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PE/Cyanine7 anti-mouse CD49b Antibody

Pricing & Availability
Clone
HMα2 (See other available formats)
Regulatory Status
RUO
Other Names
α2 integrin, VLA-2 α chain, Integrin α2 chain, ITGA2, HMa2
Isotype
Armenian Hamster IgG
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Product Citations
publications
HMa2_PECyanine7_CD49b_Antibody_1_111723
C57BL/6 splenocytes were stained with NK1.1 APC and CD49b (clone HMα2) PE/Cyanine7 (left), or Armenian Hamster IgG PE/Cyanine7 isotype control (right).
  • HMa2_PECyanine7_CD49b_Antibody_1_111723
    C57BL/6 splenocytes were stained with NK1.1 APC and CD49b (clone HMα2) PE/Cyanine7 (left), or Armenian Hamster IgG PE/Cyanine7 isotype control (right).
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
103517 25 µg 90€
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103518 100 µg 268€
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Description

CD49b is a 150 kD glycoprotein, also known as α2 integrin, VLA-2 α chain, Integrin α2 chain, and HMα2. It is a member of the integrin family, expressed on NK cells, a subset of splenic CD4+ T cells, NK-T cells, intestinal intraepithelial and lamina propria lymphocytes, epithelial cells, and platelets. By associating with CD29 (integrin β1 subunit), CD49b forms the VLA-2 (integrin α2β1 ) complex. It plays a critical role in both adhesion and lymphocyte activation. The primary ligands for CD49b are collagen, laminin, and fibronectin. The HMα2 antibody has been shown to be useful for partially blocking CD49b mediated interactions with collagen. Additionally, this antibody blocks staining of splenic NK cells by the monoclonal antibody DX5.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse colon carcinoma cell line Colon26
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence on frozen sections4, and blocking of cell adhesion1-3. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 103525 & 103526).

Application References
  1. Miyake S, et al. 1994. Eur. J. Immunol. 24:2000. (Block, IP)
  2. Noto K, et al. 1995. Int. Immunol. 7:835. (Block)
  3. Arase H, et al. 2001. J. Immunol. 167:1141. (Block)
  4. Zhang Z, et al. 2008. Blood 111:1980. (IF)
Product Citations
  1. Edwards CL, et al. 2023. J Clin Invest. 133: . PubMed
  2. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  3. Hartmann W, et al. 2020. Cell Reports. 29(8):2243-2256.e4.. PubMed
  4. Hildreth AD, et al. 2020. STAR Protoc. 1:100113. PubMed
  5. Baker GJ, et al. 2020. Cell Syst. 0.647222222. PubMed
RRID
AB_2566103 (BioLegend Cat. No. 103517)
AB_2566103 (BioLegend Cat. No. 103518)

Antigen Details

Structure
Integrin family, associates with CD29 (β1 integrin), 150 kD
Distribution

NK cells, subset splenic CD4+ T cells, NK-T cells, intestinal intraepithelial and lamina propria lymphocytes, epithelial cells, platelets

Function
Adhesion, lymphocyte activation
Ligand/Receptor
Collagen, laminin, fibronectin
Cell Type
Epithelial cells, Lymphocytes, NK cells, NKT cells, Platelets, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Sasaki K, et al. 2003. Int. Immunol. 15:701.
3. Inoue O, et al. 2003. J. Cell Biol. 160:769.

Gene ID
16398 View all products for this Gene ID
UniProt
View information about CD49b on UniProt.org
Go To Top Version: 1    Revision Date: 07-20-2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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