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PE anti-mouse CD209a (DC-SIGN) Antibody

Pricing & Availability
Clone
MMD3 (See other available formats)
Regulatory Status
RUO
Other Names
CD209 antigen-like protein A, dendritic cell-specific ICAM-3-grabbing non-integrin, CIRE, CD209, CDSIGN
Isotype
Mouse IgG2c, κ
Ave. Rating
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Product Citations
publications
MMD3_PE_CD209_Antibody_1_080421
C57BL/6 peritoneal macrophages were first treated with TruStain fcX™ (anti-mouse CD16/32) Antibody and True-Stain Monocyte Blocker™. Cells were then stained with PE anti-mouse CD209a (clone MMD3) (above) or PE mouse IgG2a, κ isotype control (below) and F4/80 (clone BM8) Alexa Fluor® 647. Data shown was derived from CD3/CD19/NK-1.1 negative populations.
  • MMD3_PE_CD209_Antibody_1_080421
    C57BL/6 peritoneal macrophages were first treated with TruStain fcX™ (anti-mouse CD16/32) Antibody and True-Stain Monocyte Blocker™. Cells were then stained with PE anti-mouse CD209a (clone MMD3) (above) or PE mouse IgG2a, κ isotype control (below) and F4/80 (clone BM8) Alexa Fluor® 647. Data shown was derived from CD3/CD19/NK-1.1 negative populations.
  • MMD3_PE_CD209_Antibody_3_122717
    CHO-DC-SIGN transfected (filled histogram) and CHO-K1 (dashed histogram) cell lines were stained with PE anti-mouse CD209a (clone MMD3) antibody.
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833003 25 µg 104€
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833004 100 µg 273€
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Description

CD209a, is also known as dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) and CIRE. Of the eight DC-SIGN-related receptors in mice, CD209a is considered the closest homolog to human DC-SIGN (CD209). Although both mouse and human counterparts are expressed on monocyte-derived dendritic cells, expression variation has been noted. Mouse CD209a is found on skin and splenic myeloid cells and is highly expressed on plasmacytoid dendritic cells, while human CD209 is expressed on myeloid dendritic cells, placental macrophages, liver and placental endothelial cells. CD209 binds to ICAM-3 (CD50), ICAM-2 (CD102), and Butyrophilin (BTN2A1), and mediates dendritic cell migration and T cell proliferation. It plays a critical role in capturing and internalizing pathogens such as Streptococcus pneumoniae.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a purified extracellular domain of mouse CD209a (DC-SIGN)-IgG1 Fc fusion protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 μg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The antibody will bind to both C57 and Balb/C mouse tissues.

Product Citations
  1. González-Ríos N, et al. 2023. J Mater Chem B. . PubMed
  2. Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
  3. Rivera CA, et al. 2022. Immunity. 55:129. PubMed
  4. Dyer DP et al. 2019. Immunity. 50(2):378-389 . PubMed
RRID
AB_2721637 (BioLegend Cat. No. 833003)
AB_2721637 (BioLegend Cat. No. 833004)

Antigen Details

Biology Area
Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Cai M, et al. 2016. Clin. Exp. Immunol. 317-25.
2. Ponichtera HE, et al. 2014. J. Immunol. 4655-65.

Gene ID
170786 View all products for this Gene ID
UniProt
View information about CD209a on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 3    Revision Date: 06-13-2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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