- Clone
- 9E9 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- FcgRIV, Fcgr4, Fcrl3, CD16-2, FcgRIV, Fcgr3a, FcgammaRIV, 4833442P21Rik, Fc gamma RIV
- Isotype
- Armenian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1_9E9_Purified_CD16.2_Antibody_FC_010815 1_9E9_Purified_CD16.2_Antibody_FC_010815](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/9E9_Purified_CD16.2_Antibody_FC_010815.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 bone marrow cells were stained with purified CD16.2 (clone 9E9, filled histogram) or Armenian hamster IgG isotype control (open histogram), followed by anti-Armenian hamster IgG PE. Histograms are gated on the myeloid population. -
C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of CD16.2 (clone 9E9) purified, B220 (clone RA3-6B2) Alexa Fluor® 488 (blue) and CD3 (clone 17A2) Alexa Fluor® 647 (green) overnight at 4°C, followed by 2.5 µg/ml of anti-hamster IgG (clone Poly4055) Alexa Fluor® 594 (red) for two hours at room temperature.The image was captured with a 10X objective. -
C57BL/6 mouse frozen intestine section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of CD16.2 (clone 9E9) purified and CD324 (clone DECMA-1) Alexa Fluor® 647 (green) overnight at 4°C, followed by 2.5 µg/ml of anti-hamster IgG (clone Poly4055) Alexa Fluor® 594 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
149502 | 100 µg | 193€ |
FcγRIV, also known as CD16.2, is an intermediate-affinity activating receptor for IgG2a and IgG2b. CD16.2 is the mouse homolog of human FcγRIIIA. CD16.2 is a low-affinity IgE receptor for all allotypes and the ligation of FcγRIV by antigen-IgE immune complexes promotes macrophage-mediated phagocytosis and is involved in lung inflammation.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- FCγR4 —EC domain fusion with IgG1 Fc
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats of this clone) include: blocking of Fc?RIV function1 and inhibition of immune complex binding1,2. The LEAF™ or Ultra-LEAF™ purified antibody (Endotoxin < EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (contact our custom solutions team).
- Application References
-
- Mancardi DA, et al. 2008. J. Clin. Invest 118:3738. (FC, Block)
- Nimmerjahn F, et al. 2005. Immunity 23:41.
- Product Citations
-
- RRID
-
AB_2565302 (BioLegend Cat. No. 149502)
Antigen Details
- Structure
- 22.4 kD
- Distribution
-
Expressed on peripheral blood leukocytes, spleen, colon, bone marrow, and the intestine
- Function
- Activating receptor for IgG2a and IgG2b via association with an ITAM bearing accessory.
- Ligand/Receptor
- IgG2a and IgG2b
- Cell Type
- B cells, Leukocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Fc Receptors
- Antigen References
-
- Mechetina LV, et al. 2002. Immunogenetics. 54:463-8.
- Nimmerjahn F, et al. 2005. Immunity. 23:41-51.
- Seeling M, et al. 2013. Proc. Natl. Acad. Sci. 110:10729-34.
- Gene ID
- 246256 View all products for this Gene ID
- UniProt
- View information about CD16.2 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All CD16.2 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD16.2 (FcγRIV) | 9E9 | FC,IHC-F |
PE anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
APC anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
Biotin anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
PE/Cyanine7 anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
PE/Dazzle™ 594 anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
FITC anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
Brilliant Violet 421™ anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
Alexa Fluor® 488 anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
Alexa Fluor® 647 anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
Brilliant Violet 711™ anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
Brilliant Violet 785™ anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
Brilliant Violet 650™ anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
Brilliant Violet 510™ anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
APC/Cyanine7 anti-mouse CD16.2 (FcγRIV) | 9E9 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD16.2 (FcγRIV)
C57BL/6 bone marrow cells were stained with purified CD16.2 ... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... C57BL/6 mouse frozen intestine section was fixed with 4% par... -
PE anti-mouse CD16.2 (FcγRIV)
C57BL/6 bone marrow cells were stained with CD16.2 (clone 9E... -
APC anti-mouse CD16.2 (FcγRIV)
C57BL/6 bone marrow cells were stained with CD16.2 (clone 9E... -
Biotin anti-mouse CD16.2 (FcγRIV)
C57BL/6 mouse bone marrow cells were stained with biotinylat... -
PE/Cyanine7 anti-mouse CD16.2 (FcγRIV)
C57BL/6 mouse bone marrow cells were stained with CD16.2 (cl... -
PE/Dazzle™ 594 anti-mouse CD16.2 (FcγRIV)
C57BL/6 mouse bone marrow cells were stained with CD16.2 (cl... -
FITC anti-mouse CD16.2 (FcγRIV)
C57BL/6 mouse bone marrow cells were stained with CD16.2 (cl... -
Brilliant Violet 421™ anti-mouse CD16.2 (FcγRIV)
C57BL/6 mouse bone marrow cells were stained with CD16.2 (cl... -
Alexa Fluor® 488 anti-mouse CD16.2 (FcγRIV)
C57BL/6 bone marrow cells were stained with Alexa Fluor... -
Alexa Fluor® 647 anti-mouse CD16.2 (FcγRIV)
C57BL/6 bone marrow cells were stained with CD16.2 (clone 9E... -
Brilliant Violet 711™ anti-mouse CD16.2 (FcγRIV)
C57BL/6 mouse bone marrow cells were stained with CD16.2 (cl... -
Brilliant Violet 785™ anti-mouse CD16.2 (FcγRIV)
C57BL/6 mouse bone marrow cells were stained with CD16.2 (cl... -
Brilliant Violet 650™ anti-mouse CD16.2 (FcγRIV)
C57BL/6 mouse bone marrow cells were stained with CD16.2 (cl... -
Brilliant Violet 510™ anti-mouse CD16.2 (FcγRIV)
C57BL/6 mouse bone marrow cells were stained with CD16.2 (cl... -
APC/Cyanine7 anti-mouse CD16.2 (FcγRIV)
C57BL/6 myeloid cells were stained with anti-mouse CD16.2 (c...
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