- Clone
- m24 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Integrin β2 subunit, LFA -1 β subunit, β2 integrin, b2 subunit, LFA-1b, LFA-1 beta, LFA1b, LFA1 beta, beta 2 integrin subunit LFA
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
363407 | 25 tests | 150€ | ||||
363408 | 100 tests | 317€ |
CD11/CD18 belongs to the integrin family of proteins. It is heterodimeric cell surface receptor expressed on all leukocytes. CD18, in association with integrin α chain CD11a, CD11b, and CD11c forms LFA-1, Mac-1, and αXβ2, respectively, and plays an important role in leukocyte adhesion. CD11/CD18 complexes bind ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, and fibrinogen. Clone m24 binds the extended/open high affinity conformation of CD11a/CD18. The antibody can be used as a reporter of the activation state of the integrin receptor in response to exposure to Mg2+ or Mn2+.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Fibronectin-purified human monocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
Clone m24 can be used as a reporter of the activation state of integrin receptor in response to exposure with Mg2+ or Mn2+.
- Application References
-
- Hogg N. et al. 1985. Cell Immunol. 92:247.
- McDowall A. et al. 2003. J. Clin. Invest. 111:51.
- RRID
-
AB_2716067 (BioLegend Cat. No. 363407)
AB_2716067 (BioLegend Cat. No. 363408)
Antigen Details
- Structure
- Integrin family, 95KD, Ig superfamily
- Distribution
-
All leukocytes
- Function
- Adhesion
- Ligand/Receptor
- ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, fibrinogen
- Cell Type
- Dendritic cells, Leukocytes, Neutrophils
- Biology Area
- Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Anderson D, et al. 1987. Annu. Rev. Med. 38:175.
2. Springer T. 1994. Cell 76:301. - Gene ID
- 16408 View all products for this Gene ID 16414 View all products for this Gene ID
- UniProt
- View information about CD18 on UniProt.org
Related FAQs
- What is the F/P ratio range of our BV421™ format antibody reagents?
-
It is lot-specific. On average it ranges between 2-4.
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD11a/CD18 (LFA-1)
Human peripheral blood neutrophils were stimulated with PMA ... -
Alexa Fluor® 488 anti-human CD11a/CD18 (LFA-1)
PMA-stimulated human peripheral blood granulocytes were stai... -
Brilliant Violet 421™ anti-human CD11a/CD18 (LFA-1)
PMA-stimulated human peripheral blood granulocytes were stai... -
APC anti-human CD11a/CD18 (LFA-1)
PMA-stimulated human peripheral blood granulocytes were sta... -
Alexa Fluor® 647 anti-human CD11a/CD18 (LFA-1)
PMA-stimulated human peripheral blood granulocytes were stai... -
PE anti-human CD11a/CD18 (LFA-1)
PMA-stimulated human peripheral blood granulocytes were sta... -
APC/Fire™ 750 anti-human CD11a/CD18 (LFA-1)
PMA-stimulated human peripheral blood granulocytes were sta... -
PerCP/Cyanine5.5 anti-human CD11a/CD18 LFA-1
PMA-stimulated human peripheral blood granulocytes were sta... -
PE/Cyanine7 anti-human CD11a/CD18 (LFA-1)
PMA-stimulated human peripheral blood granulocytes were sta... -
Biotin anti-human CD11a/CD18 (LFA-1)
PMA-stimulated human peripheral blood granulocytes were sta... -
FITC anti-human CD11a/CD18 (LFA-1)
PMA-stimulated human peripheral blood granulocytes were stai... -
Alexa Fluor® 700 anti-human CD11a/CD18 (LFA-1)
PMA-stimulated human peripheral blood granulocytes were stai... -
TotalSeq™-B0818 anti-human CD11a/CD18 (LFA-1)
-
TotalSeq™-C0818 anti-human CD11a/CD18 (LFA-1)
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