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PerCP/Cyanine5.5 anti-mouse TCR β chain Antibody

Pricing & Availability
Clone
H57-597 (See other available formats)
Regulatory Status
RUO
Other Names
TCR-β chain, TCR-β, β-TCR
Isotype
Armenian Hamster IgG
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Product Citations
publications
H57-597_PerCPCyanine55_TCR-B-chain_Antibody_110118
C57BL/6 splenocytes were stained with CD3e APC and TCR ß chain (clone H57-597) PerCP/Cyanine5.5 (left), or Armenian Hamster IgG PerCP/Cyanine5.5 isotype control (right).
  • H57-597_PerCPCyanine55_TCR-B-chain_Antibody_110118
    C57BL/6 splenocytes were stained with CD3e APC and TCR ß chain (clone H57-597) PerCP/Cyanine5.5 (left), or Armenian Hamster IgG PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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109227 25 µg 90€
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109228 100 µg 231€
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Description

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR-β is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex (along with TCR-α). It is expressed on α/β TCR-bearing T cells and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Affinity purified TCR from mouse DO-11.10 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

H57-597 is a hamster mAb directed to an epitope of the C region of TCR ß chain12. The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/ß TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation2, in vitro stimulation2,3, in vivo depletion4-6, and immunohistochemical staining of acetone-fixed frozen sections7,8,9. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109253-109258).

Application References
  1. Gascoigne NJ. 1990. J. Biol. Chem. 265:9296.
  2. Kruisbeek A, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Costim IP)
  3. Davenport C, et al. 1995. J. Immunol. 155:3742. (Costim)
  4. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  5. Kummer U, et al. 2001. Immunol. Lett. 75:153. (Deplete)
  6. van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
  7. Tomita K, et al. 1999. Genes Dev. 13:1203. (IHC)
  8. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  9. Ponomarev ED, et al. 2007. J. Immunol. 178:39. (IHC)
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Tsukumo S, et al. 2006. J.Immunol. 177:8365. (FC) PubMed
  12. Grégoire C, et al. 1991. Proc. Natl. Acad. Sci USA 88:8077.
Product Citations
  1. Oh–Hora M, et al. 2019. J Immunol. 202:2616. PubMed
  2. Lang J, et al. 2020. Nat Commun. 11:1338. PubMed
  3. Soon MSF, et al. 2020. Nat Immunol. 1.984027778. PubMed
  4. Hutter K, et al. 2022. Front Immunol. 13:967914. PubMed
  5. Merana GR, et al. 2022. Cell Rep. 39:110891. PubMed
  6. McCauley KE, et al. 2022. Cell Rep Med. 3:100713. PubMed
  7. Theofilatos D, et al. 2022. STAR Protoc. 3:101839. PubMed
  8. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  9. Kumpunya S, et al. 2023. Front Immunol. 13:1010764. PubMed
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  11. Konjar S, et al. 2022. Proc Natl Acad Sci U S A. 119:e2202144119. PubMed
  12. Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed
  13. Lo WL, et al. 2023. Nat Immunol. 24:676. PubMed
  14. Masle-Farquhar E, et al. 2023. Front Immunol. 14:1095257. PubMed
  15. Ichikawa M, et al. 2023. Nat Commun. 14:3261. PubMed
  16. Shimizu K, et al. 2020. Molecular Cell. 77(5):937-950.e6.. PubMed
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  19. Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed
  20. Kobayashi T, et al. 2019. Cell. 176:982. PubMed
  21. Christian LS, et al. 2021. Cell Reports. 35(6):109118. PubMed
  22. Leech JM, et al. 2020. Cell Host & Microbe. 26(6):795-809.e5.. PubMed
  23. Nakamoto N, et al. 2019. Nat Microbiol. 4:492. PubMed
  24. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  25. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  26. Nakagawa S et al. 2017. Cell host & microbe. 22(5):667-677 . PubMed
  27. Elsner RA, et al. 2020. Cell Reports. 29(9):2796-2809.e5.. PubMed
  28. Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed
  29. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  30. Webster HC, et al. 2020. J Immunol Methods. 112702:477. PubMed
  31. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  32. Chen JS, et al. 2022. Sci Immunol. 7:eabl5652. PubMed
  33. Anderson CK et al. 2019. Cell Rep. 27(2):537-548 . PubMed
  34. Ostendorf BN, et al. 2020. Nat Med. 26:1048. PubMed
  35. Kunis G, et al. 2013. Brain. 136:3427. PubMed
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  39. Tong Q, et al. 2022. FEBS Open Bio. 12:1542. PubMed
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  42. Tavazoie MF, et al. 2018. Cell. 172:825. PubMed
  43. Jia Q, et al. 2021. Theranostics. 4699:11. PubMed
  44. Krollmann C, et al. 2022. STAR Protoc. 3:101309. PubMed
  45. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  46. Dodard G, et al. 2020. Cell Reports. 32(1):107855.. PubMed
  47. Gurusamy M, et al. 2021. Nat Commun. 12:6798. PubMed
  48. Covarrubias AJ, et al. 2020. Nat Metab. 1265:2. PubMed
  49. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  50. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  51. Kim M, et al. 2018. Immunity. 49:151. PubMed
  52. Da Mesquita S, et al. 2021. Science Advances. 7(21):. PubMed
  53. Shissler SC, et al. 2020. Sci Rep. 10:8218. PubMed
  54. Bhatt D, et al. 2021. J Exp Med. 218:. PubMed
  55. Asadi Shahmirzadi A, et al. 2020. Cell Metabolism. 32(3):447-456.e6. PubMed
  56. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  57. Ding L et al. 2018. Cell reports. 25(11):2972-2980 . PubMed
  58. Glodde N et al. 2017. Immunity. 47(4):789-802 . PubMed
  59. Sun T, et al. 2017. J Cell Biol. 216:199. PubMed
  60. Montes de Oca M, et al. 2016. PLoS Pathog. 12: 1005398. PubMed
  61. Nakamura K, et al. 2018. Cancer Cell. 33:634. PubMed
  62. Pelly V, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.4. PubMed
  63. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  64. Miao Y et al. 2019. Cell. 177(5):1172-1186 . PubMed
  65. Wang X, et al. 2021. Immunity. 54(6):1123-1136.e8. PubMed
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RRID
AB_1575173 (BioLegend Cat. No. 109227)
AB_1575173 (BioLegend Cat. No. 109228)

Antigen Details

Structure
Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit
Distribution

Majority of T cells and thymocytes (correlated to differentiation)

Function
Antigen recognition, T cell activation
Ligand/Receptor
Peptide bound-MHC class I and II
Antigen References

1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749.
3. Kubo R, et al. 1989. J. Immunol. 142:2736.

Gene ID
21577 View all products for this Gene ID
UniProt
View information about TCR beta chain on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All TCR β chain Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse TCR β chain H57-597 FC
Biotin anti-mouse TCR β chain H57-597 FC,IHC-F
FITC anti-mouse TCR β chain H57-597 FC,IHC-F
PE anti-mouse TCR β chain H57-597 FC
PE/Cyanine5 anti-mouse TCR β chain H57-597 FC
Purified anti-mouse TCR β chain H57-597 FC,CyTOF®,IHC-F,IP,Costim,Depletion
Alexa Fluor® 488 anti-mouse TCR β chain H57-597 FC,IHC-F
Alexa Fluor® 647 anti-mouse TCR β chain H57-597 FC,IHC-F
APC/Cyanine7 anti-mouse TCR β chain H57-597 FC
PE/Cyanine7 anti-mouse TCR β chain H57-597 FC
Alexa Fluor® 700 anti-mouse TCR β chain H57-597 FC
Pacific Blue™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 421™ anti-mouse TCR β chain H57-597 FC,IHC-F
PerCP/Cyanine5.5 anti-mouse TCR β chain H57-597 FC
Brilliant Violet 570™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 510™ anti-mouse TCR β chain H57-597 FC,IHC-F
Purified anti-mouse TCR β chain (Maxpar® Ready) H57-597 FC,CyTOF®
Alexa Fluor® 594 anti-mouse TCR β chain H57-597 FC,IHC-F
PE/Dazzle™ 594 anti-mouse TCR β chain H57-597 FC
Brilliant Violet 605™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 711™ anti-mouse TCR β chain H57-597 FC
APC/Fire™ 750 anti-mouse TCR β chain H57-597 FC
TotalSeq™-A0120 anti-mouse TCR β chain H57-597 PG
Brilliant Violet 785™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 650™ anti-mouse TCR β chain H57-597 FC
Ultra-LEAF™ Purified anti-mouse TCR β chain H57-597 FC,CyTOF®,IHC-F,IP,Costim,Depletion
TotalSeq™-C0120 anti-mouse TCR β chain H57-597 PG
TotalSeq™-B0120 anti-mouse TCR β chain H57-597 PG
Spark Red™ 718 anti-mouse TCR β chain (Flexi-Fluor™) H57-597 FC
Spark PLUS UV395™ anti-mouse TCR β chain H57-597 FC
TotalSeq™-D0120 anti-mouse TCR β chain H57-597 PG
Spark Blue™ 574 anti-mouse TCR β chain (Flexi-Fluor™) H57-597 FC
Spark Blue™ 550 anti-mouse TCR β chain (Flexi-Fluor™) H57-597 FC
Go To Top Version: 3    Revision Date: 11/01/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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