Brilliant Violet 650™ anti-human CD185 (CXCR5) Antibody, CD185, J252D4

Pricing & Availability

Clone: J252D4 (See other available formats)
Regulatory Status: RUO
Other Names: BLR1, MDR15
Isotype: Mouse IgG1, κ
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Product Citations: publications

J252D4_BV-650_CD195_FC_072524 — Human peripheral blood lymphocytes were stained with anti-human CD19 (clone HIB19) FITC and anti-human CD185 (CXCR5) (clone J252D4) Brilliant Violet 650™ (left) or mouse IgG1, κ Brilliant Violet 650™ isotype control (right).

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356965

25 tests

177€

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356966

100 tests

348€

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Description

CD185, also known as CXCR5, is a 42 kD G-protein coupled receptor with seven transmembrane regions. CXCR5 is expressed by mature B cells, follicular helper T cells, Burkitt’s lymphoma cells and a subset of neurons, and mediates cell migration to the B cell follicles in the secondary lymphoid organs. The ligand of CXCR5 is CXCL13 (BLC).

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CXCR5-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
RRID: AB_3662266 (BioLegend Cat. No. 356965)
AB_3662266 (BioLegend Cat. No. 356966)

Antigen Details

Structure: G-protein coupled receptor, 7 transmembrane regions, 42 kD
Distribution: Mature B cells, Follicular helper T cells, and Burkitt's lymphoma cells.
Function: Mediates cell migration to the B cell follicles in the secondary lymphoid organs
Ligand/Receptor: CXCL13
Cell Type: B cells, Tfh
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Ma CS, et al. 2012. J. Exp. Med. 209:1241.
2. León B, et al. 2012. Nat. Immunol. 13:681.
3. Crotty S. 2011. Annu. Rev. Immunol. 29:621.
4. Kerfoot SM, et al. 2011. Immunity 34:947.
5. Sáez de Guinoa J, et al. 2011. Blood 118:1560.
Gene ID: 643 View all products for this Gene ID
UniProt: View information about CD185 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Pathways

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Other Formats

View All CD185 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-human CD185 (CXCR5)	J252D4	FC
APC anti-human CD185 (CXCR5)	J252D4	FC
Purified anti-human CD185 (CXCR5)	J252D4	FC
Alexa Fluor® 647 anti-human CD185 (CXCR5)	J252D4	FC
PerCP/Cyanine5.5 anti-human CD185 (CXCR5)	J252D4	FC
Alexa Fluor® 488 anti-human CD185 (CXCR5)	J252D4	FC
FITC anti-human CD185 (CXCR5)	J252D4	FC
Alexa Fluor® 700 anti-human CD185 (CXCR5)	J252D4	FC
Pacific Blue™ anti-human CD185 (CXCR5)	J252D4	FC
Brilliant Violet 421™ anti-human CD185 (CXCR5)	J252D4	FC
PE/Cyanine7 anti-human CD185 (CXCR5)	J252D4	FC
APC/Cyanine7 anti-human CD185 (CXCR5)	J252D4	FC
PE/Dazzle™ 594 anti-human CD185 (CXCR5)	J252D4	FC
Brilliant Violet 605™ anti-human CD185 (CXCR5)	J252D4	FC
Biotin anti-human CD185 (CXCR5)	J252D4	FC
Brilliant Violet 711™ anti-human CD185 (CXCR5)	J252D4	FC
Brilliant Violet 785™ anti-human CD185 (CXCR5)	J252D4	FC
TotalSeq™-A0144 anti-human CD185 (CXCR5)	J252D4	PG
TotalSeq™-C0144 anti-human CD185 (CXCR5)	J252D4	PG
Brilliant Violet 750™ anti-human CD185 (CXCR5)	J252D4	FC
TotalSeq™-B0144 anti-human CD185 (CXCR5)	J252D4	PG
APC/Fire™ 750 anti-human CD185 (CXCR5)	J252D4	FC
Spark NIR™ 685 anti-human CD185 (CXCR5)	J252D4	FC
TotalSeq™-D0144 anti-human CD185 (CXCR5)	J252D4	PG
PE/Cyanine5 anti-human CD185 (CXCR5)	J252D4	FC
PE/Fire™ 700 anti-human CD185 (CXCR5) Antibody	J252D4	FC
APC/Fire™ 810 anti-human CD185 (CXCR5) Antibody	J252D4	FC
PerCP/Fire™ 806 anti-human CD185 (CXCR5)	J252D4	FC
PerCP/Fire™ 780 anti-human CD185 (CXCR5)	J252D4	FC
Spark Red™ 718 anti-human CD185 (CXCR5)	J252D4	FC
APC anti-human CD185	J252D4	FC
Brilliant Violet 510™ anti-human CD185 (CXCR5)	J252D4	FC
Brilliant Violet 650™ anti-human CD185 (CXCR5)	J252D4	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 06-14-2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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