- Clone
- HI111 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- IV N231
- Other Names
- LFA-1α chain, Integrin αL subunit, αL Integrin, ITGAL
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
CD11a is a 170-180 kD type I transmembrane glycoprotein also known as LFA-1α chain and integrin αL subunit. CD11a non-covalently associates with integrin β2 (CD18) to form LFA-1. It is expressed on all leukocytes, including B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. It is absent on non-hematopoietic tissues and platelets. CD11a plays a central role in leukocyte cell-cell interactions and is important in lymphocyte costimulation. CD11a/CD18 binds to ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50).
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cow, Capuchin monkey, Chimpanzee, Cynomolgus, Dog, Horse, Rabbit, Sheep
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC
- Recommended Usage
-
Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Clone HI111 epitope maps to the top region of the I domain that is close to the putative ligand-binding site surrounding the MIDAS (metal ion-dependent adhesion site). HI111 is specific for the closed confirmation of the integrin8. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, Western blotting2, and blocking of cell-cell interaction and inhibition the binding of ICAM-14. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301233 & 301234).
- Additional Product Notes
-
For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
- Application References
-
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press New York.
- Leite F, et al. 2002. Infec. Immun. 70:4336.
- Jiang Y, et al. 2005. Clin. Hemorheol. Microcircul. 32:261.
- BTchard D, et al. 2001. J. Immunol. 167:3099.
- Sithu SD, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M611273200.
- Choi EY, et al. 2008. Blood 111:3607. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Ma Q, et al. 2002. J. Biol. Chem. 277:10638.
- RRID
-
AB_3106333 (BioLegend Cat. No. 285076)
Antigen Details
- Structure
- Integrin, type I transmembrane glycoprotein, noncovalently linked with integrin β2 (CD18) forms LFA-1, 170-180 kD
- Distribution
-
Leukocytes
- Function
- Adhesion, costimulation
- Ligand/Receptor
- ICAM-1(CD54), ICAM-2(CD102), ICAM-3(CD50)
- Cell Type
- Leukocytes, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Lub M, et al. 1995. Immunol. Today 16:479.
2. Parsons J. 1996. Curr. Opin. Cell Biol. 8:146. - Gene ID
- 3683 View all products for this Gene ID
- UniProt
- View information about CD11a on UniProt.org
Related FAQs
- What are Flexi-Fluors?
-
Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.
- How quickly will I receive my order?
-
We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.
- How are Flexi-Fluors different from regular catalog products?
-
Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.
- How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?
-
Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
- I can’t find the antibody-dye combination that I need. When will it be available?
-
We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.
- I need help to validate the performance of my Flexi-Fluor. Who should I contact?
-
Please get in touch with Technical Service for assistance.
- Can I order more than 50 μg of a Flexi-Fluor?
-
Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.
- What is the expiration date of my Flexi-Fluor?
-
Expiration dates can be found on the vial label or by using our CoA lookup tool.
Other Formats
View All CD11a Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
APC anti-human CD11a | HI111 | FC |
Biotin anti-human CD11a | HI111 | FC |
FITC anti-human CD11a | HI111 | FC |
PE anti-human CD11a | HI111 | FC |
PE/Cyanine5 anti-human CD11a | HI111 | FC |
Purified anti-human CD11a | HI111 | FC,IHC-F,WB,Block |
Alexa Fluor® 488 anti-human CD11a | HI111 | FC |
Alexa Fluor® 647 anti-human CD11a | HI111 | FC |
PE/Cyanine7 anti-human CD11a | HI111 | FC |
Alexa Fluor® 594 anti-human CD11a | HI111 | ICC,FC |
Purified anti-human CD11a (Maxpar® Ready) | HI111 | FC,CyTOF® |
APC/Fire™ 750 anti-human CD11a | HI111 | FC |
Alexa Fluor® 700 anti-human CD11a | HI111 | FC |
PerCP/Cyanine5.5 anti-human CD11a | HI111 | FC |
PE/Dazzle™ 594 anti-human CD11a | HI111 | FC |
Ultra-LEAF™ Purified anti-human CD11a | HI111 | FC,IHC-F,WB,Block |
APC/Cyanine7 anti-human CD11a | HI111 | FC |
Brilliant Violet 711™ anti-human CD11a | HI111 | FC |
Brilliant Violet 650™ anti-human CD11a | HI111 | FC |
Spark Red™ 718 anti-human CD11a (Flexi-Fluor™) | HI111 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
APC anti-human CD11a
-
Biotin anti-human CD11a
-
FITC anti-human CD11a
-
PE anti-human CD11a
-
PE/Cyanine5 anti-human CD11a
-
Purified anti-human CD11a
-
Alexa Fluor® 488 anti-human CD11a
-
Alexa Fluor® 647 anti-human CD11a
-
PE/Cyanine7 anti-human CD11a
-
Alexa Fluor® 594 anti-human CD11a
-
Purified anti-human CD11a (Maxpar® Ready)
-
APC/Fire™ 750 anti-human CD11a
-
Alexa Fluor® 700 anti-human CD11a
-
PerCP/Cyanine5.5 anti-human CD11a
-
PE/Dazzle™ 594 anti-human CD11a
-
Ultra-LEAF™ Purified anti-human CD11a
-
APC/Cyanine7 anti-human CD11a
-
Brilliant Violet 711™ anti-human CD11a
-
Brilliant Violet 650™ anti-human CD11a
-
Spark Red™ 718 anti-human CD11a (Flexi-Fluor™)
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