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PE anti-mouse H-2 Antibody

Pricing & Availability
Clone
M1/42 (See other available formats)
Regulatory Status
RUO
Other Names
Mouse major histocompatibility complex (MHC) H-2
Isotype
Rat IgG2a, κ
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Product Citations
publications
M1slash42_PE_102108.jpg
C57BL/6 splenocytes stained with M1/42 PE
  • M1slash42_PE_102108.jpg
    C57BL/6 splenocytes stained with M1/42 PE
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125505 25 µg 88€
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125506 100 µg 210€
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Description

The M1/42 antibody reacts with the H-2 MHC class I alloantigens expressed on nucleated cells from mice of the a, b, d, j, k, s, and u haplotypes (Stallcup, KC et al, 1981). MHC class I is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C57BL/10 spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for other applications.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References
  1. Stallcup KC, et al. 1981 J. Immunol. 127:923
  2. Pyzik M, et al. 2014. J Immunol. 193:6061. PubMed
Product Citations
  1. Sugimoto M, et al. 2022. DNA Res. 29: . PubMed
  2. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  3. Pyzik M, et al. 2014. J Immunol. 193:6061. PubMed
  4. Oyarce C, et al. 2018. Front Immunol. 8:1794. PubMed
  5. Bockerstett KA, et al. 2018. Int J Mol Sci. 19:E1096. PubMed
  6. Saunderson S and McLellan A. 2017. J Immunol. 10.4049/jimmunol.1601537. PubMed
RRID
AB_1227705 (BioLegend Cat. No. 125505)
AB_1227705 (BioLegend Cat. No. 125506)

Antigen Details

Structure
45kd heavy chain non-covalently associated with 12 kd beta-2 microglobulin
Distribution

All nucleated cells

Function
Present antigenic peptide to CD8+ cytotoxic T cells
Ligand/Receptor
MHC/peptide
Biology Area
Immunology
Molecular Family
MHC Antigens
Antigen References

1. Stallcup KC, et al. 1981. J. Immunol. 127:923

Gene ID
111364 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
H-2
Specificity Alt (DOES NOT SHOW ON TDS):
H-2
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about H-2 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11-30-2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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