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APC/Cyanine7 anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
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Product Citations
publications
HK1-4_APCCyanine7_Ly-6C_Antibody_020119
C57BL/6 mouse bone marrow cells were stained with CD8a FITC and Ly-6C (clone HK1.4) APC/Cyanine7 (left) or Rat IgG2a, κ APC/Cyanine7 isotype control (right).
  • HK1-4_APCCyanine7_Ly-6C_Antibody_020119
    C57BL/6 mouse bone marrow cells were stained with CD8a FITC and Ly-6C (clone HK1.4) APC/Cyanine7 (left) or Rat IgG2a, κ APC/Cyanine7 isotype control (right).
Compare all formats See APC/Cyanine7 spectral data
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128025 25 µg 104€
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128026 100 µg 249€
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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. Parsons M, et al. 2014. J Immunol. 192:1361. PubMed
  2. Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed
  3. Martin-Martin I, et al. 2022. Cell Rep. 39:110648. PubMed
  4. Tessaro FHG, et al. 2022. Cell Rep. 39:110977. PubMed
  5. Zhang X, et al. 2022. EBioMedicine. 84:104275. PubMed
  6. Hao J, et al. 2022. Cell Rep. 41:111804. PubMed
  7. Ullah I, et al. 2023. Cell Rep Med. 4:100893. PubMed
  8. Domingos-Pereira S, et al. 2022. Int J Mol Sci. 24: . PubMed
  9. Sieow JL, et al. 2023. Int J Mol Sci. 24: . PubMed
  10. Yang L, et al. 2023. Acta Pharmacol Sin. :. PubMed
  11. Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed
  12. Zhang Q, et al. 2022. Nat Metab. 4:1055. PubMed
  13. Olofsen PA, et al. 2022. Cells. 11:. PubMed
  14. Zhao Q, et al. 2023. Signal Transduct Target Ther. 8:40. PubMed
  15. Tang C, et al. 2023. Nat Commun. 14:1493. PubMed
  16. Dijkman K, et al. 2023. NPJ Vaccines. 8:66. PubMed
  17. Ippagunta S, et al. 2016. Proc Natl Acad Sci U S A. 113: E6162 - E6171. PubMed
  18. He J, et al. 2022. Front Immunol. 12:760138. PubMed
  19. Abdala-Valencia H, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:482. PubMed
  20. Kim AS, et al. 2021. Cell. 184(17):4414-4429.e19. PubMed
  21. Heng Y, et al. 2020. Glia. . PubMed
  22. Chou YJ, et al. 2020. Sci Rep. 10:8422. PubMed
  23. Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed
  24. Súarez-López L, et al. 2021. iScience. 24:103406. PubMed
  25. Sun CP, et al. 2021. PLoS Pathog. 17:e1009758. PubMed
  26. Devalaraja S, et al. 2020. Cell. 1098:180. PubMed
  27. de Mingo Pulido , et al. 2021. Immunity. 54(6):1154-1167.e7. PubMed
  28. Álvaro de Mingo Pulido et al. 2018. Cancer cell. 33(1):60-74 . PubMed
  29. Lin T, et al. 2015. PLoS One. 10: e0138335. PubMed
  30. Wada N, et al. 2020. Mol Metab. 37:100988. PubMed
  31. Motoyama S, et al. 2020. Cells. :9. PubMed
  32. Chan YH, et al. 2019. EMBO Mol Med. 11:e10092. PubMed
  33. Tanner MA, et al. 2021. Am J Physiol Heart Circ Physiol. 321:H633. PubMed
  34. McDowell SAC, et al. 2021. Nat Cancer. 2:545. PubMed
  35. Zhou HF, et al. 2022. Front Pharmacol. 13:778755. PubMed
  36. Royzman D, et al. 2022. Front Immunol. 13:1012647. PubMed
  37. Movita D, et al. 2015. J Virol. 89:4809. PubMed
  38. Walker FC, et al. 2021. PLoS Pathog. 17:e1009402. PubMed
  39. Fujita K, et al. 2019. Proc Natl Acad Sci U S A. 116:14714. PubMed
  40. Devalaraja S, et al. 2020. STAR Protoc. 1:100188. PubMed
  41. Hou X, et al. 2022. Cell Death Dis. 13:100. PubMed
  42. Yu S, et al. 2016. Mol Ther. 10.1038/mt.2016.175. PubMed
  43. Yin S, et al. 2016. Nat Commun. 7: 11311. PubMed
  44. Domingos-Pereira S, et al. 2019. Cancer Immunol Res. 7:621. PubMed
  45. Neupane AS, et al. 2020. Cell. 183(1):110-125.e11. PubMed
  46. Abdul Pari AA, et al. 2020. Cancer Res. 80:2586. PubMed
  47. Lim HY et al. 2018. Immunity. 49(2):326-341 . PubMed
  48. Guérin MV, et al. 2019. Nat Commun. 10:4131. PubMed
  49. Wang Y, et al. 2021. Cell Rep. 35:108948. PubMed
  50. Formaglio P, et al. 2021. Immunity. 54:2724. PubMed
  51. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  52. McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
  53. Bambouskova M, et al. 2021. Cell Reports. 34(10):108756. PubMed
  54. AM C, et al. 2015. Proc Natl Acad Sci U S A. 112 7557 . PubMed
  55. Dmitrieva‐Posocco O et al. 2019. Immunity. 50(1):166-180 . PubMed
  56. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  57. Kuhn JA, et al. 2021. Elife. 10:. PubMed
  58. Liu X, et al. 2020. Cell Host Microbe. 28(5):683-698.e6. PubMed
  59. Grauel AL, et al. 2020. Nat Commun. 4.84375. PubMed
  60. Lee L, et al. 2016. PLoS One. 11:e0167693. PubMed
  61. Maisel D, et al. 2016. PLoS One. 11: 0159716. PubMed
  62. Simões FC, et al. 2020. Nat Commun. 0.875. PubMed
  63. Luo J et al. 2018. Immunity. 49(1):107-119 . PubMed
  64. Lin J, et al. 2021. Nat Commun. 12:5321. PubMed
  65. Terauchi R, et al. 2021. PLoS One. 16:e0239108. PubMed
  66. Wang SK, et al. 2021. JCI Insight. 6: . PubMed
  67. Shepardson K, et al. 2016. MBio. 7: e00506-16. PubMed
  68. Alam Z, et al. 2020. Cell Rep. 107825:31. PubMed
  69. Mossadegh–Keller N, et al. 2019. Bio Protoc. 9:5. PubMed
  70. Mohamed E, et al. 2020. Immunity. 52(4):668-682.e7.. PubMed
  71. Swanson P, et al. 2016. PLoS Pathog. 12:e1006022. PubMed
  72. Capuccini B, et al. 2016. Sci Rep. 6:39258. PubMed
  73. de Laval B, et al. 2020. Cell Stem Cell. 26(5):657-674. PubMed
  74. Olson B, et al. 2021. Brain Behav Immun. 97:102. PubMed
  75. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  76. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  77. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  78. Walker MT, et al. 2021. Front. Allergy. 2:. PubMed
  79. Mulens-Arias V, et al. 2022. Pharmaceutics. 14:. PubMed
  80. Buerfent BC, et al. 2019. GMS Infect Dis. 7:Doc04. PubMed
  81. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  82. Nicolas-Boluda A, et al. 2021. eLife. 10:00. PubMed
  83. Fedele C, et al. 2021. J Exp Med. 218: . PubMed
  84. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  85. van de Garde M, et al. 2016. PLoS One. 11:e0166094. PubMed
  86. Baker GJ, et al. 2020. Cell Syst. 0.647222222. PubMed
  87. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  88. Gardner A, et al. 2022. J Immunother Cancer. 10:. PubMed
  89. Zhang X, et al. 2020. Glia. . PubMed
  90. Lynn GM, et al. 2020. Nat Biotechnol. 38:320. PubMed
RRID
AB_10643867 (BioLegend Cat. No. 128025)
AB_10643867 (BioLegend Cat. No. 128026)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 02/01/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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