APC/Fire™ 750 anti-human IgM Antibody IgM, MHM-88

Pricing & Availability

Clone: MHM-88 (See other available formats)
Regulatory Status: RUO
Other Names: Immunoglobulin M
Isotype: Mouse IgG1, κ
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Product Citations: publications

MHM-88_APCFire750_IgM_Antibody_040819 — Human peripheral blood lymphocytes were cultured overnight and then stained with CD19 PE and anti-human IgM (clone MHM-88) APC/Fire™ 750 (left) or mouse IgG1, κ APC/Fire™ 750 isotype control (right).

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314545

25 tests

141€

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314546

100 tests

306€

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Description

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on immature and mature B cells, while IgM heavy (μ) chain is expressed intracellularly in pre-B cells.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.
Excitation Laser: Red Laser (633 nm)
Application Notes: MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.

Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.
RRID: AB_2800834 (BioLegend Cat. No. 314545)
AB_2800834 (BioLegend Cat. No. 314546)

Antigen Details

Structure: Ig family
Distribution: B cells
Function: B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type: B cells
Biology Area: Immunology
Gene ID: 3507 View all products for this Gene ID
UniProt: View information about IgM on UniProt.org

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Other Formats

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Purified anti-human IgM (Maxpar® Ready)	MHM-88	FC,CyTOF®
PE/Dazzle™ 594 anti-human IgM	MHM-88	FC
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Alexa Fluor® 488 anti-human IgM	MHM-88	FC
Alexa Fluor® 647 anti-human IgM	MHM-88	FC
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Brilliant Violet 711™ anti-human IgM	MHM-88	FC
TotalSeq™-A0136 anti-human IgM	MHM-88	PG
TotalSeq™-C0136 anti-human IgM	MHM-88	PG
Brilliant Violet 785™ anti-human IgM	MHM-88	FC
APC/Fire™ 750 anti-human IgM	MHM-88	FC
TotalSeq™-B0136 anti-human IgM	MHM-88	PG
Spark Violet™ 423 anti-human IgM Antibody	MHM-88	FC
TotalSeq™-D0136 anti-human IgM	MHM-88	PG
Spark Blue™ 550 anti-human IgM	MHM-88	FC
Spark Blue™ 515 anti-human IgM	MHM-88	FC
PE/Fire™ 700 anti-human IgM	MHM-88	FC
Spark YG™ 593 anti-human IgM	MHM-88	FC
PE/Fire™ 640 anti-human IgM	MHM-88	FC
Spark Red™ 718 anti-human IgM (Flexi-Fluor™)	MHM-88	FC
APC anti-human IgM	MHM-88	FC
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Brilliant Violet 750™ anti-human IgM	MHM-88	FC

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Go To Top Version: 1 Revision Date: 04/08/2019

For Research Use Only. Not for diagnostic or therapeutic use.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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