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PerCP/Cyanine5.5 anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
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Product Citations
publications
HK1dot4_PerCPCy55_061909.jpg
C57BL/6 bone marrow cells stained with HK1.4 PerCP/Cyanine5.5
  • HK1dot4_PerCPCy55_061909.jpg
    C57BL/6 bone marrow cells stained with HK1.4 PerCP/Cyanine5.5
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Save
128011 25 µg ¥31,020
128012 100 µg ¥73,700
Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References

(PubMed link indicates BioLegend citation)
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. Marchelletta RR, et al. 2021. J Clin Invest. 131:. PubMed
  2. Xiang G, et al. 2022. Cell Death Dis. 13:451. PubMed
  3. Schädlich IS, et al. 2022. iScience. 25:104470. PubMed
  4. Hutter K, et al. 2022. Front Immunol. 13:967914. PubMed
  5. Cohen K, et al. 2021. Cell Rep. 37:110026. PubMed
  6. Kang H, et al. 2022. Adv Mater. 34:e2106500. PubMed
  7. Dolfi B, et al. 2022. Cell Rep. 39:110949. PubMed
  8. Simpson AP, et al. 2022. Cell Rep. 40:111099. PubMed
  9. Kang J, et al. 2023. JCI Insight. 8: . PubMed
  10. Schick J, et al. 2023. Elife. 12: . PubMed
  11. Tunali G, et al. 2023. J Clin Invest. :. PubMed
  12. Ballesteros I, et al. 2014. J Vis Exp. 94: 52031. PubMed
  13. Gallizioli M, et al. 2020. Cell Rep. 33:108291. PubMed
  14. Lin C et al. 2019. Immunity. 50(1):137-151 . PubMed
  15. Turecamo SE, et al. 2018. Matrix Biol. 67:01:00. PubMed
  16. Yu X, et al. 2020. Nat Commun. 11:1110. PubMed
  17. Awida Z, et al. 2022. Int J Mol Sci. 23:. PubMed
  18. Asare-Bediako B, et al. 2022. Cells. 11:. PubMed
  19. Olofsen PA, et al. 2022. Cells. 11:. PubMed
  20. Banerjee K, et al. 2023. Eur J Immunol. 53:e2250161. PubMed
  21. van Elsas MJ, et al. 2023. J Immunother Cancer. 11:. PubMed
  22. Garabuczi É, et al. 2023. Front Immunol. 14:1139204. PubMed
  23. Latour YL, et al. 2023. Gut Microbes. 15:2192623. PubMed
  24. Park JA, et al. 2023. J Immunother Cancer. 11:. PubMed
  25. Solier S, et al. 2023. Nature. 617:386. PubMed
  26. Seclì L, et al. 2023. J Immunother Cancer. 11:. PubMed
  27. Jong RM, et al. 2022. J Immunol. 208:407. PubMed
  28. Tang L, et al. 2021. Oncol Lett. 22:605. PubMed
  29. Israelow B, et al. 2020. bioRxiv. . PubMed
  30. Gonalves S, et al. 2021. Cell Reports. 34(11):108860. PubMed
  31. Fachi JL et al. 2019. Cell reports. 27(3):750-761 . PubMed
  32. Song J, et al. 2021. J Am Heart Assoc. 10:e017329. PubMed
  33. Xu ZH, et al. 2021. Mediators Inflamm. 2021:8856326. PubMed
  34. Mylonas K, et al. 2015. Immunobiology. 220: 924-933. PubMed
  35. Leach SM, et al. 2020. Cell Rep. 33:108337. PubMed
  36. Yilmaz B, et al. 2021. Cell Host Microbe. 29(4):650-663.e9. PubMed
  37. Zhang K, et al. 2018. PLoS Pathog. 14:e1006925. PubMed
  38. Huard A, et al. 2021. J Immunol. 206:1058. PubMed
  39. Demars A, et al. 2021. PLoS Pathog. 17:e1009887. PubMed
  40. Yang Y, et al. 2020. Cell Death Discov. 0.293055556. PubMed
  41. Park JA, et al. 2021. J Hematol Oncol. 14:142. PubMed
  42. Rao S, et al. 2017. Cell. 168(3):503-516.e12. PubMed
  43. Shemer A, et al. 2020. Immunity. 53(5):1033-1049.e7. PubMed
  44. Di Gioia M, et al. 2020. Nat Immunol. 21:42. PubMed
  45. Han YH, et al. 2021. Science. 373:. PubMed
  46. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  47. Zhou Z, et al. 2019. Oxid Med Cell Longev. 2019:1209307. PubMed
  48. Zhang Y, et al. 2015. PLoS Pathog. 11: e1005167. PubMed
  49. Stutchfield B, et al. 2015. Gastroenterology. 149: 1896-1909.e14. PubMed
  50. Jeljeli M, et al. 2020. Cell Rep. 33:108325. PubMed
  51. McCreedy DA et al. 2018. eNeuro. 5(4) pii: ENEURO. PubMed
  52. Forbester JL, et al. 2020. J Virol. 94:. PubMed
  53. Cassidy BR, et al. 2020. J Neuroinflammation. 17:259. PubMed
  54. Honarpisheh P, et al. 2020. J Neuroinflammation. 0.9625. PubMed
  55. Tanaka T, et al. 2015. J Immunol. 194:2004. PubMed
  56. Lagares D, et al. 2017. Sci Transl Med. 9:eaal3765. PubMed
  57. Varol D et al. 2017. Immunity. 46(6):1030-1044 . PubMed
  58. Horn KJ, et al. 2022. Nat Commun. 13:3321. PubMed
  59. Okubo K, et al. 2021. Cell Reports. 35(7):109142. PubMed
  60. Doonan J, et al. 2019. Nat Commun. 10:1554. PubMed
  61. Harnett MM, et al. 2022. Front Immunol. 13:953053. PubMed
  62. Golden TN, et al. 2022. Front Pharmacol. 12:761496. PubMed
  63. Panea C, et al. 2021. Commun Biol. 4:913. PubMed
  64. Brigas HC, et al. 2021. Cell Reports. 36(9):109574. PubMed
  65. Paiva-Oliveira E, et al. 2017. Histochem Cell Biol. 10.1007/s00418-017-1556-6. PubMed
  66. Qi S et al. 2016. eLife. 5 pii: e14756. PubMed
  67. Cosentino K, et al. 2022. Mol Cell. 82:933. PubMed
  68. Engler AE, et al. 2020. Cell Reports. 33(13):108553. PubMed
  69. Zeng W, et al. 2021. STAR Protocols. 2(1):100361. PubMed
  70. Medler TR et al. 2018. Cancer cell. 34(4):561-578 . PubMed
  71. Tang C, et al. 2021. Theranostics. 11:9791. PubMed
  72. Rashid MH, et al. 2021. Oncol Rep. 45:1171. PubMed
  73. Blriot C, et al. 2021. Immunity. :. PubMed
  74. Ermert D, et al. 2016. PLoS Pathog. 11: 1005043. PubMed
  75. Jamali A, et al. 2020. Cell Reports. 32(9):108099. PubMed
  76. Williams JW, et al. 2017. Circ Res. 121:662. PubMed
  77. Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed
  78. Yang Y, et al. 2020. Cell Death Discov. 6:62. PubMed
  79. Wuggenig P, et al. 2020. Commun Biol. 3:130. PubMed
  80. Ali S, et al. 2021. PLoS One. 16:e0246646. PubMed
  81. Nuro-Gyina PK, et al. 2021. J Immunol. 207:868. PubMed
  82. Ioannidis L, et al. 2016. J Immunol. 196: 1227 - 1238. PubMed
  83. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  84. Hadas R, et al. 2020. JCI Insight. 5:00. PubMed
  85. Teo T, et al. 2015. J Virol. 89: 7955-7969. PubMed
  86. Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed
  87. Pinho–Ribeiro FA, et al. 2018. Cell. 173:1083. PubMed
  88. Budai Z, et al. 2021. Cells. 10:. PubMed
  89. Fontana MF, et al. 2015. Parasite Immunol. 37: 470-478. PubMed
  90. Jiang L, et al. 2020. Cell. 183(5):1219-1233.e18. PubMed
  91. Kumari P, et al. 2021. Cell Reports. 35(3):109012. PubMed
  92. Klein O, et al. 2019. J Allergy Clin Immunol. 144:1074. PubMed
  93. Canedo T, et al. 2021. Neuropsychopharmacology. 46:2358. PubMed
  94. Nishio H, et al. 2020. Regen Ther. 14:262. PubMed
  95. Rustenhoven J, et al. 2021. Cell. 184(4):1000-1016.e27. PubMed
  96. Huo M, et al. 2017. FASEB J. 10.1096/fj.201601030R. PubMed
  97. Namkoong H, et al. 2018. PLoS Pathog. 14:e1006955. PubMed
  98. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  99. Foster G, et al. 2015. J Immunol. 195: 5380 - 5392. PubMed
  100. Yajjala V, et al. 2016. J Immunol. 196: 4196 - 4203. PubMed
  101. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  102. Hendrikx S et al. 2019. Cell reports. 26(5):1227-1241 . PubMed
  103. Walsh CM, et al. 2019. Elife. 8:e48448. PubMed
  104. Lei Y, et al. 2021. Sci Adv. 7:. PubMed
  105. Chen X, et al. 2021. Cell Death Dis. 12:1123. PubMed
  106. Freed-Pastor WA, et al. 2021. Cancer Cell. 39:1342. PubMed
  107. Heuslein J, et al. 2016. Sci Rep. 6: 27029. PubMed
  108. Jiang Y, et al. 2015. MBio. 6: e01426-15. PubMed
  109. Al-Zaeed N, et al. 2021. Cell Death Dis. 12:611. PubMed
  110. Gangoso E, et al. 2021. Cell. 184:2454. PubMed
  111. Ikezu S, et al. 2021. Mol Psychiatry. 26:1808. PubMed
  112. Awida Z, et al. 2021. Int J Mol Sci. 23:. PubMed
  113. Tran S, et al. 2020. Immunity. 53(3):627-640.e5. PubMed
  114. Valdearcos M et al. 2017. Cell metabolism. 26(1):185-197 . PubMed
  115. Orr MT, et al. 2019. NPJ Vaccines. 4:1. PubMed
  116. Sadtler K, et al. 2017. Tissue Eng Part A. 23:1044. PubMed
  117. Ioanna E Galani et al. 2017. Immunity. 46(5):875-890 . PubMed
  118. Boguslawski KM, et al. 2020. Sci Adv. 6:eaax7515. PubMed
  119. Joshi JC, et al. 2020. Cell Rep. 30:4096. PubMed
  120. Avgustinova A, et al. 2021. Cell Stem Cell. . PubMed
  121. Steinmetz O, et al. 2016. J Infect Dis. 10.1093/infdis/jiv763. PubMed
  122. Qi S, et al. 2020. Theranostics. 10:1814. PubMed
  123. Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed
  124. Yáñez A et al. 2017. Immunity. 47(5):890-902 . PubMed
RRID
AB_1659242 (BioLegend Cat. No. 128011)
AB_1659242 (BioLegend Cat. No. 128012)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 12/09/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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