Loading...

Alexa Fluor® 647 anti-mouse CD31 Antibody

Pricing & Availability
Clone
MEC13.3 (See other available formats)
Regulatory Status
RUO
Other Names
PECAM-1, EndoCAM
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
1-MEC13dot3_Alx647_080607
C57BL/6 mouse splenocytes stained with MEC13.3 Alexa Fluor® 647
  • 1-MEC13dot3_Alx647_080607
    C57BL/6 mouse splenocytes stained with MEC13.3 Alexa Fluor® 647
  • 2-MEC13dot3_A647_CD31_Antibody_2_050819
    Formalin-fixed, 300 micron-thick mouse kidney section was blocked, permeabilized and stained overnight with CD31 (clone MEC13.3) Alexa Fluor® 647 (red) at 5 µg/mL, Tubulin Beta 3 (TUBB3)(clone AA10) Alexa Fluor® 488 (green) at 2.5 µg/mL, and Podoplanin (clone Pmab-1) Alexa Fluor® 594 (blue) at 5 µg/mL, optically cleared, then analyzed at 230 µm imaging depth on a confocal microscope. Watch the video.
  • 3-MEC133_A647_CD31_Antibody_3D-IHC_092121.png
    Paraformaldehyde-fixed (4%), 500 μm-thick mouse kidney section was processed according to the Ce3DTM Tissue Clearing Kit protocol (cat. no. 427701). The section was costained with anti-mouse Podoplanin Antibody (clone PMab-1) Alexa Fluor® 594 at 5 µg/mL (green), and anti-mouse CD31 Antibody (clone MEC13.3) Alexa Fluor® 647 at 5 µg/mL (magenta) and counterstained with DAPI (blue). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 20X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
  • 4_Simon_Merz
    In-vivo mouse testis labeling of blood vessels with Alexa Fluor® 647 anti-mouse CD31 (red, clone MEC13.3). Animal perfused and sample dehydrated and cleared with ECi. Autofluorescence (green). Image generously submitted to the 2017 Cell Life Imaging Competition by Simon Merz from University of Duisburg-Essen.
  • 5_48_Mouse_Gut_EpCAM_CD8_CD31
    Confocal image of C57BL/6 mouse small intestine sample acquired using the IBEX method of highly multiplexed antibody-based imaging: EpCAM (magenta) in Cycle 1, CD8 (blue) in Cycle 1, and CD31 (green) in Cycle 2. Tissues were prepared using ~1% (vol/vol) formaldehyde and a detergent. Following fixation, samples are immersed in 30% (wt/vol) sucrose for cryoprotection. Images are courtesy of Drs. Andrea J. Radtke and Ronald N. Germain of the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
  • 6_51_Mouse_Gut_EpCAM_CD31_MHCII
    Confocal image of C57BL/6 mouse small intestine sample acquired using the IBEX method of highly multiplexed antibody-based imaging: EpCAM (blue) in Cycle 1, CD31 (magenta) in Cycle 2, and MHCII (green) in Cycle 3. Tissues were prepared using ~1% (vol/vol) formaldehyde and a detergent. Following fixation, samples are immersed in 30% (wt/vol) sucrose for cryoprotection. Images are courtesy of Drs. Andrea J. Radtke and Ronald N. Germain of the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
102515 25 µg 81€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
102516 100 µg 184€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1), EndoCAM, and gpIIa. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-to-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (αv β3 integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation, and angiogenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Polyoma middle T transformed EC line tEnd.1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
3D IHC - Verified

SB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µL volume. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (in the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, immunohistochemical staining1,5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)12,13.
Special Note: The antibody works well on acetone-fixed frozen sections as well as Zinc-fixed paraffin-embedded sections. It sometime works on formalin-fixed and paraformaldehyde-fixed paraffin-embedded tissue sections but inconsistent results have been reported. This antibody is not recommended for formalin-fixed paraffin-embedded sections or for Western blot analysis. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102529 and 102530).

Application References
  1. Vecchi A, et al. 1994. Eur. J. Cell Biol. 63:247. (IP, IHC, Block)
  2. Christofidou-Solomidou M, et al. 1997. J. Immunol. 158:4872. (Block)
  3. DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
  4. Rosenblum WI, et al. 1994. Am. J. Pathol. 145:33. (Block)
  5. Baldwin HS, et al. 1994. Development 120:2539. (IHC)
  6. Voswinckel R, et al. 2003. Circ. Res. 93:372. (IHC)
  7. Leung VW, et al. 2009. Am J. Pathol. 175:1757. PubMed
  8. Chacko AM, et al. 2012. PLoS One 7:e34958.
  9. Giacomini C, et al. 2014. Exp Eye Res. 18:1. PubMed
  10. Morita R, et al. 2015. PNAS. 112:160. PubMed
  11. Ito A, et al. 2015. Brain Res. 1594:310. PubMed
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Himburg HA et al. 2018. Cell stem cell. 23(3):370-381 . PubMed
  2. Neufert C, et al. 2020. Nature Protocols. 16(1):61-85. PubMed
  3. Sandovici I, et al. 2022. Dev Cell. 57:63. PubMed
  4. Daeschler SC, et al. 2022. Neural Regen Res. 17:1348. PubMed
  5. Zhang D, et al. 2022. Cell Stem Cell. 29:232. PubMed
  6. Schwarzenberg FL, et al. 2022. Front Immunol. 13:947961. PubMed
  7. Arnholdt C, et al. 2022. Cells. 11:. PubMed
  8. Cherief M, et al. 2022. Stem Cells Transl Med. 11:1165. PubMed
  9. Lütge M, et al. 2023. Nat Immunol. . PubMed
  10. Choudhury SR, et al. 2020. Cell. 178(5):1205-1221.e17.. PubMed
  11. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  12. Merz SF, et al. 2019. Nat Commun. 10:2312. PubMed
  13. Mason HD, et al. 2021. JCI Insight. 6:e149229. PubMed
  14. Götz P, et al. 2022. Int J Mol Sci. 23:. PubMed
  15. Silva C, et al. 2019. Cell Physiol Biochem. 52:503. PubMed
  16. Böttcher JP, et al. 2018. Cell. 172:1022. PubMed
  17. Kumaraswami K, et al. 2020. Int J Mol Sci. 21:00. PubMed
  18. Evren E, et al. 2020. Immunity. 54(2):259-275.e7. PubMed
  19. Perry HM, et al. 2018. J Am Soc Nephrol. 29:194. PubMed
  20. Ni F, et al. 2019. Cell Stem Cell. 24:608. PubMed
  21. Mariani SA, et al. 2019. Immunity. 50:1439. PubMed
  22. Jiang YH, et al. 2020. J Mol Cell Cardiol. 138:115. PubMed
  23. Perry H, et al. 2016. J Am Soc Nephrol. 10.1681/ASN.2015080922. PubMed
  24. Minatoguchi S, et al. 2022. Sci Rep. 12:5389. PubMed
  25. Wang C, et al. 2021. Cell Rep. 37:110021. PubMed
  26. Liu M, et al. 2020. Nature. 587:115. PubMed
  27. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  28. Kübler M, et al. 2021. Biomedicines. 9:. PubMed
  29. Kübler M, et al. 2021. Int J Mol Sci. 22:. PubMed
  30. Frii J, et al. 2021. Immunity. 54(5):1002-1021.e10. PubMed
  31. Formaglio P, et al. 2014. Parasitol Int. 63:273. PubMed
  32. Zotter B, et al. 2022. J Neurosci. 42:183. PubMed
  33. Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed
  34. Devi S, et al. 2021. Immunity. 54(6):1219-1230.e7. PubMed
  35. Shannon JP, et al. 2021. STAR Protoc. 2:100790. PubMed
  36. Pinho S et al. 2018. Developmental cell. 44(5):634-641 . PubMed
  37. Shannon JP, et al. 2021. Immunity. 54(2):276-290.e5. PubMed
  38. Lustgarten Guahmich N, et al. 2020. Angiogenesis. 23:443. PubMed
  39. Kübler M, et al. 2021. Int J Mol Sci. 22:. PubMed
  40. Stacy A, et al. 2021. Cell. 184(3):615-627.e17. PubMed
  41. Wei Q, et al. 2020. Dev Cell. 53:503. PubMed
  42. Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
  43. Cheng HW, et al. 2022. Nat Commun. 13:2027. PubMed
  44. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
  45. Wang S, et al. 2021. Commun Biol. 22:. PubMed
  46. Hofmann J, et al. 2021. Front Immunol. 11:599495. PubMed
  47. Qiao L, et al. 2014. Circulation. 129:629. PubMed
  48. Götz P, et al. 2021. Int J Mol Sci. 22: . PubMed
  49. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  50. Hsu KS, et al. 2022. Nat Commun. 13:7078. PubMed
  51. Collins N, et al. 2020. Cell. 178(5):1088-1101.e15.. PubMed
  52. Xu C, et al. 2018. Nat Commun. 9:2449. PubMed
  53. Rustenhoven J, et al. 2021. Cell. 184(4):1000-1016.e27. PubMed
  54. Tsukada Y, et al. 2021. Sci Rep. 0.975694444. PubMed
  55. Rodriguez AB, et al. 2022. Front Immunol. 13:1009306. PubMed
  56. Kim K, et al. 2022. Front Immunol. 13:844919. PubMed
  57. Gao X, et al. 2021. Nature. 589:591. PubMed
  58. Baron CS, et al. 2018. Nat Commun. 9:2517. PubMed
  59. Shimba A et al. 2018. Immunity. 48(2):286-298 . PubMed
  60. Xu M, et al. 2020. Cell. 178(6):1478-1492.e20.. PubMed
  61. Hoyer FF, et al. 2020. Immunity. 51(5):899-914.e7.. PubMed
  62. Courties G, et al. 2019. Circ Res. 124:1372. PubMed
  63. Winter C, et al. 2018. Cell Metab. 28:175. PubMed
  64. Klingberg A, et al. 2016. J Am Soc Nephrol. 10.1681/ASN.2016020232. PubMed
  65. Lasch M, et al. 2020. Front Physiol. 11:576736. PubMed
  66. Sakamoto K, et al. 2022. STAR Protoc. 3:101052. PubMed
  67. Lee EKS, et al. 2018. Cell Host Microbe. 23:121. PubMed
  68. Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed
  69. Asada N, et al. 2017. Nat Cell Biol. 19:214-223. PubMed
  70. Stanwick M, et al. 2022. Front Cell Dev Biol. 10:834815. PubMed
  71. Bas G J Surewaard et al. 2018. Cell host & microbe. 24(2):271-284 . PubMed
  72. Kikuchi H, et al. 2020. Cancer Res. 80:2996. PubMed
  73. Bryson J, et al. 2011. J Vis Exp. 56: 3267. PubMed
RRID
AB_2161029 (BioLegend Cat. No. 102515)
AB_2161029 (BioLegend Cat. No. 102516)

Antigen Details

Structure
Ig superfamily, 130-140 kD
Distribution

Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets

Function
Adhesion
Ligand/Receptor
CD38, αV/β3 integrin
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.

Gene ID
18613 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org

Related FAQs

If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?

It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.

Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?

Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.

Are other fluorophores compatible with IBEX?

Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.

The same antibody works in one tissue type but not another. What is happening?

Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.

How can I be sure the staining I’m seeing in my tissue is real?

In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.

Go To Top Version: 6    Revision Date: 09-21-2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account