Loading...

FITC anti-mouse CD11c Antibody

Pricing & Availability
Clone
N418 (See other available formats)
Regulatory Status
RUO
Other Names
αX integrin, integrin αX chain, CR4, p150, ITGAX
Isotype
Armenian Hamster IgG
Ave. Rating
Submit a Review
Product Citations
publications
N418_A_FITC_Pos_071006
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and FITC N418 (top) or FITC Armenian hamster IgG isotype control (bottom).
  • N418_A_FITC_Pos_071006
    C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and FITC N418 (top) or FITC Armenian hamster IgG isotype control (bottom).
  • N418_B_FITC_Neg_071006
    C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and FITC N418 (top) or FITC Armenian hamster IgG isotype control (bottom).
Compare all formats See FITC spectral data
Cat # Size Price Save
117305 50 µg ¥11,880
117306 500 µg ¥25,520
Description

CD11c is a 150 kD glycoprotein also known as αX integrin, CR4, and p150. CD11c forms a αXβ2 heterodimer with β2 integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. The αXβ2 integrin plays an important role in cell-cell contact by binding its ligands: iC3b, fibrinogen, and CD54.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse spleen dendritic cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, immunofluorescence microscopy5, 9 (Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10), and spatial biology (IBEX)22,23.

Application References

(PubMed link indicates BioLegend citation)
  1. Granucci F, et al. 1997. J. Immunol. 159:1794.
  2. Stokes RW, et al. 1998. J. Immunol. 160:5514.
  3. Metlay JP, et al. 1990. J. Exp. Med. 171:1753. (IHC, IP)
  4. Ma XT, et al. 2006. Cancer Research 66:1169.
  5. Chin RK, et al. 2006. J. Immunol. 177:290. (IF)
  6. Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC) PubMed
  7. Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) PubMed
  8. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
  9. You Y, et al. 2009. J. Immunol. 182:7343. (IF) PubMed
  10. Roland CL, et al. 2009. Mol. Cancer Res. 8:1761. (IHC, FC) PubMed
  11. Wikstrom M, et al.2006. J. Immunol. 177:913. PubMed
  12. Pericolini E, et al. 2008. J. Leukocyte Biol. 83:1286. PubMed
  13. Randall LM, et al. 2008. Infect. Immun.76:3312. PubMed
  14. Fahlen-Yrild L, et al. 2009. J. Immunol. 183:5032. PubMed
  15. Osterholzer JJ, et al. 2009. J. Immunol. 183:8044. PubMed
  16. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  17. Eisenach PA, et al. 2010. J Cell Sci. 123:4182. PubMed
  18. Leppin K, et al. 2014. Invest. Ophthalmol. Vis. Sci. 55:3603. PubMed
  19. Sakai F, et al. 2014. PLoS One. 9:105370. PubMed
  20. Gibbins JD, et al. 2014. Blood. 124:2953. PubMed
  21. White CE, et al. 2015. J Immunol. 194:697. PubMed
  22. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  23. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  24. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  2. Man SM et al. 2016. Cell. 167(2):382-396 . PubMed
  3. Jiang W, et al. 2017. Sci Rep. 7:6501. PubMed
  4. Petrova T, et al. 2020. Sci Rep. 10:3479. PubMed
  5. Zhao J, et al. 2021. Emerg Microbes Infect. 10:913. PubMed
  6. Zhu Y, et al. 2022. Nat Commun. 13:4282. PubMed
  7. Mi Z, et al. 2021. Vaccines (Basel). 10: . PubMed
  8. Corral D, et al. 2022. Cell Rep. 39:110715. PubMed
  9. Lu R, et al. 2022. Scand J Immunol. 96:e13177. PubMed
  10. Yue Y, et al. 2022. Nat Biomed Eng. 6:898. PubMed
  11. Yu C, et al. 2022. Invest Ophthalmol Vis Sci. 63:1. PubMed
  12. Minichino D, et al. 2022. J Leukoc Biol. 112:1089. PubMed
  13. Sun Y, et al. 2023. Bioact Mater. 22:47. PubMed
  14. Zhang Z, et al. 2022. Integr Cancer Ther. 21:15347354221138534. PubMed
  15. Zhu J, et al. 2022. Nat Commun. 13:7466. PubMed
  16. Yu J, et al. 2022. Nat Commun. 13:7903. PubMed
  17. Urata S, et al. 2023. Microbiol Immunol. 67:105. PubMed
  18. Ding D, et al. 2023. J Med Chem. 66:1467. PubMed
  19. Jin SM, et al. 2023. Nat Nanotechnol. :. PubMed
  20. Liu H, et al. 2023. Cell Death Differ. :. PubMed
  21. Li X, et al. 2022. Cell. 185:1709. PubMed
  22. Bruggemann TR, et al. 2022. iScience. 25:105185. PubMed
  23. Bussières-Marmen S, et al. 2017. Cell Mol Immunol. 10.1038/cmi.2016.72. PubMed
  24. Tan J, et al. 2021. iScience. 24(8):102835. PubMed
  25. Akhtar MN, et al. 2022. MBio. 13:e0330921. PubMed
  26. Li Q, et al. 2022. Biomed Pharmacother. 153:113459. PubMed
  27. Schroeter CB, et al. 2022. J Neuroinflammation. 19:270. PubMed
  28. Chen H, et al. 2023. Nat Commun. 14:941. PubMed
  29. Wilson NG, et al. 2023. iScience. 26:105991. PubMed
  30. Lu C, et al. 2023. Antiviral Res. 212:105556. PubMed
  31. Cheng K, et al. 2023. Small. 19:e2300125. PubMed
  32. Sang H, et al. 2023. Adv Clin Exp Med. 32:551. PubMed
  33. Huang T, et al. 2023. Front Immunol. 14:1124152. PubMed
  34. Fu S, et al. 2023. Nat Commun. 14:2248. PubMed
  35. Zhou X, et al. 2023. Sci Rep. 13:6701. PubMed
  36. Li T, et al. 2023. Nat Commun. 14:2498. PubMed
  37. Wu N, et al. 2023. Front Bioeng Biotechnol. 11:1173381. PubMed
  38. Wang K, et al. 2023. Nat Commun. 14:2950. PubMed
  39. Feola S, et al. 2022. Front Immunol. 13:826164. PubMed
  40. Wilhelm J, et al. 2021. J Control Release. 329:353. PubMed
  41. Larson-Casey JL, et al. 2021. FASEB J. 35:e21675. PubMed
  42. Gonalves S, et al. 2021. Cell Reports. 34(11):108860. PubMed
  43. Kim JS, et al. 2020. Immunity. 54(1):176-190.e7. PubMed
  44. Furuta Y, et al. 2017. PLoS One. 12(2):e0172509. PubMed
  45. Engel O, et al. 2015. Stroke. 46: 3232 - 3240. PubMed
  46. Chen Y, et al. 2018. EBioMedicine. 32:31. PubMed
  47. Zhu H, et al. 2018. Elife. 7:. PubMed
  48. Devenport SN, et al. 2021. JCI Insight. 6: . PubMed
  49. Jiang W, et al. 2021. Oncol Lett. 22:625. PubMed
  50. Johnson KD, et al. 2022. Blood Adv. 6:1464. PubMed
  51. Chen X, et al. 2021. Theranostics. 11:4655. PubMed
  52. Zhang S, et al. 2022. Acta Pharm Sin B. 12:3124. PubMed
  53. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  54. Pott J et al. 2018. Cell host & microbe. 23(2):191-202 . PubMed
  55. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  56. Achmus L, et al. 2020. Front Neurol. 11:577971. PubMed
  57. Ma C, et al. 2021. Signal Transduct Target Ther. 6:353. PubMed
  58. Xing J, et al. 2016. Nat Immunol. 17:1373-1380. PubMed
  59. Wang G, et al. 2021. Nat Commun. 12:5733. PubMed
  60. Schwartz AJ, et al. 2021. Nat Metab. 3:969. PubMed
  61. Mathur S, et al. 2021. JCI Insight. 6:. PubMed
  62. Hoffman D, et al. 2021. Immunity. 54:2712. PubMed
  63. Ohtsuki T, et al. 2016. J Virol. 90: 300 - 307. PubMed
  64. Wang X, et al. 2021. Front Cell Dev Biol. 632805:9. PubMed
  65. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  66. Wang C, et al. 2021. Cell Rep. 37:110021. PubMed
  67. Tanaka Y, et al. 2021. Int J Mol Sci. 22:. PubMed
  68. Wu J, et al. 2021. Mol Med Rep. 23:. PubMed
  69. Jiang S, et al. 2020. Scand J Immunol. e12867:91. PubMed
  70. Cheng K, et al. 2021. Nat Commun. 12:2041. PubMed
  71. Wang Z, et al. 2021. J Virol. 95:e0141421. PubMed
  72. Wang B, et al. 2022. Nat Commun. 13:3821. PubMed
  73. Shin JE, et al. 2021. Heliyon. 7:e08433. PubMed
  74. Mizraji* G, et al. 2013. J Vis Exp. 77: 50388. PubMed
  75. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  76. Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed
  77. Xiao Y, et al. 2022. Clin Transl Med. 12:e1071. PubMed
  78. Shen J, et al. 2022. Mater Today Bio. 16:100416. PubMed
  79. Zhang L, et al. 2021. J Immunother Cancer. 9:. PubMed
  80. Stump CT, et al. 2021. Open Biol. 11:210245. PubMed
  81. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  82. Chen L, et al. 2021. Cell Death Differ. 28:1880. PubMed
  83. Jin R, et al. 2020. Mol Cancer Ther. . PubMed
  84. Marjanovic ND, et al. 2020. Cancer Cell. 38(2):229-246.e13. PubMed
  85. Ippolito G, et al. 2014. Proc Natl Acad Sci U S A. 111:998. PubMed
  86. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  87. Shi R, et al. 2022. Theranostics. 12:875. PubMed
  88. Coe D, et al. 2022. JCI Insight. 7:. PubMed
  89. El-Zaatari M, et al. 2014. J Immunol. 193:807. PubMed
  90. Omori S, et al. 2021. Cell Reports. 34(6):108734. PubMed
  91. Suresh MV, et al. 2019. JCI Insight. 4:e133103. PubMed
  92. Wan X, et al. 2018. Nature. 560:107. PubMed
  93. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  94. Zhao X, et al. 2022. Nat Protoc. 17:2240. PubMed
  95. Harnett MM, et al. 2022. Front Immunol. 13:953053. PubMed
  96. Dobosz E, et al. 2021. Dis Model Mech. :14. PubMed
  97. Bharali D, et al. 2008. Nanomedicine. 4:311. PubMed
  98. Xue RY, et al. 2019. Front Immunol. 10:1372. PubMed
  99. Fu Z, et al. 2021. Cell Res. 31:631. PubMed
  100. Zhu Y, et al. 2021. EMBO J. 40:e105320. PubMed
  101. Mao L, et al. 2022. Front Immunol. 13:833418. PubMed
  102. Holokai L, et al. 2020. Cancers (Basel). 12:00. PubMed
  103. Morris AB, et al. 2020. Immunity. 52(1):136-150.e6.. PubMed
  104. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  105. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  106. Sydney Lavoie et al. 2019. eLife. 8 pii: e39982. PubMed
  107. Han F, et al. 2020. Invest Ophthalmol Vis Sci. 61:24. PubMed
  108. Kostadinova E, et al. 2016. Sci Rep. 6:30943. PubMed
  109. Sakai F, et al. 2014. PLoS One. 9:105370. PubMed
  110. Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
  111. Li J, et al. 2022. Nat Commun. 13:4032. PubMed
  112. Han W, et al. 2020. J Immunol. 205:1125. PubMed
  113. Feng Y, et al. 2022. J Nanobiotechnology. 20:193. PubMed
  114. Zeng Q, et al. 2022. iScience. 25:105151. PubMed
  115. Benhamron S, et al. 2012. PLoS One. 7:e35602. PubMed
  116. Patankar Y, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 309: L902 - L913. PubMed
  117. Wang X, et al. 2020. Nat Commun. 4.505555556. PubMed
  118. Nabet BY et al. 2017. Cell. 170(2):352-366 . PubMed
  119. Bi E, et al. 2018. J Clin Invest. 128:4821. PubMed
  120. Li Z, et al. 2022. Nat Commun. 13:6321. PubMed
  121. Hsu M, et al. 2022. Nat Immunol. 23:581. PubMed
  122. Miki Y, et al. 2022. JCI Insight. 7:. PubMed
  123. Yi H, et al. 2021. Front Immunol. 12:719189. PubMed
  124. Stephens WZ, et al. 2021. Cell Rep. 37:109916. PubMed
  125. Resch TK, et al. 2018. Sci Rep. 0.722916667. PubMed
  126. Chin R, et al. 2006. J Immunol. 177:290. PubMed
  127. Unsinger J, et al. 2021. J Leukoc Biol. 109:697. PubMed
  128. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  129. Zhang J, et al. 2022. Biomater Res. 26:44. PubMed
  130. Jaiswal M, et al. 2014. J Leukoc Biol. 96:337. PubMed
  131. Miura N, et al. 2017. Mol Ther. 10.1016/j.ymthe.2017.01.020. PubMed
  132. Nasser H, et al. 2020. Cell Death Discov. 0.29375. PubMed
  133. Chen C, et al. 2020. Front Cell Neurosci. 14:8. PubMed
  134. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  135. Qin S, et al. 2018. Oncol Lett. 128:1283. PubMed
  136. Gu H, et al. 2021. Elife. 10:. PubMed
  137. Yang Z, et al. 2021. Nat Commun. 12:4299. PubMed
  138. Sivapatham S, et al. 2019. Front Immunol. 1.865277778. PubMed
  139. Schaftenaar FH, et al. 2019. Sci Rep. 9:17391. PubMed
  140. Barrera-Avalos C, et al. 2021. iScience. 24:103520. PubMed
  141. Jee JJ, et al. 2022. Nat Commun. 13:18. PubMed
  142. Schaffert S, et al. 2015. J Immunol. 195: 1470-1479. PubMed
  143. Poncini C, et al. 2015. J Immunol. 195: 3311 - 3324. PubMed
  144. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  145. Jain A, et al. 2020. Nat Immunol. 0.920138889. PubMed
  146. Lavoie S, et al. 2020. Gastroenterology. 158:1359. PubMed
  147. Burton OT, et al. 2018. Clin Exp Allergy. 48:288. PubMed
  148. Chen YL, et al. 2022. Front Neurosci. 16:876582. PubMed
  149. Li Z, et al. 2022. Evid Based Complement Alternat Med. 2022:7421265. PubMed
  150. Wang X, et al. 2022. Elife. 11:. PubMed
  151. Krotova K, et al. 2019. Mol Ther Oncolytics. 15:166. PubMed
  152. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  153. Chen R, et al. 2021. Front Oncol. 11:743050. PubMed
  154. Zhang M, et al. 2022. iScience. 25:104490. PubMed
  155. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  156. Ang QY, et al. 2020. Cell. 181(6):1263-1275.e16. PubMed
  157. Tran S, et al. 2020. Immunity. 53(3):627-640.e5. PubMed
  158. Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
  159. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
  160. Fusciello M, et al. 2019. Nat Commun. 4.407638889. PubMed
  161. Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed
  162. Liu WL, et al. 2019. Nat Commun. 10:3199. PubMed
  163. Bidet K, et al. 2019. NPJ Vaccines. 4:27. PubMed
  164. Cao Y, et al. 2022. Small. 18:e2203466. PubMed
  165. Lee S, et al. 2021. Autophagy. 18:1062. PubMed
  166. Lin YC, et al. 2020. Mol Med Rep. 22:4909. PubMed
  167. Matei DE, et al. 2021. Med (N Y). 2:864. PubMed
  168. Galeano Nio JL, et al. 2020. eLife. 9:00. PubMed
RRID
AB_313774 (BioLegend Cat. No. 117305)
AB_313774 (BioLegend Cat. No. 117306)

Antigen Details

Structure
Integrin α-chain, associates with integrin β2 (CD18), 150 kD
Distribution

Dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells

Function
Cellular adhesion
Ligand/Receptor
iC3b, fibrinogen
Cell Type
Dendritic cells, Epithelial cells, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lopez-Rodriguez C, et al. 1996. J. Immunol. 156:3780.

Gene ID
16411 View all products for this Gene ID
UniProt
View information about CD11c on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD11c Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD11c N418 FC
Biotin anti-mouse CD11c N418 FC,IHC-F
FITC anti-mouse CD11c N418 FC
PE anti-mouse CD11c N418 FC
Purified anti-mouse CD11c N418 FC,CyTOF®,IP,IHC
Alexa Fluor® 488 anti-mouse CD11c N418 FC
Alexa Fluor® 647 anti-mouse CD11c N418 FC,IHC,ICC,3D IHC
PE/Cyanine5 anti-mouse CD11c N418 FC
PE/Cyanine7 anti-mouse CD11c N418 FC
Brilliant Violet 605™ anti-mouse CD11c N418 FC
Alexa Fluor® 700 anti-mouse CD11c N418 FC
Pacific Blue™ anti-mouse CD11c N418 FC
APC/Cyanine7 anti-mouse CD11c N418 FC
PerCP/Cyanine5.5 anti-mouse CD11c N418 FC
PerCP anti-mouse CD11c N418 FC
Brilliant Violet 421™ anti-mouse CD11c N418 FC,IHC
Brilliant Violet 570™ anti-mouse CD11c N418 FC
Brilliant Violet 785™ anti-mouse CD11c N418 FC
Brilliant Violet 510™ anti-mouse CD11c N418 FC
Brilliant Violet 650™ anti-mouse CD11c N418 FC
Purified anti-mouse CD11c (Maxpar® Ready) N418 FC,CyTOF®
Alexa Fluor® 594 anti-mouse CD11c N418 IHC-F,3D IHC
PE/Dazzle™ 594 anti-mouse CD11c N418 FC
Brilliant Violet 711™ anti-mouse CD11c N418 FC
APC/Fire™ 750 anti-mouse CD11c N418 FC
TotalSeq™-A0106 anti-mouse CD11c N418 PG
Brilliant Violet 750™ anti-mouse CD11c N418 FC
TotalSeq™-B0106 anti-mouse CD11c N418 PG
TotalSeq™-C0106 anti-mouse CD11c N418 PG
KIRAVIA Blue 520™ anti-mouse CD11c N418 FC
Spark Blue™ 550 anti-mouse CD11c N418 FC
Spark NIR™ 685 anti-mouse CD11c N418 FC
Spark UV™ 387 anti-mouse CD11c N418 FC
Spark Red™ 718 anti-mouse CD11c N418 FC
Spark Blue™ 515 anti-mouse CD11c N418 FC
PerCP/Fire™ 806 anti-mouse CD11c N418 FC
Spark PLUS UV395™ anti-mouse CD11c N418 FC
Spark Blue™ 574 anti-mouse CD11c (Flexi-Fluor™) N418 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account