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PE anti-human/rat CD51 (Integrin αvβ5) Antibody

Pricing & Availability
Clone
P1F6 (See other available formats)
Regulatory Status
RUO
Other Names
ITGAV, MSK8, VNRA, VTNR
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P1F6_PE_CD51_Antibody_090716
Human fibrocarcinoma cell line HT1080 was stained with CD51 (clone P1F6) PE (closed histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • P1F6_PE_CD51_Antibody_090716
    Human fibrocarcinoma cell line HT1080 was stained with CD51 (clone P1F6) PE (closed histogram) or mouse IgG1, κ PE isotype control (open histogram).
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920007 25 tests 108€
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920008 100 tests 240€
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Description

CD51 is a type I integral membrane glycoprotein, known as vitronectin receptor α chain, or integrin αV. It forms heterodimer with integrin β1 (CD29), β3 (CD61), β5, β6, or β8. CD51 contains two disulfide-linked subunits of 125 kD and 24 kD, and is expressed on endothelial cells, fibroblasts, macrophages, platelets, osteoclasts, neuroblastoma, melanoma, and hepatoma cells. Many extracellular matrix proteins with RGD-motifs are CD51 ligands. In association with its β chains, CD51 binds vitronectin, von Willebrand factor, fibronectin, thrombospondin, osteopontin, fibrinogen, and laminin. CD51, as an adhesion molecule, plays important roles in leukocytes homing and rolling and mediates bone absorption and angiogenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Rat
Reported Reactivity
Hamster
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
The P1F6 monoclonal antibody was generated against human small cell lung carcinoma cells (UCLA P3).
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

P1F6, reacts with the avß5 integrin complex. This antibody does not cross react with any other av containing integrin and completely inhibits avß5 dependent binding to vitronectin coated surfaces.

Product Citations
  1. Hoeeg C, et al. 2021. Curr Protoc. 1:e40. PubMed
  2. Stepanenko AA, et al. 2021. Sci Rep. 11:16088. PubMed
  3. Stepanenko AA, et al. 2022. Mol Ther Oncolytics. 24:230. PubMed
RRID
AB_2629779 (BioLegend Cat. No. 920007)
AB_2629779 (BioLegend Cat. No. 920008)

Antigen Details

Structure
Two disulfide-linked subunits of 125 kD and 24 kD.
Distribution

Endothelial cells, fibroblasts, macrophages, platelets, osteoclasts, neuroblastoma, melanoma, and hepatoma cells.

Function
As an adhesion molecule, it plays important roles in leukocytes homing and rolling as well as mediates bone absorption and angiogenesis.
Interaction
Forms heterodimer with integrin β1 (CD29), β3 (CD61), β5, β6, or β8.
Cell Type
Endothelial cells, Fibroblasts, Macrophages, Mesenchymal Stem Cells, Osteoclasts, Platelets
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Burvenich I, et al. 2008. Mol. Cancer Ther. 7(12):3771.
2. Lee P, et al. 2014. J. Biol. Chem. 289(3):1467.
3. Su G, et al. 2007. Am. J. Respir. Cell Mol. Biol. 36(3):377.
4. Wayner EA, et al. 2007. Methods in Enzymology 426:117.
5. Weinacker A, et al. 1994. J. Biol. Chem. 269(9):6940.

Gene ID
3685 View all products for this Gene ID
UniProt
View information about CD51 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 09-08-2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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