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Purified anti-human/rat CD51 (Integrin αvβ5) Antibody

Pricing & Availability
Clone
P1F6 (See other available formats)
Regulatory Status
RUO
Other Names
ITGAV, MSK8, VNRA, VTNR
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P1F6_PURE_CD51_Antibody_030816
HT1080 cells were stained with purified CD51 (clone P1F6) (closed histogram), or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • P1F6_PURE_CD51_Antibody_030816
    HT1080 cells were stained with purified CD51 (clone P1F6) (closed histogram), or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
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920004 100 µg 90€
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Description

CD51 is a type I integral membrane glycoprotein, known as vitronectin receptor α chain, or integrin αV. It forms heterodimer with integrin β1 (CD29), β3 (CD61), β5, β6, or β8. CD51 contains two disulfide-linked subunits of 125 kD and 24 kD, and is expressed on endothelial cells, fibroblasts, macrophages, platelets, osteoclasts, neuroblastoma, melanoma, and hepatoma cells. Many extracellular matrix proteins with RGD-motifs are CD51 ligands. In association with its β chains, CD51 binds vitronectin, von Willebrand factor, fibronectin, thrombospondin, osteopontin, fibrinogen, and laminin. CD51, as an adhesion molecule, plays important roles in leukocytes homing and rolling and mediates bone absorption and angiogenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Rat
Reported Reactivity
Hamster
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
The P1F6 monoclonal antibody was generated against human small cell lung carcinoma cells (UCLA P3).
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.50 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

P1F6, reacts with the avß5 integrin complex. This antibody does not cross react with any other av containing integrin and completely inhibits avß5 dependent binding to vitronectin coated surfaces.

Product Citations
  1. Rothfelder K, et al. 2018. Neoplasia. 1150:20. PubMed
  2. Kayhan M, et al. 2019. J Immunol. 3247:203. PubMed
RRID
AB_2571949 (BioLegend Cat. No. 920004)

Antigen Details

Structure
Two disulfide-linked subunits of 125 kD and 24 kD.
Distribution

Endothelial cells, fibroblasts, macrophages, platelets, osteoclasts, neuroblastoma, melanoma, and hepatoma cells.

Function
As an adhesion molecule, it plays important roles in leukocytes homing and rolling as well as mediates bone absorption and angiogenesis.
Interaction
Forms heterodimer with integrin β1 (CD29), β3 (CD61), β5, β6, or β8.
Cell Type
Endothelial cells, Fibroblasts, Macrophages, Mesenchymal Stem Cells, Osteoclasts, Platelets
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Burvenich I, et al. 2008. Mol. Cancer Ther. 7(12):3771.
2. Lee P, et al. 2014. J. Biol. Chem. 289(3):1467.
3. Su G, et al. 2007. Am. J. Respir. Cell Mol. Biol. 36(3):377.
4. Wayner EA, et al. 2007. Methods in Enzymology 426:117.
5. Weinacker A, et al. 1994. J. Biol. Chem. 269(9):6940.

Gene ID
3685 View all products for this Gene ID
UniProt
View information about CD51 on UniProt.org
Go To Top Version: 3    Revision Date: 08-10-2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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