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FITC anti-mouse TCR β chain Antibody

Pricing & Availability
Clone
H57-597 (See other available formats)
Regulatory Status
RUO
Other Names
TCR-β chain, TCR-β, β-TCR
Isotype
Armenian Hamster IgG
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Product Citations
publications
H57-597_FITC_031808
C57BL/6 mouse splenocytes stained with H57-597 FITC
  • H57-597_FITC_031808
    C57BL/6 mouse splenocytes stained with H57-597 FITC
Compare all formats See FITC spectral data
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109205 50 µg 38€
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109206 500 µg 164€
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Description

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR-β is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex (along with TCR-α). It is expressed on α/β TCR-bearing T cells and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Affinity purified TCR from mouse DO-11.10 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

H57-597 is a hamster mAb directed to an epitope of the C region of TCR ß chain12. The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/ß TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation2, in vitro stimulation2,3, in vivo depletion4-6, and immunohistochemical staining of acetone-fixed frozen sections7,8,9. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109253-109258).

Application References
  1. Gascoigne NJ. 1990. J. Biol. Chem. 265:9296.
  2. Kruisbeek A, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Costim IP)
  3. Davenport C, et al. 1995. J. Immunol. 155:3742. (Costim)
  4. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  5. Kummer U, et al. 2001. Immunol. Lett. 75:153. (Deplete)
  6. van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
  7. Tomita K, et al. 1999. Genes Dev. 13:1203. (IHC)
  8. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  9. Ponomarev ED, et al. 2007. J. Immunol. 178:39. (IHC)
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Tsukumo S, et al. 2006. J.Immunol. 177:8365. (FC) PubMed
  12. Grégoire C, et al. 1991. Proc. Natl. Acad. Sci USA 88:8077.
Product Citations
  1. Collin R, et al. 2020. J Immunol. 205:133. PubMed
  2. Pohlmeier L, et al. 2021. Allergy. 76:2030. PubMed
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  7. Horkova V, et al. 2023. Nat Immunol. 24:174. PubMed
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  12. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
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  14. Wyatt-Johnson SK, et al. 2023. J Neuroinflammation. 20:78. PubMed
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  20. Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed
  21. Nagatake T, et al. 2022. Mucosal Immunol. 15:289. PubMed
  22. Altshuler A, et al. 2021. Cell Stem Cell. 28(7):1248-1261.e8. PubMed
  23. Gómez-Díaz C, et al. 2021. iScience. 24:103241. PubMed
  24. Shemer A, et al. 2020. Immunity. 53(5):1033-1049.e7. PubMed
  25. Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
  26. Murphy M, et al. 2017. Eur J Immunol. 47:880. PubMed
  27. Anderson CK et al. 2019. Cell Rep. 27(2):537-548 . PubMed
  28. Cong M, et al. 2021. Methods Mol Biol. 2388:59. PubMed
  29. Köchl R, et al. 2020. Elife. 9:00. PubMed
  30. Fennell LM, et al. 2020. EMBO J. 39:e103303. PubMed
  31. Ng SS, et al. 2020. Nat Immunol. 21:1205. PubMed
  32. Everard A, et al. 2014. Nat Commun. 5:5648. PubMed
  33. Satoh M, et al. 2016. Sci Rep. 6:28473. PubMed
  34. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  35. Germundson DL, et al. 2022. Front Allergy. 3:870513. PubMed
  36. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  37. Lim CX, et al. 2020. Cell Rep. 3793:30. PubMed
  38. Dodard G, et al. 2020. Cell Reports. 32(1):107855.. PubMed
  39. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
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  43. Tuong ZK, et al. 2021. iScience. 24:103326. PubMed
  44. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
  45. Miyauchi E, et al. 2020. Nature. 585:102. PubMed
  46. Liang Q, et al. 2020. Front Microbiol. 11:571847. PubMed
  47. Li F, et al. 2022. Nat Commun. 13:4334. PubMed
  48. Da Mesquita S, et al. 2021. Science Advances. 7(21):. PubMed
  49. Diercks BP, et al. 2018. Sci Signal. 11:eaat0358. PubMed
  50. Rustenhoven J, et al. 2021. Cell. 184(4):1000-1016.e27. PubMed
  51. Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed
  52. Werner A, et al. 2021. iScience. 24:103076. PubMed
  53. Fujimoto M, et al. 2022. Nat Commun. 13:5408. PubMed
  54. Hackstein CP, et al. 2022. Nat Commun. 13:7472. PubMed
  55. Wu Y, et al. 2015. J Immunol. 195: 2612-2623. PubMed
  56. Shimba A et al. 2018. Immunity. 48(2):286-298 . PubMed
  57. Wang X, et al. 2022. Elife. 11:. PubMed
  58. Nakagawa Y, et al. 2015. Immunol Lett. 167: 72-86. PubMed
  59. Chew HY, et al. 2020. Cell. 180(5):895-914. PubMed
  60. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  61. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  62. Burton OT, et al. 2018. Clin Exp Allergy. 48:288. PubMed
  63. D'Cruz L, et al. 2014. J Immunol. 192:2227. PubMed
  64. Katsuyama T, et al. 2021. Cell Reports. 36(1):109339. PubMed
  65. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
  66. Wunderlich CM, et al. 2018. Nat Commun. 9:1646. PubMed
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RRID
AB_313428 (BioLegend Cat. No. 109205)
AB_313428 (BioLegend Cat. No. 109206)

Antigen Details

Structure
Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit
Distribution

Majority of T cells and thymocytes (correlated to differentiation)

Function
Antigen recognition, T cell activation
Ligand/Receptor
Peptide bound-MHC class I and II
Antigen References

1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749.
3. Kubo R, et al. 1989. J. Immunol. 142:2736.

Gene ID
21577 View all products for this Gene ID
UniProt
View information about TCR beta chain on UniProt.org

Other Formats

View All TCR β chain Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse TCR β chain H57-597 FC
Biotin anti-mouse TCR β chain H57-597 FC,IHC-F
FITC anti-mouse TCR β chain H57-597 FC,IHC-F
PE anti-mouse TCR β chain H57-597 FC
PE/Cyanine5 anti-mouse TCR β chain H57-597 FC
Purified anti-mouse TCR β chain H57-597 FC,CyTOF®,IHC-F,IP,Costim,Depletion
Alexa Fluor® 488 anti-mouse TCR β chain H57-597 FC,IHC-F
Alexa Fluor® 647 anti-mouse TCR β chain H57-597 FC,IHC-F
APC/Cyanine7 anti-mouse TCR β chain H57-597 FC
PE/Cyanine7 anti-mouse TCR β chain H57-597 FC
Alexa Fluor® 700 anti-mouse TCR β chain H57-597 FC
Pacific Blue™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 421™ anti-mouse TCR β chain H57-597 FC,IHC-F
PerCP/Cyanine5.5 anti-mouse TCR β chain H57-597 FC
Brilliant Violet 570™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 510™ anti-mouse TCR β chain H57-597 FC,IHC-F
Purified anti-mouse TCR β chain (Maxpar® Ready) H57-597 FC,CyTOF®
Alexa Fluor® 594 anti-mouse TCR β chain H57-597 FC,IHC-F
PE/Dazzle™ 594 anti-mouse TCR β chain H57-597 FC
Brilliant Violet 605™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 711™ anti-mouse TCR β chain H57-597 FC
APC/Fire™ 750 anti-mouse TCR β chain H57-597 FC
TotalSeq™-A0120 anti-mouse TCR β chain H57-597 PG
Brilliant Violet 785™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 650™ anti-mouse TCR β chain H57-597 FC
Ultra-LEAF™ Purified anti-mouse TCR β chain H57-597 FC,CyTOF®,IHC-F,IP,Costim,Depletion
TotalSeq™-C0120 anti-mouse TCR β chain H57-597 PG
TotalSeq™-B0120 anti-mouse TCR β chain H57-597 PG
Spark Red™ 718 anti-mouse TCR β chain (Flexi-Fluor™) H57-597 FC
Spark PLUS UV395™ anti-mouse TCR β chain H57-597 FC
TotalSeq™-D0120 anti-mouse TCR β chain H57-597 PG
Spark Blue™ 574 anti-mouse TCR β chain (Flexi-Fluor™) H57-597 FC
Spark Blue™ 550 anti-mouse TCR β chain (Flexi-Fluor™) H57-597 FC
Go To Top Version: 2    Revision Date: 02/06/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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