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Brilliant Violet 605™ anti-mouse CD8a Antibody

Pricing & Availability
Clone
53-6.7 (See other available formats)
Regulatory Status
RUO
Other Names
T8, Lyt2, Ly-2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
53-6.7_BV605_072412
C57BL/6 mouse splenocytes were stained with CD3 FITC and CD8a (clone 53-6.7) BV605™.
  • 53-6.7_BV605_072412
    C57BL/6 mouse splenocytes were stained with CD3 FITC and CD8a (clone 53-6.7) BV605™.
Compare all formats See Brilliant Violet 605™ spectral data
Cat # Size Price Save
100743 125 µL ¥45,980
100744 50 µg ¥51,730
Description

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For immunofluorescent staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3, in vivo and in vitro cell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, immunohistochemical staining5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).

Application References

(PubMed link indicates BioLegend citation)
  1. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IHC, IP)
  2. Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete)
  3. Takahashi K, et al. 1992. P. Natl. Acad. Sci. USA 89:5557. (Block, IP)
  4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503. (Block)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Kamimura D, et al. 2006. J. Immunol. 177:306.
  9. Bouwer HGA, et al. 2006. P. Natl. Acad. Sci. USA 103:5102. (FC, Deplete)
  10. Kao C, et al. 2005. Int. Immunol. 17:1607. PubMed
  11. Ko SY, et al. 2005. J. Immunol. 175:3309. (FC) PubMed
  12. Rasmussen JW, et al. 2006. Infect. Immun. 74:6590. PubMed
  13. Lee CH, et al. 2009. Clin. Cancer Res. PubMed
  14. Geiben-Lynn R, et al. 2008. Blood 112:4585. (Deplete) PubMed
  15. Kingeter LM, et al. 2008. J. Immunol. 181:6244. PubMed
  16. Guo Y, et al. 2008. Blood 112:480. PubMed
  17. Andrews DM, et al. 2008. J. Virol. 82:4931. PubMed
  18. Britschqui MR, et al. 2008. J. Immunol. 181:7681. PubMed
  19. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  20. Jordan JM, et al. 2008. Infect. Immun. 76:3717. PubMed
  21. Todd DJ, et al. 2009. J. Exp. Med. 206:2151. PubMed
  22. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  23. Medyouf H, et al. 2010. Blood 115:1175. PubMed
  24. Riedl P, et al. 2009. J. Immunol. 183:370. PubMed
  25. Apte SH, et al. 2010. J. Immunol. 185:998. PubMed
  26. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  27. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  28. Cui L, et al. 2015. J Control Release. 206:220. PubMed
  29. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  30. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Schmid MC, et al. 2018. Nat Commun. 9:5379. PubMed
  2. Yu‐Han Chang et al. 2017. Immunity. 47(5):943-958 . PubMed
  3. Flamar AL, et al. 2020. Immunity. 52(4):606-619.e6.. PubMed
  4. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  5. Kim E, et al. 2022. Immunity. 55:145. PubMed
  6. Ning J, et al. 2022. Cancer Immunol Immunother. 71:1863. PubMed
  7. Paik D, et al. 2022. Nature. 603:907. PubMed
  8. Merana GR, et al. 2022. Cell Rep. 39:110891. PubMed
  9. Yang H, et al. 2023. Cell Death Differ. 30:560. PubMed
  10. Zhang B, et al. 2023. Signal Transduct Target Ther. 8:28. PubMed
  11. Wang J, et al. 2023. Inflamm Regen. 43:12. PubMed
  12. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  13. Ma Y, et al. 2022. Am J Physiol Heart Circ Physiol. 322:H622. PubMed
  14. Sheng YH, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:789. PubMed
  15. Konjar S, et al. 2022. Proc Natl Acad Sci U S A. 119:e2202144119. PubMed
  16. Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed
  17. Cai Z, et al. 2023. Adv Sci (Weinh). 10:e2207155. PubMed
  18. Lu C, et al. 2023. Antiviral Res. 212:105556. PubMed
  19. van Os BW, et al. 2023. Front Cardiovasc Med. 10:1171764. PubMed
  20. Park HJ, et al. 2023. NPJ Vaccines. 8:84. PubMed
  21. Wilden A, et al. 2022. Front Immunol. 13:991295. PubMed
  22. Denk D, et al. 2022. Immunity. 55:2059. PubMed
  23. Tata A, et al. 2021. Oncoimmunology. 10:1933808. PubMed
  24. Nato G, et al. 2021. Sci Rep. 11:651. PubMed
  25. Duong–Ly KC, et al. 2018. J Cell Sci. 131:17. PubMed
  26. Xu ZH, et al. 2021. Mediators Inflamm. 2021:8856326. PubMed
  27. Ma C, et al. 2022. Proc Natl Acad Sci U S A. 119:. PubMed
  28. Klarquist J, et al. 2021. Cell Rep. 36:109591. PubMed
  29. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  30. Zhang S, et al. 2021. Cell. 184(8):2151-2166.e16. PubMed
  31. Yousif AS, et al. 2020. Immunity. 54(2):235-246.e5. PubMed
  32. Bilate AM, et al. 2020. Immunity. 53(5):1001-1014.e20. PubMed
  33. Leech JM, et al. 2020. Cell Host & Microbe. 26(6):795-809.e5.. PubMed
  34. Huai W, et al. 2019. J Exp Med. 216:772. PubMed
  35. Piper CJM, et al. 2020. Cell Reports. 29(7):1878-1892.e7.. PubMed
  36. Crncec I, et al. 2018. Mol Oncol. 12:514. PubMed
  37. Grizotte–Lake M, et al. 2018. Immunity. 49:1103. PubMed
  38. Lutes LK, et al. 2021. eLife. 10:00. PubMed
  39. Qiu F, et al. 2022. J Cancer. 13:2893. PubMed
  40. Teng ZX, et al. 2022. J Inflamm Res. 15:3187. PubMed
  41. van der Veeken J et al. 2019. Immunity. 50(5):1202-1217 . PubMed
  42. Anderson CK et al. 2019. Cell Rep. 27(2):537-548 . PubMed
  43. Samarchith P Kurup et al. 2019. Cell host & microbe. 25(4):565-577 . PubMed
  44. Phiwpan K, et al. 2015. PLoS One. 10:128198. PubMed
  45. Chen L, et al. 2020. J Exp Med. 217:00:00. PubMed
  46. Zirngibl F, et al. 2021. J Immunother Cancer. 9:. PubMed
  47. Stewart JM, et al. 2020. ACS Biomater Sci Eng. 6:5941. PubMed
  48. Zhu XG, et al. 2020. Cell Metabolism. 33(1):211-221.e6. PubMed
  49. Tuganbaev T, et al. 2020. Cell. 182(6):1441-1459.e21. PubMed
  50. Aldrich AL, et al. 2021. The Journal of Immunology. 206(4):751-765. PubMed
  51. Miska J et al. 2019. Cell reports. 27(1):226-237 . PubMed
  52. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  53. Kim E, et al. 2022. STAR Protoc. 3:101366. PubMed
  54. Wennerberg E, et al. 2022. Sci Transl Med. 14:eabe8195. PubMed
  55. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  56. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  57. Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed
  58. Chauveau A, et al. 2020. Immunity. 52:794. PubMed
  59. Li Q, et al. 2019. Front Pharmacol. 10:786. PubMed
  60. Zhou AC, et al. 2019. J Immunol. 202:2482. PubMed
  61. Chauveau L, et al. 2021. EMBO Rep. 22:e52447. PubMed
  62. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  63. Shreeve N, et al. 2021. Immunity. 54(6):1231-1244.e4. PubMed
  64. Toshiro Hirai et al. 2019. Immunity. 50(5):1249-1261 . PubMed
  65. Olguín JE, et al. 2018. J Cancer. 9:239. PubMed
  66. Xiao S, et al. 2020. Cell Reports. 32(2):107892. PubMed
  67. Tummers B, et al. 2020. Immunity. 52(6):994-1006.e8. PubMed
  68. Giampazolias E, et al. 2021. Cell. . PubMed
  69. Robles-Oteiza C, et al. 2021. Dis Model Mech. 14:. PubMed
  70. Murray MP, et al. 2022. Cell Rep. 38:110209. PubMed
  71. Shibuya M, et al. 2021. iScience. 24:103131. PubMed
  72. Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed
  73. Emily A Thompson et al. 2019. Cell reports. 26(11):2859-2867 . PubMed
  74. Zhang X, et al. 2021. Nat Commun. 12:4536. PubMed
  75. Du P, et al. 2022. Front Pharmacol. 13:805508. PubMed
  76. Liu Y, et al. 2019. Sci Rep. 9:18970. PubMed
  77. Rosato PC, et al. 2019. Nat Commun. 10:567. PubMed
  78. Coley WD et al. 2018. F1000Research. 2018 Mar 14 [revised 2018 Jan 1] 0.5125. PubMed
  79. Scharschmidt TC, et al. 2017. Cell Host Microbe. 1.199305556. PubMed
  80. Hackstein CP, et al. 2022. Nat Commun. 13:7472. PubMed
  81. Dupraz L, et al. 2021. Cell Reports. 36(1):109332. PubMed
  82. Xu C, et al. 2021. Cell Reports. 35(11):109235. PubMed
  83. Pritykin Y, et al. 2021. Molecular Cell. 81(11):2477-2493.e10. PubMed
  84. Chen J, et al. 2014. Cell Res. 24:1050. PubMed
  85. Mathur AN, et al. 2019. Immunity. 50:655. PubMed
  86. Imbratta C, et al. 2019. Sci Rep. 9:6135. PubMed
  87. Young JS, et al. 2018. Front Immunol. 9:1385. PubMed
  88. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  89. Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed
  90. Riopel M, et al. 2019. Mol Metab. 20:89. PubMed
RRID
AB_2562609 (BioLegend Cat. No. 100743)
AB_2562609 (BioLegend Cat. No. 100744)

Antigen Details

Structure
Ig superfamily, CD8α chain, 34 kD
Distribution

Most thymocytes, T cell subset, some NK cells, lymphoid dendritic cells

Function
Co-receptor for TCR
Ligand/Receptor
MHC class I molecule
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Zamoyska R. 1994. Immunity 1:243.
3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.

Gene ID
12525 View all products for this Gene ID
UniProt
View information about CD8alpha on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD8a Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD8a 53-6.7 FC
Biotin anti-mouse CD8a 53-6.7 FC,IHC
FITC anti-mouse CD8a 53-6.7 FC
PE anti-mouse CD8a 53-6.7 FC
PE/Cyanine5 anti-mouse CD8a 53-6.7 FC
Purified anti-mouse CD8a 53-6.7 FC,CyTOF®,IHC-F,IP
PE/Cyanine7 anti-mouse CD8a 53-6.7 FC
APC/Cyanine7 anti-mouse CD8a 53-6.7 FC
Alexa Fluor® 488 anti-mouse CD8a 53-6.7 FC,3D IHC
Alexa Fluor® 647 anti-mouse CD8a 53-6.7 FC,IHC-F,3D IHC,SB
Pacific Blue™ anti-mouse CD8a 53-6.7 FC
Alexa Fluor® 700 anti-mouse CD8a 53-6.7 FC
PerCP/Cyanine5.5 anti-mouse CD8a 53-6.7 FC
PerCP anti-mouse CD8a 53-6.7 FC
Brilliant Violet 421™ anti-mouse CD8a 53-6.7 FC,IHC,SB
Brilliant Violet 570™ anti-mouse CD8a 53-6.7 FC
Brilliant Violet 650™ anti-mouse CD8a 53-6.7 FC
Brilliant Violet 605™ anti-mouse CD8a 53-6.7 FC
Ultra-LEAF™ Purified anti-mouse CD8a 53-6.7 FC,CyTOF®,IHC,IP,Depletion,Block
Brilliant Violet 711™ anti-mouse CD8a 53-6.7 FC
Brilliant Violet 785™ anti-mouse CD8a 53-6.7 FC
Brilliant Violet 510™ anti-mouse CD8a 53-6.7 FC
Purified anti-mouse CD8a (Maxpar® Ready) 53-6.7 FC,CyTOF®
Alexa Fluor® 594 anti-mouse CD8a 53-6.7 IHC-F,FC,3D IHC
PE/Dazzle™ 594 anti-mouse CD8a 53-6.7 FC
APC/Fire™ 750 anti-mouse CD8a 53-6.7 FC
GoInVivo™ Purified anti-mouse CD8a 53-6.7 FC
TotalSeq™-A0002 anti-mouse CD8a 53-6.7 PG
Spark Blue™ 550 anti-mouse CD8a 53-6.7 FC
Spark NIR™ 685 anti-mouse CD8a 53-6.7 FC
TotalSeq™-C0002 anti-mouse CD8a 53-6.7 PG
TotalSeq™-B0002 anti-mouse CD8a 53-6.7 PG
Spark YG™ 570 anti-mouse CD8a 53-6.7 IHC-F
PE/Fire™ 640 anti-mouse CD8a 53-6.7 FC
PE/Fire™ 700 anti-mouse CD8a 53-6.7 FC
Spark Blue™ 574 anti-mouse CD8a Antibody 53-6.7 FC
Spark Violet™ 423 anti-mouse CD8a Antibody 53-6.7 FC
Spark UV™ 387 anti-mouse CD8a 53-6.7 FC
Spark Blue™ 515 anti-mouse CD8a 53-6.7 FC
APC/Fire™ 810 anti-mouse CD8a 53-6.7 FC
Spark Red™ 718 anti-mouse CD8a (Flexi-Fluor™) 53-6.7 FC
PE/Fire™ 810 anti-mouse CD8a 53-6.7 FC
Spark PLUS UV395™ anti-mouse CD8a 53-6.7 FC
PerCP/Fire™ 780 anti-mouse CD8a 53-6.7 FC
Go To Top Version: 3    Revision Date: 01/21/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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