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APC/Fire™ 750 anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
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Product Citations
publications
HK1point4_APCFire750_Ly6C_Antibody_060916
C57BL/6 mouse bone marrow cells were stained with Ly-6C (clone HK1.4) APC/Fire™ 750 (filled histogram). Open histogram represents non-stained cells. Data shown was gated on the myeloid population.
  • HK1point4_APCFire750_Ly6C_Antibody_060916
    C57BL/6 mouse bone marrow cells were stained with Ly-6C (clone HK1.4) APC/Fire™ 750 (filled histogram). Open histogram represents non-stained cells. Data shown was gated on the myeloid population.
Compare all formats See APC/Fire™ 750 spectral data
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128045 25 µg 128€
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128046 100 µg 278€
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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.

Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. Zhou R, et al. 2022. EBioMedicine. 75:103762. PubMed
  2. Al-Saafeen BH, et al. 2023. Front Immunol. 13:1017780. PubMed
  3. Gupta T, et al. 2023. Front Immunol. 14:1044703. PubMed
  4. Gabriely G, et al. 2021. iScience. 24:103347. PubMed
  5. Sadek J, et al. 2021. EMBO Mol Med. 13:e13591. PubMed
  6. Kobayashi Y, et al. 2018. PLoS One. 13:e0195008. PubMed
  7. Almutairi F, et al. 2021. Front Immunol. 12:772288. PubMed
  8. Viar K, et al. 2020. PLOS ONE. 15(6):e0235110. PubMed
  9. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  10. Rustenhoven J, et al. 2021. Cell. 184(4):1000-1016.e27. PubMed
  11. Moufarrij S, et al. 2020. Sci Rep. 10:3470. PubMed
  12. Scheyltjens I, et al. 2022. Nat Protoc. 17:2354. PubMed
  13. Chirivi RGS, et al. 2020. Cell Mol Immunol. . PubMed
RRID
AB_2616730 (BioLegend Cat. No. 128045)
AB_2616730 (BioLegend Cat. No. 128046)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org
Go To Top Version: 1    Revision Date: 06/09/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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