Loading...

Alexa Fluor® 488 anti-mouse F4/80 Antibody

Pricing & Availability
Clone
BM8 (See other available formats)
Regulatory Status
RUO
Other Names
EMR1, Ly71
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
BM8_A488_F4slash80_Antibody_1_FC_060616
Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with BM8 Alexa Fluor® 488.
  • BM8_A488_F4slash80_Antibody_1_FC_060616
    Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with BM8 Alexa Fluor® 488.
  • BM8_A488_F4slash80_Antibody_2_IHCF_060616
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS plus 5% rat/mouse serum for 30 minutes at room temperature. Then the section was stained with 5 µg/ml of anti-mouse F4/80 (clone BM8) Alexa Fluor® 488 (green), anti-mouse/human CD45R/B220 (clone RA3-6B2) Brilliant Violet 510™ (blue), and anti-mouse CD3 (clone 17A2) Brilliant Violet 421™ (red) overnight at 4°C. The image was captured with a 10X objective.
  • BM8_A488_F4slash80_Antibody_3_092021.png
    Paraformaldehyde-fixed (1%), 500 µm-thick mouse spleen section was processed according to the Ce3D™ Tissue Clearing Kit protocol (Cat. No. 427701). The section was costained with anti-mouse F4/80 Antibody (clone BM8) Alexa Fluor® 488 at 5 µg/mL (green), anti-mouse I-A/I-E Antibody (clone M5/114.15.2) Alexa Fluor® 594 at 5 µg/mL (blue), and anti-mouse CD146 Antibody (clone ME-9F1) Alexa Fluor® 647 at 5 µg/mL (magenta). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
    Watch the video.
Compare all formats See Alexa Fluor® 488 spectral data See high resolution IHC data...
Cat # Size Price Quantity Check Availability Save
123119 25 µg 118€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
123120 100 µg 259€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

F4/80, also known as EMR1 or Ly71, is a 160 kD glycoprotein of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80 has been widely used as a murine macrophage marker. It is expressed on a majority of tissue macrophages, including macrophages in the lung, gut, peritoneal cavity, thymus, and red pulp of the spleen, Kupffer cells, Langerhans cells, microglia, and certain dendritic cells. It is not expressed on macrophages located in the T cell areas of the spleen, lymph node, or Peyer's patch. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for the induction of CD8+ T cells-mediated peripheral tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F, 3D IHC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 2.5 - 10 µg/ml. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, Western blotting, and spatial biology (IBEX)12,13.

Application References
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
  5. Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
  10. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
  11. Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Kretschmer I, et al. 2016. J Biol Chem. 291: 4091 - 4106. PubMed
  2. Ho J, et al. 2016. PLoS Biol. 14:e2000117. PubMed
  3. Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed
  4. Zhang Z, et al. 2022. Front Pharmacol. 13:906625. PubMed
  5. Hutami IR, et al. 2022. Oral Dis. 28:1157. PubMed
  6. Ozga AJ, et al. 2022. Immunity. 55:82. PubMed
  7. Dolfi B, et al. 2022. Cell Rep. 39:110949. PubMed
  8. El-Naccache DW, et al. 2022. Cell Rep. 40:111150. PubMed
  9. Hao J, et al. 2022. Cell Rep. 41:111804. PubMed
  10. Leonardi I, et al. 2022. Cell. 185:831. PubMed
  11. Lu L, et al. 2023. Adv Sci (Weinh). 10:e2206212. PubMed
  12. Ferriz M, et al. 2023. STAR Protoc. 4:102079. PubMed
  13. Graça FA, et al. 2023. Nat Commun. 14:2900. PubMed
  14. Gordan S, et al. 2020. Cell Reports. 29(10):3033-3046.e4.. PubMed
  15. Yan Q, et al. 2020. Commun Biol. 288:3. PubMed
  16. Dong Z, et al. 2013. PLoS One. 18:80364. PubMed
  17. Tan L, et al. 2022. Biochem Biophys Rep. 32:101351. PubMed
  18. Zhang BZ, et al. 2022. JCI Insight. 7:. PubMed
  19. Watanabe M et al. 2017. eLife. 6 pii: e26635. PubMed
  20. Bordignon P et al. 2019. Cell Rep. 28(9):2358-2372 . PubMed
  21. Carlock C, et al. 2013. Reproduction. 146:491. PubMed
  22. Hu HJ, et al. 2020. Cell Death Dis. 1.168055556. PubMed
  23. Ogbechi J, et al. 2022. Front Immunol. 13:1001956. PubMed
  24. Bussi C, et al. 2022. Nat Commun. 13:7338. PubMed
  25. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  26. Ooi JD, et al. 2019. Nat Commun. 10:3392. PubMed
  27. Bangs DJ, et al. 2022. Cell Rep. 38:110266. PubMed
  28. Klarquist J, et al. 2021. Cell Rep. 36:109591. PubMed
  29. Ohnishi A, et al. 2015. PLoS One. 10:117616. PubMed
  30. Galle E, et al. 2022. Genome Biol. 23:207. PubMed
  31. Brocq M, et al. 2014. J Immunol. 192:6120. PubMed
  32. Jenkins RW, et al. 2018. Cancer Discov. 8:196. PubMed
  33. Niu S, et al. 2016. J Immunol. 197: 3293 - 3301. PubMed
  34. Tedford K, et al. 2017. Nat Commun. . 10.1038/s41467-017-02482-4. PubMed
  35. Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
  36. Luan HH et al. 2019. Cell. 178(5):1231-1244 . PubMed
  37. Linde N, et al. 2018. Nat Commun. 9:21. PubMed
  38. Bretscher P, et al. 2015. EMBO Mol Med. 7:593. PubMed
  39. Wheeler R, Gull E 2015. Nat Commun. 6: 8964. PubMed
  40. Wongchana W, et al. 2015. J Immunol. 195: 5337 - 5346. PubMed
  41. Zhou Z, et al. 2022. Life (Basel). 12:. PubMed
  42. De Santi C, et al. 2022. Mol Ther Nucleic Acids. 29:643. PubMed
  43. Zhang C, et al. 2022. Nat Commun. 13:3468. PubMed
  44. Suresh MV, et al. 2019. JCI Insight. 4:e133103. PubMed
  45. Carlock C, et al. 2014. J Immunol. 193:161. PubMed
  46. De Simone G, et al. 2021. Immunity. :. PubMed
  47. He J, et al. 2019. J Clin Invest. 130. PubMed
  48. Li H, et al. 2019. Front Immunol. 2.205555556. PubMed
  49. Blriot C, et al. 2021. Immunity. :. PubMed
  50. Wang H, et al. 2016. PLoS One. 11:e0167307. PubMed
  51. Care A, et al. 2014. Biol Reprod. 91:60. PubMed
  52. Caruso R, et al. 2009. Gastroenterology. 136:2270. PubMed
  53. Tapia-Abellán A, et al. 2021. Sci Adv. 7:eabf4468. PubMed
  54. Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed
  55. Jamali A, et al. 2020. Cell Reports. 32(9):108099. PubMed
  56. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
  57. Geng T, et al. 2021. J Infect Dis. 223:2186. PubMed
  58. Nair S, et al. 2021. JCI Insight. 6:. PubMed
  59. Veremeyko T, et al. 2019. J Neurosci Res. 97:162. PubMed
  60. Mori Y, et al. 2014. Sci Rep. 4:6997. PubMed
  61. Liu Y, et al. 2022. iScience. 25:105121. PubMed
  62. Guo D, et al. 2022. Bone Res. 10:45. PubMed
  63. Matsumoto A, et al. 2021. iScience. 24:102839. PubMed
  64. Muri J, et al. 2020. eLife. 9:e53627.. PubMed
  65. Luck H, et al. 2019. Nat Commun. 10:3650. PubMed
  66. Cianciaruso C, et al. 2020. Cell Reports. 27(10):3062-3080.e11.. PubMed
  67. Seki N, et al. 2022. iScience. 25:104838. PubMed
  68. Zhu Q, et al. 2022. Atherosclerosis. 357:41. PubMed
  69. von Roemeling CA, et al. 2020. Nat Commun. 11:1508. PubMed
  70. Settelmeier S, et al. 2020. PLoS One. 15:e0233261. PubMed
  71. Antsiferova M, et al. 2017. EMBO Mol Med. 9(1):27-45. PubMed
  72. Qian Z, et al. 2022. Biomedicines. 10:. PubMed
  73. Chapelin F, et al. 2017. Sci Rep.. 10.1038/s41598-017-17669-4. PubMed
  74. Bian Z, et al. 2012. J Immunol. 188:844. PubMed
  75. Chen W, et al. 2016. Nat Commun. 7: 11302. PubMed
  76. Ipseiz N, et al. 2020. EMBO J. 39:e103454. PubMed
  77. Khoury MK, et al. 2021. JVS Vasc Sci. 1:123. PubMed
  78. Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
  79. Chakraborty K, et al. 2017. Nat Commun. 8:13944. PubMed
  80. Yi-Wei Lin, Emmanuel Montassier, Dan Knights 2016. Sci Rep. 6:38599. PubMed
  81. Liu Y, et al. 2022. Exp Anim. :. PubMed
  82. Lee HN, et al. 2022. JCI Insight. 7:. PubMed
  83. Zheng C, et al. 2021. J Extracell Vesicles. 10:e12109. PubMed
  84. Wang Y, et al. 2021. Nature. 599:308. PubMed
  85. Zhang B, et al. 2021. Nature. 599:471. PubMed
  86. Chmielewski M and Abken H 2017. Cell Rep.. 10.1016/j.celrep.2017.11.063. PubMed
  87. Wunderlich CM, et al. 2018. Nat Commun. 9:1646. PubMed
  88. Li X, et al. 2018. Cell Host Microbe. 24:847. PubMed
  89. Rahman H, et al. 2020. J Invest Dermatol. . PubMed
  90. Chirivi RGS, et al. 2020. Cell Mol Immunol. . PubMed
  91. Ghosh M, et al. 2021. Cancer Cell. 39(4):494-508.e5. PubMed
RRID
AB_893479 (BioLegend Cat. No. 123119)
AB_893479 (BioLegend Cat. No. 123120)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution

Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kupffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells

Function
Induction of immunological tolerance
Cell Type
Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org

Other Formats

View All F4/80 Reagents Request Custom Conjugation
Description Clone Applications
Brilliant Violet 605™ anti-mouse F4/80 BM8 FC
Purified anti-mouse F4/80 BM8 FC,IHC,WB
Biotin anti-mouse F4/80 BM8 FC,IHC
FITC anti-mouse F4/80 BM8 FC
PE anti-mouse F4/80 BM8 FC
PE/Cyanine5 anti-mouse F4/80 BM8 FC
PE/Cyanine7 anti-mouse F4/80 BM8 FC
APC anti-mouse F4/80 BM8 FC
APC/Cyanine7 anti-mouse F4/80 BM8 FC
Alexa Fluor® 488 anti-mouse F4/80 BM8 FC,IHC-F,3D IHC
Alexa Fluor® 647 anti-mouse F4/80 BM8 FC,IHC-F,3D IHC
Pacific Blue™ anti-mouse F4/80 BM8 FC
PerCP anti-mouse F4/80 BM8 FC
PerCP/Cyanine5.5 anti-mouse F4/80 BM8 FC
Alexa Fluor® 700 anti-mouse F4/80 BM8 FC
Brilliant Violet 421™ anti-mouse F4/80 BM8 FC,IHC-F,SB
Brilliant Violet 510™ anti-mouse F4/80 BM8 FC
Alexa Fluor® 594 anti-mouse F4/80 BM8 IHC-F
Brilliant Violet 785™ anti-mouse F4/80 BM8 FC
Purified anti-mouse F4/80 (Maxpar® Ready) BM8 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse F4/80 BM8 FC
Brilliant Violet 650™ anti-mouse F4/80 BM8 FC
Brilliant Violet 711™ anti-mouse F4/80 BM8 FC
APC/Fire™ 750 anti-mouse F4/80 BM8 FC
TotalSeq™-A0114 anti-mouse F4/80 BM8 PG
TotalSeq™-B0114 anti-mouse F4/80 BM8 PG
TotalSeq™-C0114 anti-mouse F4/80 BM8 PG
Spark YG™ 570 anti-mouse F4/80 BM8 IHC-F
KIRAVIA Blue 520™ anti-mouse F4/80 BM8 FC
Ultra-LEAF™ Purified anti-mouse F4/80 BM8 FC,IHC,WB
APC/Fire™ 810 anti-mouse F4/80 BM8 FC
Spark NIR™ 685 anti-mouse F4/80 BM8 FC
Spark Blue™ 550 anti-mouse F4/80 BM8 FC
Brilliant Violet 570™ anti-mouse F4/80 BM8 FC
Brilliant Violet 750™ anti-mouse F4/80 BM8 FC
PE/Fire™ 810 anti-mouse F4/80 BM8 FC
Spark Red™ 718 anti-mouse F4/80 (Flexi-Fluor™) BM8 FC
Spark Blue™ 574 anti-mouse F4/80 (Flexi-Fluor™) BM8 FC
Go To Top Version: 7    Revision Date: 09-20-2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account