CD45 30-F11 FC,CyTOF®,IP,CMCD,IHC,WB
Spark Blue™ 550 anti-mouse CD45 30-F11 FC
Spark NIR™ 685 anti-mouse CD45 30-F11 FC
TotalSeq™-C0096 anti-mouse CD45 30-F11 PG
Spark YG™ 570 anti-mouse CD45 30-F11 IHC-F
PE/Fire™ 640 anti-mouse CD45 30-F11 FC
APC/Fire™ 810 anti-mouse CD45 30-F11 FC
PE/Fire™ 700 anti-mouse CD45 30-F11 FC
Spark Violet™ 538 anti-mouse CD45 30-F11 FC
Spark YG™ 593 anti-mouse CD45 30-F11 FC
Spark Blue™ 574 anti-mouse CD45 Antibody 30-F11 FC
Spark Blue™ 515 anti-mouse CD45 30-F11 FC
Spark UV™ 387 anti-mouse CD45 30-F11 FC
PE/Fire™ 810 anti-mouse CD45 30-F11 FC
Spark Red™ 718 anti-mouse CD45 (Flexi-Fluor™) 30-F11 FC
Spark PLUS UV395™ anti-mouse CD45 30-F11 FC
Go To Top Version: 3    Revision Date: 01/30/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account