Loading...

FITC anti-mouse/human CD45R/B220 Antibody

Pricing & Availability
Clone
RA3-6B2 (See other available formats)
Regulatory Status
RUO
Other Names
B220
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
RA3-6B2_FITC
C57BL/6 mouse splenocytes stained with RA3-6B2 FITC
  • RA3-6B2_FITC
    C57BL/6 mouse splenocytes stained with RA3-6B2 FITC
Compare all formats See FITC spectral data
Cat # Size Price Save
103205 50 µg ¥6,600
103206 500 µg ¥19,650
Description

CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Cat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.

Application References

(PubMed link indicates BioLegend citation)
  1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
  2. George A, et al. 1994. J. Immunol. 152:1014. (Activ)
  3. Asensi V, et al. 1989. Immunology 68:204. (Activ)
  4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Chang C L-T, et al. 2007. J. Immunol. 178:6984.
  9. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  10. Lang GL, et al. 2008. Blood 111:2158. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  13. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  14. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  15. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Shah D, et al. 2021. Oncoimmunology. 10:1939601. PubMed
  2. Zhao J, et al. 2021. Emerg Microbes Infect. 10:913. PubMed
  3. Hutter K, et al. 2022. Front Immunol. 13:967914. PubMed
  4. Aimaitijiang A, et al. 2022. Genes Cells. 27:25. PubMed
  5. Wang B, et al. 2022. EMBO J. 41:e108946. PubMed
  6. Corral D, et al. 2022. Cell Rep. 39:110715. PubMed
  7. Xu J, et al. 2022. Invest Ophthalmol Vis Sci. 63:15. PubMed
  8. Tatsumi N, et al. 2022. STAR Protoc. 3:101845. PubMed
  9. Meacham CE, et al. 2022. Nat Cell Biol. 24:697. PubMed
  10. Bruggemann TR, et al. 2022. iScience. 25:105185. PubMed
  11. Lunardi A, et al. 2015. Cancer Discovery. 5:550. PubMed
  12. Zaman R, et al. 2021. Immunity. :. PubMed
  13. Katsumura KR, et al. 2018. Proc Natl Acad Sci U S A. 115:E10109. PubMed
  14. Zimmerman SM, et al. 2022. Oncogene. 41:4983. PubMed
  15. Senatus L, et al. 2023. Commun Biol. 6:280. PubMed
  16. Ji Y, et al. 2023. Nat Cell Biol. 25:726. PubMed
  17. Wang Z, et al. 2023. Mediators Inflamm. 2023:9810733. PubMed
  18. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  19. Zhang R, et al. 2021. Cell Mol Immunol. 18:1222. PubMed
  20. Israelow B, et al. 2020. bioRxiv. . PubMed
  21. Furuta Y, et al. 2017. PLoS One. 12(2):e0172509. PubMed
  22. Hata E, et al. 2016. Int Immunol. 28: 283 - 292. PubMed
  23. Engel O, et al. 2015. Stroke. 46: 3232 - 3240. PubMed
  24. Kaku H, et al. 2014. J Immunol. 193:5904. PubMed
  25. Wu N, et al. 2020. Cell Rep. 30:1129. PubMed
  26. Hewitson JP, et al. 2020. J Immunol. 204:2949. PubMed
  27. Johnson KD, et al. 2022. Blood Adv. 6:1464. PubMed
  28. Chen X, et al. 2021. Theranostics. 11:4655. PubMed
  29. Ota T, et al. 2013. J Immunol. 191:3179. PubMed
  30. Xia S, et al. 2014. J Leukoc Biol. 95:733. PubMed
  31. Tan X, et al. 2016. PLoS One. 11: 0160985. PubMed
  32. Bao H, et al. 2020. Mol Med Rep. 4.675. PubMed
  33. Dong L, et al. 2021. Cancer Cell. . PubMed
  34. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  35. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  36. Kelly L, et al. 2011. J Immunol. 187:3026. PubMed
  37. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  38. Ma C, et al. 2022. Proc Natl Acad Sci U S A. 119:. PubMed
  39. Zhu X, et al. 2017. Arch Oral Biol. 10.1016/j.archoralbio.2017.03.010. PubMed
  40. Jones M, et al. 2016. PLoS One. 11: 0157271. PubMed
  41. Wang X, et al. 2016. PLoS Pathog. 12: 1005367. PubMed
  42. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  43. Ohyagi M, et al. 2021. Nat Commun. 12:7344. PubMed
  44. Xu F, et al. 2022. Cell Death Discov. 8:142. PubMed
  45. Tu X, et al. 2022. Nat Commun. 13:6977. PubMed
  46. Carbonneau C, et al. 2012. Blood. 119:717. PubMed
  47. Luff DH, et al. 2021. Front Immunol. 631271:12. PubMed
  48. Dai B, et al. 2022. Theranostics. 12:7603. PubMed
  49. Tanaka Y, et al. 2021. Int J Mol Sci. 22:. PubMed
  50. Fujikura D, et al. 2013. PLoS One. 8:e55321. PubMed
  51. Delgado‐Benito V et al. 2018. Molecular cell. 72(4):636-649 . PubMed
  52. Zanotti KJ, et al. 2019. J Immunol. 202:1573. PubMed
  53. Mariani SA, et al. 2019. Immunity. 50:1439. PubMed
  54. Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed
  55. Huang L, et al. 2017. PLoS Biol.. 10.1371/journal.pbio.2001750. PubMed
  56. Nakata T, et al. 2020. Proc Natl Acad Sci U S A. 117:28930. PubMed
  57. Yang K, et al. 2022. J Clin Invest. 132:. PubMed
  58. Jing L, et al. 2022. Front Immunol. 13:864995. PubMed
  59. Wang Z, et al. 2021. J Virol. 95:e0141421. PubMed
  60. Zhang X, et al. 2021. Front Immunol. 12:680068. PubMed
  61. Ma Y, et al. 2015. J Immunol. 195: 3769 - 3780. PubMed
  62. Wong R, et al. 2020. Immunity. 53(5):1078-1094.e7. PubMed
  63. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  64. Qi X, et al. 2019. Nat Med. 25:1225. PubMed
  65. Zhang L, et al. 2021. Methods Mol Biol. 2388:175. PubMed
  66. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  67. Nabar NR, et al. 2022. Autophagy. 18:204. PubMed
  68. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  69. Köchl R, et al. 2020. Elife. 9:00. PubMed
  70. Pang D, et al. 2015. Clin Transl Immunology. 4: e41. PubMed
  71. Park HB, et al. 2020. Oncoimmunology. 9:1772663. PubMed
  72. Wang GZ, et al. 2019. Nat Commun. 10:1125. PubMed
  73. Mrdjen D et al. 2018. Immunity. 48(2):380-395 . PubMed
  74. Marinescu CI, et al. 2021. Stem Cell Res Ther. 12:319. PubMed
  75. Zhu Y, et al. 2022. Clin Transl Med. 12:e887. PubMed
  76. Lu X, et al. 2016. Nat Commun. 7: 12719. PubMed
  77. Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed
  78. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  79. DeVito NC, et al. 2021. Cell Reports. 35(5):109071. PubMed
  80. Noah AC, et al. 2020. J Appl Physiol (1985). 473:128. PubMed
  81. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  82. Hutter K, et al. 2020. FEBS J. . PubMed
  83. Greenwald AC, et al. 2019. J Exp Med. 216:215. PubMed
  84. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  85. Paul S, et al. 2018. Appl Microbiol Biotechnol. 102:8895. PubMed
  86. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  87. Elahi S, et al. 2020. Stem Cell Res. 43:101710. PubMed
  88. Zhang YN, et al. 2020. EBioMedicine. 56:102819. PubMed
  89. Siamishi I, et al. 2020. Cell Reports. 31(11):107756. PubMed
  90. St Clair JB, et al. 2017. PLoS One. 12:e0170556. PubMed
  91. Roy K, et al. 2019. Immunity. 50:616. PubMed
  92. Garo LP, et al. 2019. Cell Rep. 28:3353. PubMed
  93. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  94. Li H, et al. 2020. Nature. 584:274. PubMed
  95. Gu X, et al. 2022. Vaccines (Basel). 10:. PubMed
  96. Ku C, et al. 2015. Genes Dev. 29: 1930-1941. PubMed
  97. Lim CX, et al. 2020. Cell Rep. 3793:30. PubMed
  98. Xiang W, et al. 2018. Nat Commun. 9:2574. PubMed
  99. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  100. Wittner J, et al. 2022. Mucosal Immunol. 15:668. PubMed
  101. Kang X, et al. 2022. J Immunol Res. 2022:8118577. PubMed
  102. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  103. Hayashi K, et al. 2015. Int Immunol. 27: 435-445. PubMed
  104. Morrison V, et al. 2015. J Immunol. 195: 105 - 115. PubMed
  105. Jamali A, et al. 2020. Cell Reports. 32(9):108099. PubMed
  106. Liu J, et al. 2021. Immun Inflamm Dis. 9:299. PubMed
  107. Liu R, et al. 2021. Mol Cell Biol. 41:e0025121. PubMed
  108. Chen C, et al. 2021. J Virol. 95:e0082921. PubMed
  109. Chen RJ, et al. 2022. iScience. 25:105595. PubMed
  110. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
  111. Liu R, et al. 2018. Front Immunol. 9:592. PubMed
  112. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
  113. Morelli AE, et al. 2020. Cell Rep. 30:3448. PubMed
  114. Zhang W, et al. 2020. Nat Commun. 11:1187. PubMed
  115. Zhang C, et al. 2021. J Virol. 95:e0079021. PubMed
  116. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  117. Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
  118. Hsieh Y, et al. 2014. Infect Immun . 82:2087. PubMed
  119. Baomei Wang et al. 2019. Cell reports. 26(6):1614-1626 . PubMed
  120. Weng TY, et al. 2018. Sci Rep. 8:8216. PubMed
  121. Nguyen HTT, et al. 2021. PLoS Pathog. 17:e1010026. PubMed
  122. Soria-Castro R, et al. 2020. Sci Rep. 10:17802. PubMed
  123. Al-Barwani F, et al. 2014. PLoS One. 9:104523. PubMed
  124. Younes AI, et al. 2021. Transl Oncol. 14:100983. PubMed
  125. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  126. Schuler F, et al. 2017. Nat Commun. . 10.1038/s41467-017-01850-4. PubMed
  127. Laban H, et al. 2018. J Cell Biol. 217:1503. PubMed
  128. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  129. Raju S, et al. 2020. Cell Reports. 29(9):2556-2564.e3.. PubMed
  130. Sanchez HN, et al. 2020. Nat Commun. 0.5. PubMed
  131. Zheng Y, et al. 2022. J Immunol. 208:501. PubMed
  132. Ghezraoui H, et al. 2018. Nature. 560:122. PubMed
  133. Tian D, et al. 2019. Nat Commun. 10:4246. PubMed
  134. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  135. Tsyklauri O, et al. 2021. EMBO Rep. 22:e50785. PubMed
  136. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  137. Hoechst B, et al. 2016. J Immunol. 195:1517-1523. PubMed
  138. Wu Y, et al. 2015. J Immunol. 195: 2612-2623. PubMed
  139. Takahashi T, et al. 2017. J Exp Med. 10.1084/jem.20160247. PubMed
  140. Li K, et al. 2020. Nat Commun. 4.844444444. PubMed
  141. Golan K et al. 2018. Cell stem cell. 23(4):572-585 . PubMed
  142. Zegarra‐Ruiz DF et al. 2018. Cell host & microbe. 25(1):113-127 . PubMed
  143. Velazquez VM, et al. 2017. Mol Ther Methods Clin Dev. 0.277083333. PubMed
  144. Ota T, et al. 2011. J Exp Med. 208:617. PubMed
  145. Pan J, et al. 2022. Front Immunol. 13:812924. PubMed
  146. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  147. Iwanami N, et al. 2020. iScience. 23:101260. PubMed
  148. Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed
  149. Burton OT, et al. 2018. Clin Exp Allergy. 48:288. PubMed
  150. Nakamura K, et al. 2018. Cancer Cell. 33:634. PubMed
  151. Awida Z, et al. 2021. Int J Mol Sci. 23:. PubMed
  152. Ioanna E Galani et al. 2017. Immunity. 46(5):875-890 . PubMed
  153. Jing Y, et al. 2019. J Allergy Clin Immunol. 144:1377. PubMed
  154. Tham SM, et al. 2021. Biomedicines. 9:. PubMed
  155. Uematsu T, et al. 2015. Sci Rep. 5: 17577. PubMed
RRID
AB_312990 (BioLegend Cat. No. 103205)
AB_312990 (BioLegend Cat. No. 103206)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

B cells, T cell subset, NK cell subset

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt
View information about CD45R on UniProt.org

Other Formats

View All CD45R/B220 Reagents Request Custom Conjugation
Description Clone Applications
Alexa Fluor® 594 anti-mouse/human CD45R/B220 RA3-6B2 IHC-F,FC,3D IHC
APC anti-mouse/human CD45R/B220 RA3-6B2 FC
Biotin anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F
FITC anti-mouse/human CD45R/B220 RA3-6B2 FC
PE anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Cyanine5 anti-mouse/human CD45R/B220 RA3-6B2 FC
Purified anti-mouse/human CD45R/B220 RA3-6B2 FC,CyTOF®,IHC-F,IHC-P,IP,Activ
PE/Cyanine7 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Cyanine7 anti-mouse/human CD45R/B220 RA3-6B2 FC
Alexa Fluor® 488 anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,3D IHC
Alexa Fluor® 647 anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,3D IHC,SB
Pacific Blue™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Alexa Fluor® 700 anti-mouse/human CD45R/B220 RA3-6B2 FC
PerCP anti-mouse/human CD45R/B220 RA3-6B2 FC
PerCP/Cyanine5.5 anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 421™ anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F
Brilliant Violet 570™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 650™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 605™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 785™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 510™ anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,SB
Purified anti-mouse/human CD45R/B220 (Maxpar® Ready) RA3-6B2 FC,CyTOF®
Brilliant Violet 711™ anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Dazzle™ 594 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Fire™ 750 anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 750™ anti-mouse/human CD45R/B220 RA3-6B2 FC
TotalSeq™-A0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
Spark Blue™ 550 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark NIR™ 685 anti-mouse/human CD45R/B220 RA3-6B2 FC
TotalSeq™-B0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
Ultra-LEAF™ Purified anti-mouse/human CD45R/B220 RA3-6B2 FC,CyTOF®,IHC-F,IHC-P,IP,Activ
TotalSeq™-C0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
PE/Fire™ 640 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Fire™ 810 anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Fire™ 700 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark Violet™ 538 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark YG™ 581 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark YG™ 570 anti-mouse/human CD45R/B220 RA3-6B2 IHC-F
PE/Fire™ 810 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark Blue™ 574 anti-mouse/human CD45R/B220 Antibody RA3-6B2 FC
Spark Violet™ 423 anti-mouse/human CD45R/B220 Antibody RA3-6B2 FC
Spark Red™ 718 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark UV™ 387 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark PLUS UV395™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Go To Top Version: 2    Revision Date: 04/25/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account