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Ultra-LEAF™ Purified Mouse IgG2b, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
MPC-11 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
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Cat # Size Price Quantity Check Availability Save
400347 100 µg 88€
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400348 1 mg 120€
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400369 5 mg 432€
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400370 25 mg 1127€
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400377 50 mg 1884€
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400378 100 mg 2707€
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

The MPC-11 immunoglobulin has unknown specificity. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
ICFC, ICC, IHC, IP, WB, FA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.

Application Notes

Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA).

Application References
  1. Smed-Sörensen A, et al. 2008. Blood 111:5037. (FA) PubMed
  2. Podolin PL, et al. 2008. J. Immunol. 180:7989. (FC) PubMed
Product Citations
  1. Gu C, et al. 2021. Front Immunol. 12:678036. PubMed
  2. Hanna J, et al. 2022. Nat Commun. 13:4075. PubMed
  3. Guo X, et al. 2021. iScience. 24:103295. PubMed
RRID
AB_3097662 (BioLegend Cat. No. 400347)
AB_3097662 (BioLegend Cat. No. 400348)
AB_3097662 (BioLegend Cat. No. 400369)
AB_3097662 (BioLegend Cat. No. 400370)
AB_3097662 (BioLegend Cat. No. 400377)
AB_3097662 (BioLegend Cat. No. 400378)

Antigen Details

Gene ID
NA

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All Reagents Request Custom Conjugation
Description Clone Applications
APC Mouse IgG2b, κ Isotype Ctrl MPC-11 FC,ICFC
Biotin Mouse IgG2b, κ Isotype Ctrl MPC-11 FC,ICFC
FITC Mouse IgG2b, κ Isotype Ctrl MPC-11 FC,ICFC
PE Mouse IgG2b, κ Isotype Ctrl MPC-11 FC,ICFC
PE/Cyanine5 Mouse IgG2b, κ Isotype Ctrl MPC-11 FC,ICFC
Purified Mouse IgG2b, κ Isotype Ctrl MPC-11 FC,ICFC,ICC,IHC,IP,WB,FA,ChIP
APC/Cyanine7 Mouse IgG2b, κ Isotype Ctrl MPC-11 FC,ICFC
PE/Cyanine7 Mouse IgG2b, κ Isotype Ctrl MPC-11 FC,ICFC
Alexa Fluor® 647 Mouse IgG2b, κ Isotype Ctrl MPC-11 FC,ICFC
Alexa Fluor® 488 Mouse IgG2b, κ Isotype Ctrl MPC-11 FC,ICFC
Pacific Blue™ Mouse IgG2b, κ Isotype Ctrl MPC-11 FC,ICFC
Alexa Fluor® 700 Mouse IgG2b, κ Isotype Ctrl MPC-11 FC,ICFC
PerCP Mouse IgG2b, κ Isotype Ctrl MPC-11 FC,ICFC
PerCP/Cyanine5.5 Mouse IgG2b, κ Isotype Ctrl MPC-11 FC,ICFC
Brilliant Violet 421™ Mouse IgG2b, κ Isotype Ctrl MPC-11 FC,ICFC
Brilliant Violet 570™ Mouse IgG2b, κ Isotype Ctrl MPC-11 FC,ICFC
Brilliant Violet 510™ Mouse IgG2b, κ Isotype Ctrl MPC-11 FC,ICFC
Ultra-LEAF™ Purified Mouse IgG2b, κ Isotype Ctrl MPC-11 FC,ICFC,ICC,IHC,IP,WB,FA
Brilliant Violet 605™ Mouse IgG2b, κ Isotype Ctrl MPC-11 FC,ICFC
Brilliant Violet 650™ Mouse IgG2b, κ Isotype Ctrl MPC-11 FC,ICFC
Brilliant Violet 711™ Mouse IgG2b, κ Isotype Ctrl MPC-11 FC,ICFC
Brilliant Violet 785™ Mouse IgG2b, κ Isotype Ctrl MPC-11 FC,ICFC
PE/Dazzle™ 594 Mouse IgG2b, κ Isotype Ctrl MPC-11 FC
Alexa Fluor® 594 Mouse IgG2b, κ Isotype Ctrl MPC-11 ICC
APC/Fire™ 750 Mouse IgG2b, κ Isotype Ctrl MPC-11 FC,ICFC
GoInVivo™ Purified Mouse IgG2b, κ Isotype Ctrl MPC-11 FC,ICFC,ICC,IHC,IP,WB,FA
FITC Mouse IgG2b, κ Isotype Ctrl MPC-11 FC
Pacific Blue™ Mouse IgG2b, κ Isotype Ctrl MPC-11 FC
APC Mouse IgG2b, κ Isotype Ctrl MPC-11 FC
TotalSeq™-A0092 Mouse IgG2b, κ isotype Ctrl MPC-11 PG
PE/Cyanine7 Mouse IgG2b, κ Isotype Ctrl MPC-11 FC
TotalSeq™-B0092 Mouse IgG2b, κ isotype Ctrl MPC-11 PG
TotalSeq™-C0092 Mouse IgG2b, κ isotype Ctrl MPC-11 PG
PerCP/Cyanine5.5 Mouse IgG2b, κ Isotype Ctrl MPC-11 FC
TotalSeq™-D0092 Mouse IgG2b, κ Isotype Ctrl MPC-11 PG
GMP APC Mouse IgG2b, κ Isotype Ctrl MPC-11 FC
GMP FITC Mouse IgG2b, κ Isotype Ctrl MPC-11 FC
GMP Pacific Blue™ Mouse IgG2b, κ Isotype Ctrl MPC-11 FC
GMP PerCP/Cyanine5.5 Mouse IgG2b, κ Isotype Ctrl MPC-11 FC
Brilliant Violet 750™ Mouse IgG2b, κ Isotype Ctrl MPC-11 FC,ICFC
Spark NIR 685™ Mouse IgG2b, κ Isotype Ctrl MPC-11 FC
PE/Fire™ 810 Mouse IgG2b, κ Isotype Ctrl MPC-11 FC
TotalSeq™-Bn0092 Mouse IgG2b, κ Isotype Ctrl MPC-11 SB
PE/Fire™ 700 Mouse IgG2b, κ Isotype Ctrl MPC-11 FC
KIRAVIA Blue 520™ Mouse IgG2b, κ Isotype Ctrl MPC-11 FC
PE/Fire™ 640 Mouse IgG2b, κ Isotype Ctrl MPC-11 FC,ICFC
GMP PE/Cyanine7 Mouse IgG2b, κ Isotype Ctrl MPC-11 FC
APC/Fire™ 810 Mouse IgG2b, κ Isotype Ctrl MPC-11 FC,ICFC
Spark YG™ 570 Mouse IgG2b, κ Isotype Ctrl MPC-11 FC
PerCP/Fire™ 806 Mouse IgG2b, κ Isotype Ctrl MPC-11 FC
Spark Red™ 718 Mouse IgG2b, κ Isotype Ctrl MPC-11 FC,ICFC
Spark Blue™ 515 Mouse IgG2b, κ Isotype Ctrl MPC-11 FC,ICFC
PerCP/Fire™ 780 Mouse IgG2b, κ Isotype Ctrl MPC-11 FC
PE/Fire™ 744 Mouse IgG2b, κ Isotype Ctrl MPC-11 FC,ICFC
Spark PLUS UV395™ Mouse IgG2b, κ Isotype Ctrl MPC-11 FC,ICFC
TotalSeq™-B1473 Mouse IgG2b, κ Isotype Ctrl MPC-11 ICPG
Go To Top Version: 4    Revision Date: 08-31-2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC Mouse IgG2b, κ Isotype Ctrl

  • Biotin Mouse IgG2b, κ Isotype Ctrl

  • FITC Mouse IgG2b, κ Isotype Ctrl

  • PE Mouse IgG2b, κ Isotype Ctrl

  • PE/Cyanine5 Mouse IgG2b, κ Isotype Ctrl

  • Purified Mouse IgG2b, κ Isotype Ctrl

    MPC-11_ChIP_IgG2b-k_ctrl_1_060618_updated.png
    Chromatin Immunoprecipitations (ChIP) were performed with cr...
    MPC-11_ChIP_IgG2b-k_ctrl_2_060618_updated.png
    Chromatin Immunoprecipitations (ChIP) were performed with cr...

  • APC/Cyanine7 Mouse IgG2b, κ Isotype Ctrl

  • PE/Cyanine7 Mouse IgG2b, κ Isotype Ctrl

  • Alexa Fluor® 647 Mouse IgG2b, κ Isotype Ctrl

  • Alexa Fluor® 488 Mouse IgG2b, κ Isotype Ctrl

  • Pacific Blue™ Mouse IgG2b, κ Isotype Ctrl

  • Alexa Fluor® 700 Mouse IgG2b, κ Isotype Ctrl

  • PerCP Mouse IgG2b, κ Isotype Ctrl

    MPC-11_PerCP_122607
    Human peripheral blood lymphocytes stained with MPC-11 PerCP

  • PerCP/Cyanine5.5 Mouse IgG2b, κ Isotype Ctrl

    MPC-11_PerCPCy55_122607
    Human peripheral blood lymphocytes stained with MPC-11 PerCP...

  • Brilliant Violet 421™ Mouse IgG2b, κ Isotype Ctrl

  • Brilliant Violet 570™ Mouse IgG2b, κ Isotype Ctrl

  • Brilliant Violet 510™ Mouse IgG2b, κ Isotype Ctrl

  • Ultra-LEAF™ Purified Mouse IgG2b, κ Isotype Ctrl

  • Brilliant Violet 605™ Mouse IgG2b, κ Isotype Ctrl

  • Brilliant Violet 650™ Mouse IgG2b, κ Isotype Ctrl

  • Brilliant Violet 711™ Mouse IgG2b, κ Isotype Ctrl

  • Brilliant Violet 785™ Mouse IgG2b, κ Isotype Ctrl

  • PE/Dazzle™ 594 Mouse IgG2b, κ Isotype Ctrl

  • Alexa Fluor® 594 Mouse IgG2b, κ Isotype Ctrl

  • APC/Fire™ 750 Mouse IgG2b, κ Isotype Ctrl

  • GoInVivo™ Purified Mouse IgG2b, κ Isotype Ctrl

  • FITC Mouse IgG2b, κ Isotype Ctrl

  • Pacific Blue™ Mouse IgG2b, κ Isotype Ctrl

  • APC Mouse IgG2b, κ Isotype Ctrl

  • TotalSeq™-A0092 Mouse IgG2b, κ isotype Ctrl

  • PE/Cyanine7 Mouse IgG2b, κ Isotype Ctrl

  • TotalSeq™-B0092 Mouse IgG2b, κ isotype Ctrl

  • TotalSeq™-C0092 Mouse IgG2b, κ isotype Ctrl

  • PerCP/Cyanine5.5 Mouse IgG2b, κ Isotype Ctrl

  • TotalSeq™-D0092 Mouse IgG2b, κ Isotype Ctrl

  • GMP APC Mouse IgG2b, κ Isotype Ctrl

  • GMP FITC Mouse IgG2b, κ Isotype Ctrl

  • GMP Pacific Blue™ Mouse IgG2b, κ Isotype Ctrl

  • GMP PerCP/Cyanine5.5 Mouse IgG2b, κ Isotype Ctrl

  • Brilliant Violet 750™ Mouse IgG2b, κ Isotype Ctrl

  • Spark NIR 685™ Mouse IgG2b, κ Isotype Ctrl

  • PE/Fire™ 810 Mouse IgG2b, κ Isotype Ctrl

  • TotalSeq™-Bn0092 Mouse IgG2b, κ Isotype Ctrl

  • PE/Fire™ 700 Mouse IgG2b, κ Isotype Ctrl

  • KIRAVIA Blue 520™ Mouse IgG2b, κ Isotype Ctrl

  • PE/Fire™ 640 Mouse IgG2b, κ Isotype Ctrl

  • GMP PE/Cyanine7 Mouse IgG2b, κ Isotype Ctrl

  • APC/Fire™ 810 Mouse IgG2b, κ Isotype Ctrl

  • Spark YG™ 570 Mouse IgG2b, κ Isotype Ctrl

  • PerCP/Fire™ 806 Mouse IgG2b, κ Isotype Ctrl

  • Spark Red™ 718 Mouse IgG2b, κ Isotype Ctrl

  • Spark Blue™ 515 Mouse IgG2b, κ Isotype Ctrl

  • PerCP/Fire™ 780 Mouse IgG2b, κ Isotype Ctrl

  • PE/Fire™ 744 Mouse IgG2b, κ Isotype Ctrl

  • Spark PLUS UV395™ Mouse IgG2b, κ Isotype Ctrl

  • TotalSeq™-B1473 Mouse IgG2b, κ Isotype Ctrl

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