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PE anti-mouse CD206 (MMR) Antibody

Pricing & Availability
Clone
C068C2 (See other available formats)
Regulatory Status
RUO
Other Names
MMR (macrophage mannose receptor), MR (mannose receptor), MRC1
Isotype
Rat IgG2a, κ
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Product Citations
publications
C068C2_PE_1_082911
Thioglycollate-elicited BALB/c peritoneal macrophages were surface stained with CD107b (Mac-3) APC, and then intracellularly stained with CD206 (clone C068C2) PE (top) or rat IgG2a, κ PE isotype control (bottom).
  • C068C2_PE_1_082911
    Thioglycollate-elicited BALB/c peritoneal macrophages were surface stained with CD107b (Mac-3) APC, and then intracellularly stained with CD206 (clone C068C2) PE (top) or rat IgG2a, κ PE isotype control (bottom).
  • C068C2_PE_2_082911
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141705 25 µg 104€
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141706 100 µg 240€
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Description

CD206, also known as mannose receptor (MR), is a 175 kD type I membrane protein. It is a pattern recognition receptor (PRR) belonging to the C-type lectin superfamily. MR is expressed on macrophages, dendritic cells, Langerhans cells, and hepatic or lymphatic endothelial cells. MR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine through its C-type lectin-like carbohydrate recognition domains, sulfated carbohydrate antigens through its cysteine-rich domain, and collagens through its fibronectin type II domain. MR mediates endocytosis and phagocytosis as well as activation of macrophages and antigen presentation. It plays an important role in host defense and provides a link between innate and adaptive immunity. Recently, MR on lymphatic endothelial cells was found to be involved in leukocyte trafficking and a contributor to the metastatic behavior of cancer cells. It suggests that MR may be a potential target in controlling inflammation and cancer metastasis by targeting the lymphatic vasculature.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD206 (MMR)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
FC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone C068C2 recognizes a region similar to clone MR5D3, based on the ability of the clones to block each other. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.

Application References
  1. Keller J, et al. 2012. Biochem Biophys Res Commun. 417:217. PubMed
  2. Ito H, et al. 2012. J Am Soc Nephrol. 23:1797. PubMed
  3. Yang X, et al. 2015. PNAS. 112:2900. PubMed
  4. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  5. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed
  2. Feng Y, et al. 2018. J Am Soc Nephrol. 29:182. PubMed
  3. Seo JB, et al. 2019. Nat Metab. 1:86. PubMed
  4. Ying W, et al. 2019. Cell Metab. 29:457. PubMed
  5. Sun H, et al. 2019. Mol Hum Reprod. 25:05:00. PubMed
  6. Xiong Y, et al. 2020. J Nanobiotechnology. 18:66. PubMed
  7. Cui J, et al. 2021. J Cell Mol Med. 25:5586. PubMed
  8. Zhang B, et al. 2022. Front Med (Lausanne). 8:796743. PubMed
  9. Wang HH, et al. 2022. J Biomed Sci. 29:36. PubMed
  10. Zhang Z, et al. 2022. Front Pharmacol. 13:906625. PubMed
  11. Yu L, et al. 2021. Cell Biol Int. 45:2452. PubMed
  12. Li H, et al. 2022. Cancer Cell. 40:36. PubMed
  13. Silveira DB, et al. 2022. Cell Biochem Funct. 40:163. PubMed
  14. Liang X, et al. 2022. Cancer Commun (Lond). 42:205. PubMed
  15. Lee R, et al. 2022. Cancer Discov. 12:1702. PubMed
  16. Wang S, et al. 2022. Sci Adv. 8:eabn3883. PubMed
  17. Li D, et al. 2022. Cell Prolif. 55:e13299. PubMed
  18. Li Y, et al. 2023. Bioact Mater. 20:548. PubMed
  19. Liu Z, et al. 2022. Cancer Commun (Lond). 42:971. PubMed
  20. Meng Z, et al. 2022. Int J Oncol. 61: . PubMed
  21. Zhu J, et al. 2022. Nat Commun. 13:7466. PubMed
  22. Li J, et al. 2023. Front Immunol. 13:945234. PubMed
  23. Choi H, et al. 2023. J Nanobiotechnology. 21:5. PubMed
  24. Domingos-Pereira S, et al. 2022. Int J Mol Sci. 24: . PubMed
  25. Li Y, et al. 2023. Cell Death Dis. 14:28. PubMed
  26. Zhang L, et al. 2023. J Inflamm Res. 16:109. PubMed
  27. Fan R, et al. 2023. J Immunother Cancer. 11: . PubMed
  28. Chun J, et al. 2023. Cancers (Basel). 15: . PubMed
  29. Li S, et al. 2023. Exp Mol Med. 55:457. PubMed
  30. Zhao X, et al. 2022. Cell Mol Immunol. 19:540. PubMed
  31. Lundahl MLE, et al. 2022. Elife. 11:. PubMed
  32. Adzika GK, et al. 2023. Front Immunol. 14:1124649. PubMed
  33. Bautista CA, et al. 2023. Front Physiol. 14:1122348. PubMed
  34. Garabuczi É, et al. 2023. Front Immunol. 14:1139204. PubMed
  35. Chung JY, et al. 2023. Nat Commun. 14:1794. PubMed
  36. Shi Y, et al. 2023. Nat Commun. 14:1884. PubMed
  37. Pan C, et al. 2023. Front Bioeng Biotechnol. 11:1129187. PubMed
  38. Levra Levron C, et al. 2023. Nat Cell Biol. 25:740. PubMed
  39. Seclì L, et al. 2023. J Immunother Cancer. 11:. PubMed
  40. Li T, et al. 2023. Nat Commun. 14:2498. PubMed
  41. Salemme V, et al. 2023. Nat Commun. 14:2350. PubMed
  42. Zeng S, et al. 2023. Front Oncol. 13:1171926. PubMed
  43. Tripathi H, et al. 2020. J Mol Cell Cardiol. 149:95. PubMed
  44. Hu S, et al. 2018. Front Immunol. 9:01. PubMed
  45. Ying W et al. 2017. Cell. 171(2):372-384 . PubMed
  46. Clarke F,et al. 2017. PLoS One. 10.1371/journal.pone.0186625. PubMed
  47. Zhang Y, et al. 2018. Sci Rep. 8:5994. PubMed
  48. Hsu SPC, et al. 2020. J Neurooncol. 146:417. PubMed
  49. Nomura M, et al. 2016. Nat Immunol. 17:216-217. PubMed
  50. Leach SM, et al. 2020. Cell Rep. 33:108337. PubMed
  51. Zheng W, et al. 2021. Wellcome Open Research. 6:52. PubMed
  52. Uzhachenko RV, et al. 2021. Cell Reports. 35(1):108944. PubMed
  53. Dong L, et al. 2021. Cancer Cell. . PubMed
  54. Sadek J, et al. 2021. EMBO Mol Med. 13:e13591. PubMed
  55. Li Q, et al. 2021. Front Immunol. 12:707784. PubMed
  56. De Vlaeminck Y, et al. 2020. Cancers (Basel). 12:. PubMed
  57. Li J, et al. 2021. Front Immunol. 12:649285. PubMed
  58. Krukowski K, et al. 2021. Sci Adv. 7:eabg6702. PubMed
  59. Pajarinen J, et al. 2021. J Biomed Mater Res A. 109:1512. PubMed
  60. Zheng H, et al. 2021. Frontiers in Cell and Developmental Biology. 9:641527. PubMed
  61. Shao H, et al. 2012. J Biol Chem. 287:35860. PubMed
  62. Zhang J, et al. 2021. J Leukoc Biol. 110:485. PubMed
  63. Tanner L, et al. 2022. Front Pharmacol. 13:899469. PubMed
  64. Hou H, et al. 2021. Front Cell Dev Biol. 9:737003. PubMed
  65. Johansen AZ, et al. 2022. Pharmaceutics. 14:. PubMed
  66. Jia X, et al. 2021. Front Cell Dev Biol. 9:685522. PubMed
  67. He C, et al. 2021. Oncoimmunology. 1897295:10. PubMed
  68. Tang TT, et al. 2020. Sci Adv. 6:eaaz0748. PubMed
  69. Mul Fedele ML, et al. 2020. Front Cell Infect Microbiol. 10:100. PubMed
  70. El–Achkar GA, et al. 2019. PLoS One. 14:e0216405. PubMed
  71. Giri J, et al. 2020. Cell Rep. 30:1923. PubMed
  72. Dan H, et al. 2020. Mol Oncol. 14:795. PubMed
  73. Kim JS, et al. 2020. Int J Oncol. 56:1405. PubMed
  74. McKee AM, et al. 2021. iScience. 24:103012. PubMed
  75. Zhang Y, et al. 2022. Nat Commun. 13:4553. PubMed
  76. Rao X, et al. 2022. Cell Death Dis. 13:891. PubMed
  77. Li J, et al. 2021. Cell Commun Signal. 19:89. PubMed
  78. Mehta AK, et al. 2021. Nat Cancer. 2:66. PubMed
  79. Jiao Y, et al. 2021. Crit Care. 25:356. PubMed
  80. Martins L, et al. 2020. Stem Cell Res Ther. 0.786805556. PubMed
  81. Farini A, et al. 2020. Front Physiol. 0.738194444. PubMed
  82. An W et al. 2019. British journal of pharmacology. 176(5):699-710 . PubMed
  83. Guérin MV, et al. 2019. Nat Commun. 10:4131. PubMed
  84. Liao T, et al. 2017. Front Immunol. 8:1334. PubMed
  85. Wang J, et al. 2021. Nat Commun. 12:6198. PubMed
  86. Lu X, et al. 2020. Sci Transl Med. 12:. PubMed
  87. Wei W, et al. 2022. mSystems. 7:e0046922. PubMed
  88. Wang Y, et al. 2022. Cell Death Dis. 13:748. PubMed
  89. Golden TN, et al. 2022. Front Pharmacol. 12:761496. PubMed
  90. Cai C, et al. 2021. Sci Rep. 11:13386. PubMed
  91. Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed
  92. Tacconi C, et al. 2021. Cell Reports. 35(2):108993. PubMed
  93. Lee DH, et al. 2019. PLoS One. 14:e0215727. PubMed
  94. Yu Y, et al. 2018. Mol Med Rep. 17:8212. PubMed
  95. Ruiz de Azua I, et al. 2017. J Clin Invest. 127:4148. PubMed
  96. Miura I, et al. 2021. Physiol Rep. 9:e14859. PubMed
  97. Li H, et al. 2021. Nat Commun. 12:7149. PubMed
  98. Germundson DL, et al. 2022. Front Allergy. 3:870513. PubMed
  99. Anderson AE, et al. 2022. NPJ Regen Med. 7:6. PubMed
  100. Shu Y, et al. 2020. Immunology. 160:345. PubMed
  101. Ying W, et al. 2016. Sci Rep. 6:20176. PubMed
  102. Sztwiertnia I, et al. 2020. PLoS One. 15:e0233789. PubMed
  103. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  104. Chu SY, et al. 2019. Nat Commun. 10:1524. PubMed
  105. Bhattacharya A, et al. 2018. Neuropsychopharmacology. 43:2586. PubMed
  106. Lu X, et al. 2012. Arterioscler Thromb Vasc Biol. 32:2358. PubMed
  107. Qiu N, et al. 2021. J Nanobiotechnology. 19:428. PubMed
  108. Yang C, et al. 2022. Nat Commun. 13:4866. PubMed
  109. Anderson-Baucum E, et al. 2021. Cell Metab. 33:1883. PubMed
  110. Stevenson ER, et al. 2022. J Pharmacol Exp Ther. 382:356. PubMed
  111. Wang X, et al. 2022. J Biol Chem. 298:101561. PubMed
  112. Lowe PP, et al. 2020. J Neuroinflammation. 0.913888889. PubMed
  113. Zhou K, et al. 2016. J Cereb Blood Flow Metab. 10.1177/0271678X16648712. PubMed
  114. Deng W, et al. 2015. Clin Immunol. . PubMed
  115. Lu SW, et al. 2020. Nat Commun. 3.660416667. PubMed
  116. Long L, et al. 2018. Bio Protoc. 8:e2815. PubMed
  117. Li C, et al. 2019. JCI Insight. 5. PubMed
  118. Asterholm I, et al. 2012. J Lipid Res. 53: 1254. PubMed
  119. Stridh L, et al. 2013. J Neurosci. 33:12401. PubMed
  120. Budai Z, et al. 2021. Cells. 10:. PubMed
  121. Zhu Q, et al. 2022. Atherosclerosis. 357:41. PubMed
  122. Di Lorenzo A, et al. 2022. Oncoimmunology. 11:2086752. PubMed
  123. Li Y, et al. 2020. Theranostics. 10:2897. PubMed
  124. Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
  125. Zhang W, et al. 2021. Front Immunol. 12:768435. PubMed
  126. Suzuki T, et al. 2017. Cell Rep. 18(8):2045-2057. PubMed
  127. Krukowski K, et al. 2018. Sci Rep. 8:7857. PubMed
  128. Ukah T, et al. 2017. J Immunol. 10.4049/jimmunol.1700410. PubMed
  129. Roediger B et al. 2018. Cell. 175(2):530-543 . PubMed
  130. Santopaolo M, et al. 2021. Front Immunol. 609406:12. PubMed
  131. Liu C, et al. 2022. Cell Death Discov. 8:269. PubMed
  132. Sears SM, et al. 2022. Kidney360. 3:818. PubMed
  133. Lau P, et al. 2022. Cell Mol Immunol. :. PubMed
  134. Liu YS, et al. 2021. Cancers (Basel). 13:. PubMed
  135. Budhu S, et al. 2021. Cell Reports. 34(2):108620. PubMed
  136. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  137. Browne A, et al. 2015. Cancer Res . 75: 2811 - 2821. PubMed
  138. Moreno M et al. 2014. The Journal of Neuroscience. 34(24):8175-85 . PubMed
  139. Zhang J, et al. 2020. J Cell Mol Med. 24:4547. PubMed
  140. Sadtler K, et al. 2017. Tissue Eng Part A. 23:1044. PubMed
  141. Li B et al. 2019. Cell reports. 26(10):2720-2737 . PubMed
  142. Morioka S, et al. 2018. Nature. 563:714. PubMed
  143. Mellal K, et al. 2019. Sci Rep. 9:12903. PubMed
  144. Yuan Y, et al. 2019. Clin Sci (Lond). 133:1759. PubMed
  145. Hong Y, et al. 2019. J Extracell Vesicles. 8:1670893. PubMed
  146. Al-Zaeed N, et al. 2021. Cell Death Dis. 12:611. PubMed
  147. Golabek A, et al. 2021. Exp Ther Med. 21:482. PubMed
  148. Yeini E, et al. 2021. Nat Commun. 12:1912. PubMed
  149. Xu GY, et al. 2022. Small. 18:e2107838. PubMed
  150. Cai T, et al. 2022. Front Immunol. 13:1009932. PubMed
  151. Lal JC, et al. 2021. Breast Cancer Res. 23:83. PubMed
  152. Guo S, et al. 2021. Chronic Dis Transl Med. 7:254. PubMed
  153. Zheng H, et al. 2021. Frontiers in Immunology. 12:645100. PubMed
  154. Chuan Li et al. 2017. Endocrinology. 158(9):2837-2847 . PubMed
  155. Wen HJ, et al. 2019. Cell Mol Gastroenterol Hepatol. 8:p173. PubMed
  156. Yuan M, et al. 2017. Hum Reprod. 32:94. PubMed
  157. Riopel M, et al. 2019. Mol Metab. 20:89. PubMed
  158. Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
RRID
AB_10895754 (BioLegend Cat. No. 141705)
AB_10895754 (BioLegend Cat. No. 141706)

Antigen Details

Structure
Type I transmembrane protein, 175 kD, C-type lectin superfamily
Distribution

Macrophages, dendritic cells, Langerhans cells, liver endothelial cells

Function
Pathogen recognition, endocytosis and phagocytosis, antigen presentation
Ligand/Receptor
Antigen containing mannose, fucose, or an N-acetyl glucosamine
Cell Type
Dendritic cells, Endothelial cells, Langerhans cells, Macrophages
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
CD Molecules
Antigen References

1. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501.
2. Apostolopoulos V, et al. 2001. Curr. Mol. Med. 1:469.
3. Burgdorf S, et al. 2006. J. Immunol. 176:6770.
4. McKenzie EJ, et al. 2007. J. Immunol. 178:4975.

Gene ID
17533 View all products for this Gene ID
UniProt
View information about CD206 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Why is mouse CD206 stained intracellularly and not via surface staining?

Typically, mouse CD206 surface level is relatively low under normal conditions and so intracellular staining protocol is required to get better signal.

Go To Top Version: 3    Revision Date: 04/04/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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