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PE/Cyanine7 anti-human CD4 Antibody

Pricing & Availability
Clone
RPA-T4 (See other available formats)
Regulatory Status
RUO
Workshop
IV T114
Other Names
T4
Isotype
Mouse IgG1, κ
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Product Citations
publications
RPA-T4_PECy7_060507
Human peripheral blood lymphocytes stained with RPA-T4 PE/Cyanine7
  • RPA-T4_PECy7_060507
    Human peripheral blood lymphocytes stained with RPA-T4 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Save
300511 25 tests ¥25,460
300512 100 tests ¥51,980
Description

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules, and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The CD4 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections3,4,5, blocking of T cell activation1,2, and spatial biology (IBEX)10,11.  This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300569 - 300574).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. (Activ)
  2. Moir S, et al. 1999. J. Virol. 73:7972. (Activ)
  3. Deng MC, et al. 1995. Circulation 91:1647. (IHC)
  4. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  5. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  6. Lan RY, et al. 2006. Hepatology 43:729.
  7. Zenaro E, et al. 2009. J. Leukoc. Biol. 86:1393. (FC) PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Ratnapriya S, et al. 2021. Cell Reports. 36(9):109622. PubMed
  2. Toepfner N et al. 2018. eLife. 7 pii: e29213. PubMed
  3. Li M, et al. 2021. J Clin Invest. 131:. PubMed
  4. Zhang B, et al. 2023. Signal Transduct Target Ther. 8:28. PubMed
  5. Jaiswal A, et al. 2022. Cancer Cell. 40:524. PubMed
  6. Tate T, et al. 2023. Cancers (Basel). 15:. PubMed
  7. Rose JR, et al. 2023. Commun Biol. 6:363. PubMed
  8. Saotome K, et al. 2023. Nat Commun. 14:2401. PubMed
  9. Nunes-Santos CJ, et al. 2023. Nat Commun. 14:3708. PubMed
  10. Malinarich F, et al. 2015. J Immunol. 194:5174. PubMed
  11. Nesterenko PA, et al. 2021. Cell Rep. 37:110167. PubMed
  12. Muliaditan T, et al. 2021. Cell Rep Med. 2:100457. PubMed
  13. Roberts A, et al. 2021. Sci Rep. 11:4030. PubMed
  14. Chung B, et al. 2012. PLoS One. 7:e42011. PubMed
  15. Wu VH, et al. 2020. JCI Insight. 5:00. PubMed
  16. Frazzette N, et al. 2020. NPJ Precis Oncol. 4:13. PubMed
  17. Preglej T, et al. 2020. JCI Insight. 5(4):. PubMed
  18. Yu N, et al. 2018. Genes Immun. 10.1038/s41435-017-0007-7. PubMed
  19. Rochat MA, et al. 2018. Virol J. 15:191. PubMed
  20. Arra A, et al. 2021. Oncoimmunology. 10:1938475. PubMed
  21. Park JA, et al. 2021. J Hematol Oncol. 14:142. PubMed
  22. Romero-Olmedo AJ, et al. 2022. Nat Microbiol. 7:195. PubMed
  23. Kobayashi Y, et al. 2020. Int J Oncol. 999:56. PubMed
  24. Leite NC, et al. 2020. Cell Reports. 32(2):107894.. PubMed
  25. Kim ST, et al. 2022. Nat Commun. 13:1970. PubMed
  26. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  27. Albanese M, et al. 2022. Nat Methods. 19:81. PubMed
  28. Kim D, et al. 2020. Nat Med. 26:236. PubMed
  29. Wei C, et al. 2016. Cell Death Dis. 7:e2489. PubMed
  30. Park JS, et al. 2022. Arthritis Res Ther. 24:254. PubMed
  31. Chung B, et al. 2015. PLoS One. 10: 0145258. PubMed
  32. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  33. Audigé A, et al. 2017. BMC Immunology. 10.1186/s12865-017-0209-9. PubMed
  34. Brunsvig PF, et al. 2020. Front Immunol. 11:572172. PubMed
  35. Terzioglu G,et al. 2017. Mech Ageing Dev. . 10.1016/j.mad.2017.09.003. PubMed
  36. Masuda N, et al. 2021. BMC Cancer. 21:1269. PubMed
  37. Rousset F, et al. 2018. Mol Ther Nucleic Acids. 14:351. PubMed
  38. Min HK, et al. 2021. Immune Netw. 21:e43. PubMed
  39. Frazzette N, et al. 2020. NPJ Precis Oncol. 4:13. PubMed
  40. Dai B, et al. 2021. Cell Reports Medicine. 2(8):100381. PubMed
  41. Hassounah NB, et al. 2019. Cancer Immunol Immunother. 68:407. PubMed
  42. Carrion B, et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8:. PubMed
  43. Nesterenko PA, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
RRID
AB_314079 (BioLegend Cat. No. 300511)
AB_314079 (BioLegend Cat. No. 300512)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Dendritic cells, Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.

Other Formats

View All CD4 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD4 RPA-T4 FC
Biotin anti-human CD4 RPA-T4 FC
FITC anti-human CD4 RPA-T4 FC,SB
PE anti-human CD4 RPA-T4 FC
PE/Cyanine5 anti-human CD4 RPA-T4 FC
PE/Cyanine7 anti-human CD4 RPA-T4 FC
Purified anti-human CD4 RPA-T4 FC,CyTOF®,Activ,Block,IHC
APC/Cyanine7 anti-human CD4 RPA-T4 FC
Alexa Fluor® 488 anti-human CD4 RPA-T4 FC,ICC
Alexa Fluor® 647 anti-human CD4 RPA-T4 FC,SB
Pacific Blue™ anti-human CD4 RPA-T4 FC
Brilliant Violet 421™ anti-human CD4 RPA-T4 FC,ICC
Alexa Fluor® 700 anti-human CD4 RPA-T4 FC,SB
PerCP anti-human CD4 RPA-T4 FC
PerCP/Cyanine5.5 anti-human CD4 RPA-T4 FC
Brilliant Violet 570™ anti-human CD4 RPA-T4 FC
Brilliant Violet 650™ anti-human CD4 RPA-T4 FC
Purified anti-human CD4 (Maxpar® Ready) RPA-T4 FC,CyTOF®
Alexa Fluor® 594 anti-human CD4 RPA-T4 FC,ICC
Brilliant Violet 510™ anti-human CD4 RPA-T4 FC
PE/Dazzle™ 594 anti-human CD4 RPA-T4 FC
Brilliant Violet 785™ anti-human CD4 RPA-T4 FC
Brilliant Violet 605™ anti-human CD4 RPA-T4 FC
Brilliant Violet 711™ anti-human CD4 RPA-T4 FC
APC/Fire™ 750 anti-human CD4 RPA-T4 FC
CD4 Fluorophore Sampler Kit RPA-T4 FC
CD4 Fluorophore Sampler Kit with Veri-Cells™ PBMC RPA-T4 FC
TotalSeq™-A0072 anti-human CD4 RPA-T4 PG
TotalSeq™-B0072 anti-human CD4 RPA-T4 PG
TotalSeq™-C0072 anti-human CD4 RPA-T4 PG
Ultra-LEAF™ Purified anti-human CD4 RPA-T4 FC,CyTOF®,Activ,Block,IHC
TotalSeq™-D0072 anti-human CD4 RPA-T4 PG
Go To Top Version: 2    Revision Date: 07/13/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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