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Brilliant Violet 421™ anti-human CD185 (CXCR5) Antibody

Pricing & Availability
Clone
J252D4 (See other available formats)
Regulatory Status
RUO
Other Names
BLR1, MDR15
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
J252D4_BV421_CD185_Antibody_FC_1_070313
Human peripheral blood lymphocytes were stained with CD19 Alexa Fluor® 488 and CD185 (clone J252D4) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • J252D4_BV421_CD185_Antibody_FC_1_070313
    Human peripheral blood lymphocytes were stained with CD19 Alexa Fluor® 488 and CD185 (clone J252D4) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • J252D4_BV421_CD185_Antibody_FC_2_070313
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
356919 25 tests 155€
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356920 100 tests 331€
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Description

CD185, also known as CXCR5, is a 42 kD G-protein coupled receptor with seven transmembrane regions. CXCR5 is expressed by mature B cells, follicular helper T cells, Burkitt’s lymphoma cells and a subset of neurons, and mediates cell migration to the B cell follicles in the secondary lymphoid organs. The ligand of CXCR5 is CXCL13 (BLC).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CXCR5-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Product Citations
  1. Hagel J, et al. 2021. J Immunol. 206:3073. PubMed
  2. Nayrac M, et al. 2022. Cell Rep. 39:111013. PubMed
  3. Nicolas A, et al. 2023. iScience. 26:105904. PubMed
  4. Goyal G, et al. 2022. Adv Sci (Weinh). 9:e2103241. PubMed
  5. Sannier G, et al. 2023. Cell Rep Med. 4:100955. PubMed
  6. Khanam A, et al. 2021. Front Immunol. 11:599648. PubMed
  7. Acharya A, et al. 2020. J Virol. 95:e01657. PubMed
  8. Grifoni A, et al. 2020. Cell. 181(7):1489-1501.e15.. PubMed
  9. Yang L, et al. 2020. Genes Dis. 7:128. PubMed
  10. Vikkurthi R, et al. 2022. Nat Microbiol. 7:974. PubMed
  11. Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
  12. Maehara T, et al. 2018. Life Sci Alliance. 1:e201800050. PubMed
  13. Rydyznski Moderbacher C, et al. 2020. Cell. 183(4):996-1012.e19. PubMed
  14. Rydyznski Moderbacher C, et al. 2022. J Clin Invest. :. PubMed
  15. Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed
  16. Tauzin A, et al. 2021. Cell Host Microbe. . PubMed
  17. Vanoni G, et al. 2021. eLife. 10:00. PubMed
  18. Högelin KA, et al. 2021. iScience. 24:103078. PubMed
  19. Gamradt S, et al. 2021. iScience. 24:103312. PubMed
  20. Paris R, et al. 2015. PLoS One. 10: 0144767. PubMed
RRID
AB_2562302 (BioLegend Cat. No. 356919)
AB_2562302 (BioLegend Cat. No. 356920)

Antigen Details

Structure
G-protein coupled receptor, 7 transmembrane regions, 42 kD
Distribution

Mature B cells, Follicular helper T cells, and Burkitt's lymphoma cells.

Function
Mediates cell migration to the B cell follicles in the secondary lymphoid organs
Ligand/Receptor
CXCL13
Cell Type
B cells, Tfh
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Ma CS, et al. 2012. J. Exp. Med. 209:1241.
2. León B, et al. 2012. Nat. Immunol. 13:681.
3. Crotty S. 2011. Annu. Rev. Immunol. 29:621.
4. Kerfoot SM, et al. 2011. Immunity 34:947.
5. Sáez de Guinoa J, et al. 2011. Blood 118:1560.

Gene ID
643 View all products for this Gene ID
UniProt
View information about CD185 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Other Formats

View All CD185 Reagents Request Custom Conjugation
Description Clone Applications
PE anti-human CD185 (CXCR5) J252D4 FC
APC anti-human CD185 (CXCR5) J252D4 FC
Purified anti-human CD185 (CXCR5) J252D4 FC
Alexa Fluor® 647 anti-human CD185 (CXCR5) J252D4 FC
PerCP/Cyanine5.5 anti-human CD185 (CXCR5) J252D4 FC
Alexa Fluor® 488 anti-human CD185 (CXCR5) J252D4 FC
FITC anti-human CD185 (CXCR5) J252D4 FC
Alexa Fluor® 700 anti-human CD185 (CXCR5) J252D4 FC
Pacific Blue™ anti-human CD185 (CXCR5) J252D4 FC
Brilliant Violet 421™ anti-human CD185 (CXCR5) J252D4 FC
PE/Cyanine7 anti-human CD185 (CXCR5) J252D4 FC
APC/Cyanine7 anti-human CD185 (CXCR5) J252D4 FC
PE/Dazzle™ 594 anti-human CD185 (CXCR5) J252D4 FC
Brilliant Violet 605™ anti-human CD185 (CXCR5) J252D4 FC
Biotin anti-human CD185 (CXCR5) J252D4 FC
Brilliant Violet 711™ anti-human CD185 (CXCR5) J252D4 FC
Brilliant Violet 785™ anti-human CD185 (CXCR5) J252D4 FC
TotalSeq™-A0144 anti-human CD185 (CXCR5) J252D4 PG
TotalSeq™-C0144 anti-human CD185 (CXCR5) J252D4 PG
Brilliant Violet 750™ anti-human CD185 (CXCR5) J252D4 FC
TotalSeq™-B0144 anti-human CD185 (CXCR5) J252D4 PG
APC/Fire™ 750 anti-human CD185 (CXCR5) J252D4 FC
Spark NIR™ 685 anti-human CD185 (CXCR5) J252D4 FC
TotalSeq™-D0144 anti-human CD185 (CXCR5) J252D4 PG
PE/Cyanine5 anti-human CD185 (CXCR5) J252D4 FC
PE/Fire™ 700 anti-human CD185 (CXCR5) Antibody J252D4 FC
APC/Fire™ 810 anti-human CD185 (CXCR5) Antibody J252D4 FC
PerCP/Fire™ 806 anti-human CD185 (CXCR5) J252D4 FC
PerCP/Fire™ 780 anti-human CD185 (CXCR5) J252D4 FC
Spark Red™ 718 anti-human CD185 (CXCR5) J252D4 FC
APC anti-human CD185 J252D4 FC
Brilliant Violet 510™ anti-human CD185 (CXCR5) J252D4 FC
Brilliant Violet 650™ anti-human CD185 (CXCR5) J252D4 FC
Go To Top Version: 1    Revision Date: 07-16-2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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