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Spark Blue™ 574 anti-mouse CX3CR1 (Flexi-Fluor™) Antibody

Pricing & Availability
Clone
SA011F11 (See other available formats)
Regulatory Status
RUO
Other Names
Chemokine (C-X3-C motif) receptor 1, Fractalkine receptor, GPR13, CCRL1, CMKBRL1, CMKDR1, V28
Isotype
Mouse IgG2a, κ
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Cat # Size Price Quantity Save
285185 50 µg 269€
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Description

CX3CR1 is a 40 kD, G-protein coupled receptor, with seven transmembrane regions. CX3CR1 is expressed by resident and alternatively activated macrophages (M2), a subset of monocytes, dendritic cells (DCs), NK cells, a subset of memory T cells, and mast cells. CX3CR1 is involved in cell recruitment during inflammation and participates in cell adhesion and extravasation from blood vessels. Its ligand is CX3CL1, also known as fractalkine or neurotactin. CX3CR1 is also a coreceptor for HIV1 and variations in this gene leads to increased susceptibility to HIV. In the brain, it is expressed by glial cells, which interact with CX3CL1 expressed by neurons.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse CX3CR1-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 574 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC
Recommended Usage

Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

Excitation Laser
Blue Laser (488 nm)
Application Notes

View more applications data for a different format of this clone in our Scientific Poster Library.

Additional Product Notes

For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.

RRID
AB_3662525 (BioLegend Cat. No. 285185)

Antigen Details

Structure
G-protein coupled receptor, 7 transmembrane regions, 40 kD.
Distribution

Resident and alternatively activated macrophages (M2), subset of monocytes, dendritic cells (DCs), natural killer cells (NK), subset of memory T cells, mast cells, glial cells, and astrocytes.

Function
Involved in cell recruitment during inflammation, participates in cell adhesion and extravasation from blood vessels, coreceptor for HIV1.
Ligand/Receptor
CX3CL1 (fractalkine, neurotactin).
Cell Type
Astrocytes, Dendritic cells, Macrophages, Mast cells, Microglia, Monocytes, NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Ponzetta A, et al. 2013. J. Immunol. 191:5684.
2. Jacquelin S, et al. 2013. Blood. 122:674.
3. Garcia JA, et al. 2013. J. Immunol. 191:1063.
4. Lee YS, et al. 2013. Cell. 153:413.
5. Shechter R, et al. 2013. Immunity. 38:555.

Gene ID
NA
UniProt
View information about CX3CR1 on UniProt.org

Related FAQs

What are Flexi-Fluors?

Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer. 

How quickly will I receive my order?

We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary. 

How are Flexi-Fluors different from regular catalog products?

Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.

How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?

Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL. 

 

For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody.  Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. 

I can’t find the antibody-dye combination that I need. When will it be available?

We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services. 

I need help to validate the performance of my Flexi-Fluor. Who should I contact?

Please get in touch with Technical Service for assistance. 

Can I order more than 50 μg of a Flexi-Fluor?

Yes, you can order multiple vials of the same Flexi-Fluor products.  We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.

What is the expiration date of my Flexi-Fluor?

Expiration dates can be found on the vial label or by using our CoA lookup tool. 

Other Formats

View All CX3CR1 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse CX3CR1 SA011F11 FC
Alexa Fluor® 647 anti-mouse CX3CR1 SA011F11 FC
PE anti-mouse CX3CR1 SA011F11 FC
APC anti-mouse CX3CR1 SA011F11 FC
PerCP/Cyanine5.5 anti-mouse CX3CR1 SA011F11 FC
Brilliant Violet 421™ anti-mouse CX3CR1 SA011F11 FC
Brilliant Violet 510™ anti-mouse CX3CR1 SA011F11 FC
FITC anti-mouse CX3CR1 SA011F11 FC
Alexa Fluor® 488 anti-mouse CX3CR1 SA011F11 FC
PE/Dazzle™ 594 anti-mouse CX3CR1 SA011F11 FC
PE/Cyanine7 anti-mouse CX3CR1 SA011F11 FC
Biotin anti-mouse CX3CR1 SA011F11 FC,Cell Sep - Pos
Brilliant Violet 605™ anti-mouse CX3CR1 SA011F11 FC
Brilliant Violet 785™ anti-mouse CX3CR1 SA011F11 FC
Brilliant Violet 711™ anti-mouse CX3CR1 SA011F11 FC
Brilliant Violet 650™ anti-mouse CX3CR1 SA011F11 FC
Alexa Fluor® 700 anti-mouse CX3CR1 SA011F11 FC
APC/Fire™ 750 anti-mouse CX3CR1 SA011F11 FC
Pacific Blue™ anti-mouse CX3CR1 SA011F11 FC
TotalSeq™-A0563 anti-mouse CX3CR1 SA011F11 PG
TotalSeq™-C0563 anti-mouse CX3CR1 SA011F11 PG
TotalSeq™-B0563 anti-mouse CX3CR1 SA011F11 PG
APC/Cyanine7 anti-mouse CX3CR1 Antibody SA011F11 FC
PE/Cyanine5 anti-mouse CX3CR1 SA011F11 FC
PE/Fire™ 700 anti-mouse CX3CR1 SA011F11 FC
APC/Fire™ 810 anti-mouse CX3CR1 SA011F11 FC
PE/Fire™ 640 anti-mouse CX3CR1 SA011F11 FC
PE/Fire™ 810 anti-mouse CX3CR1 SA011F11 FC
PerCP/Fire™ 806 anti-mouse CX3CR1 SA011F11 FC
Brilliant Violet 570™ anti-mouse CX3CR1 SA011F11 FC
PerCP/Fire™ 780 anti-mouse CX3CR1 SA011F11 FC
Spark Red™ 718 anti-mouse CX3CR1 (Flexi-Fluor™) SA011F11 FC
Spark PLUS UV395™ anti-mouse CX3CR1 SA011F11 FC
Spark Blue™ 574 anti-mouse CX3CR1 (Flexi-Fluor™) SA011F11 FC
Spark Blue™ 550 anti-mouse CX3CR1 (Flexi-Fluor™) SA011F11 FC
Go To Top Version: 1    Revision Date: 09-06-2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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