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APC anti-mouse CD117 (c-Kit) Antibody

Pricing & Availability
Clone
2B8 (See other available formats)
Regulatory Status
RUO
Other Names
c-KIT, Stem Cell Factor Receptor (SCFR)
Isotype
Rat IgG2b, κ
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Product Citations
publications
2B8_APC_1_072611
  • 2B8_APC_1_072611
  • 2B8_APC_2_072611
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105811 25 µg 68€
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105812 100 µg 168€
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Description

CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse bone marrow mast cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity.

Application References

(PubMed link indicates BioLegend citation)
  1. Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC)
  2. Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC)
  3. Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC)
  4. Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC)
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  2. Lengefeld J, et al. 2021. Sci Adv. 7:eabk0271. PubMed
  3. Julien E, et al. 2021. Stem Cells. 39:636. PubMed
  4. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  5. Roy IM, et al. 2022. iScience. 25:105171. PubMed
  6. Sohn J, et al. 2022. Blood Adv. 6:2557. PubMed
  7. Xin DQ, et al. 2022. Neural Regen Res. 17:2238. PubMed
  8. Yuan O, et al. 2022. Sci Adv. 8:eabm9987. PubMed
  9. Ramdas B, et al. 2022. Mol Ther. 30:2505. PubMed
  10. Jabbar S, et al. 2022. Exp Hematol Oncol. 11:83. PubMed
  11. Barkaway A, et al. 2021. Immunity. . PubMed
  12. Tran NT, et al. 2019. Cell Rep. 28:3510. PubMed
  13. Rahman SMT, et al. 2022. Cell Rep. 41:111682. PubMed
  14. Guan H, et al. 2023. Int J Mol Sci. 24:. PubMed
  15. Elbasiony E, et al. 2023. NPJ Aging. 9:2. PubMed
  16. Ma S, et al. 2023. Front Cell Dev Biol. 11:1140554. PubMed
  17. Wang X, et al. 2023. Nature. 618:808. PubMed
  18. Wu J, et al. 2022. Nat Commun. 13:7321. PubMed
  19. Davis FM, et al. 2020. J Immunol. 2503:204. PubMed
  20. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  21. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  22. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  23. Chen J, et al. 2021. Sci Adv. 7:. PubMed
  24. Gutiérrez-Gutiérrez Ó, et al. 2021. EMBO Rep. 22:e52905. PubMed
  25. Pessoa Rodrigues C, et al. 2021. Sci Adv. 7:. PubMed
  26. Sasse C, et al. 2022. Pathogens. 11:. PubMed
  27. Chen CC, et al. 2020. Cancer Cell. 37:71. PubMed
  28. Yu K, et al. 2021. Cell Reports. 35(12):109273. PubMed
  29. Zhu H, et al. 2018. Elife. 7:. PubMed
  30. Al-Rifai R, et al. 2022. Nat Commun. 13:6592. PubMed
  31. Ludin A, et al. 2012. Nat Immunol. 13:1072. PubMed
  32. Sun Y et al. 2018. Cancer cell. 34(4):643-658 . PubMed
  33. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  34. Guo B, et al. 2018. Nat Med. 24:360. PubMed
  35. Gao B, et al. 2018. Aging Cell. 3. PubMed
  36. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  37. Davis FM, et al. 2019. Arterioscler Thromb Vasc Biol. 39:2353. PubMed
  38. Markov SD, et al. 2021. Mol Cancer Ther. 20:2457. PubMed
  39. Beauchamp EM, et al. 2021. Blood Cancer Discov. 6:. PubMed
  40. Nguyen DH, et al. 2020. Nat Cell Biol. 22:1423. PubMed
  41. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  42. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  43. Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed
  44. Wang L, et al. 2021. Cancer Immunol Res. 9:348. PubMed
  45. Widner DB, et al. 2021. J Cachexia Sarcopenia Muscle. 12:1079. PubMed
  46. Eisele AS, et al. 2022. Elife. 11:. PubMed
  47. Morshed M, et al. 2014. J Immunol. 192:5314. PubMed
  48. Koh F, et al. 2015. Proc Natl Acad Sci U S A. 112:1734. PubMed
  49. Ritter M, et al. 2020. Ann Hematol. 99:2329. PubMed
  50. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  51. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  52. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  53. Parada-Kusz M, et al. 2018. Dis Model Mech. 11:. PubMed
  54. Plein A, et al. 2018. Nature. 562:223. PubMed
  55. Trompette A, et al. 2022. Mucosal Immunol. :. PubMed
  56. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  57. Dong S, et al. 2021. Nature. 591:117. PubMed
  58. Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed
  59. Lu X, et al. 2016. Nat Commun. 7: 12719. PubMed
  60. Bahal R, et al. 2016. Nat Commun. 7:13304. PubMed
  61. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  62. Hu Q, et al. 2018. Nat Biomed Eng. 0.660416667. PubMed
  63. La Rose AM, et al. 2021. Mol Metab. 53:101265. PubMed
  64. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  65. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  66. Umemoto T, et al. 2008. Biochem Biophys Res Commun. 365:176. PubMed
  67. Convente MR, et al. 2018. J Bone Miner Res. 33:269. PubMed
  68. Oka Y, et al. 2020. Sci Adv. 6:. PubMed
  69. Zhu M, et al. 2015. J Immunol. 195: 4492 - 4502. PubMed
  70. Lebert-Ghali C, et al. 2016. Blood. 127: 87-90. PubMed
  71. Wang X, et al. 2020. Cell Res. 30:1109. PubMed
  72. Tran NT, et al. 2020. STAR Protocols. 1(1):100028. PubMed
  73. Sibon D et al. 2019. Cell reports. 26(12):3246-3256 . PubMed
  74. Tsuchiya K, et al. 2019. Nat Commun. 10:2091. PubMed
  75. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  76. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  77. Zhang Z, et al. 2021. Exp Dermatol. 30:102. PubMed
  78. Li W, et al. 2021. PLoS Pathog. 17:e1009462. PubMed
  79. Gandhapudi S, et al. 2015. J Immunol. 194:3820. PubMed
  80. Gao Y, et al. 2020. Immunity. 52(6):1007-1021.e8. PubMed
  81. Inverso D, et al. 2021. Developmental Cell. 56(11):1677-1693.e10. PubMed
  82. N. H, et al. 2017. Journal of Vascular Research. 10.1159/000464287. PubMed
  83. Ha TC, et al. 2020. Hum Gene Ther. 32:458. PubMed
  84. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  85. Cai Z, et al. 2020. Cell Rep. 31:107816. PubMed
  86. Legrand JMD, et al. 2019. Nat Commun. 10:2278. PubMed
  87. Engblom C, et al. 2017. Science. 358:6367. PubMed
  88. Suzuki S, et al. 2021. Cell Reports. 34(7):108752. PubMed
  89. Wilson ZS, et al. 2020. Sci Rep. 10:2142. PubMed
  90. Koide S, et al. 2022. iScience. 25:103603. PubMed
  91. Zhuo X, et al. 2021. Biomed Rep. 15:64. PubMed
  92. Yokomizo-Nakano T, et al. 2020. Cancer Res. 80:2523. PubMed
  93. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
  94. Christoforou N, et al. 2013. PLoS One. 8:65963. PubMed
  95. Artinger E, et al. 2013. Proc Natl Acad Sci U S A. 110:12000. PubMed
  96. Wang E, et al. 2013. Proc Natl Acad Sci U S A. 110:3901. PubMed
  97. Sabnis A, et al. 2009. PLoS One. 7:e59. PubMed
  98. He M, et al. 2020. Cell Metabolism. 31(3):580-591. PubMed
  99. Dhayade S, et al. 2020. Nutrients. 12:. PubMed
  100. Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
  101. Golan K et al. 2018. Cell stem cell. 23(4):572-585 . PubMed
  102. Brian BF 4th et al. 2019. Elife. 8 pii: e46043. PubMed
  103. Matsuzaki Y, et al. 2015. Biomed Rep. 1: 91 - 97. PubMed
  104. Li K, et al. 2020. Nat Commun. 0.795138889. PubMed
  105. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  106. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
  107. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  108. Kastenschmidt JM, et al. 2021. Cell Rep. 35:108997. PubMed
  109. Collins C, et al. 2014. Proc Natl Acad Sci U S A. 111:9899. PubMed
  110. Li ZG, et al. 2020. Chin Med J (Engl). 1943:133. PubMed
  111. Gozdecka M, et al. 2018. Nat Genet. 50:883. PubMed
  112. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  113. Liu J, et al. 2012. Proc Natl Acad Sci U S A. 109:10018. PubMed
  114. Cui Y, et al. 2020. Eur J Pharmacol. 881:173233. PubMed
  115. Gu Z, et al. 2021. Nat Genet. 53:672. PubMed
  116. Lei F, et al. 2012. J Vis Exp. 63: 3986. PubMed
  117. Pessoa Rodrigues C, et al. 2020. Sci Adv. 6:eaaz4815. PubMed
RRID
AB_313220 (BioLegend Cat. No. 105811)
AB_313220 (BioLegend Cat. No. 105812)

Antigen Details

Structure
Ig superfamily, 145 kD
Distribution

Hematopoietic stem cells, AML, mast cells

Function
Growth factor receptor, tyrosine kinase
Ligand/Receptor
Stem Cell Factor (SCF)
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5.

Gene ID
16590 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD117
Specificity Alt (DOES NOT SHOW ON TDS):
CD117
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD117 on UniProt.org

Other Formats

View All CD117 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD117 (c-Kit) 2B8 FC
Biotin anti-mouse CD117 (c-Kit) 2B8 FC
FITC anti-mouse CD117 (c-Kit) 2B8 FC
PE anti-mouse CD117 (c-Kit) 2B8 FC
PE/Cyanine5 anti-mouse CD117 (c-Kit) 2B8 FC
Purified anti-mouse CD117 (c-Kit) 2B8 FC,IHC-F
PE/Cyanine7 anti-mouse CD117 (c-Kit) 2B8 FC
Alexa Fluor® 488 anti-mouse CD117 (c-Kit) 2B8 FC,IHC-F
Alexa Fluor® 647 anti-mouse CD117 (c-Kit) 2B8 FC,IHC-F
Pacific Blue™ anti-mouse CD117 (c-Kit) 2B8 FC
PerCP/Cyanine5.5 anti-mouse CD117 (c-kit) 2B8 FC
PerCP anti-mouse CD117 (c-kit) 2B8 FC
APC/Cyanine7 anti-mouse CD117 (c-kit) 2B8 FC
Brilliant Violet 421™ anti-mouse CD117 (c-Kit) 2B8 FC,SB
Purified anti-mouse CD117 (c-Kit) (Maxpar® Ready) 2B8 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD117 (c-Kit) 2B8 FC
Brilliant Violet 711™ anti-mouse CD117 (c-Kit) 2B8 FC
Alexa Fluor® 594 anti-mouse CD117 (c-Kit) 2B8 IHC-F,3D IHC
APC/Fire™ 750 anti-mouse CD117 (c-Kit) 2B8 FC
Brilliant Violet 510™ anti-mouse CD117 (c-Kit) 2B8 FC
Brilliant Violet 785™ anti-mouse CD117 (c-Kit) 2B8 FC
TotalSeq™-A0012 anti-mouse CD117 (c-kit) 2B8 PG
Brilliant Violet 605™ anti-mouse CD117 (c-Kit) 2B8 FC
Alexa Fluor® 700 anti-mouse CD117 (c-Kit) 2B8 FC
TotalSeq™-B0012 anti-mouse CD117 (c-Kit) 2B8 PG
TotalSeq™-C0012 anti-mouse CD117 (c-Kit) 2B8 PG
Spark NIR™ 685 anti-mouse CD117 (c-kit) 2B8 FC
Brilliant Violet 650™ anti-mouse CD117 (c-kit) 2B8 FC
Spark Red™ 718 anti-mouse CD117 (c-kit) (Flexi-Fluor™) 2B8 FC
Spark Blue™ 574 anti-mouse CD117 (c-kit) (Flexi-Fluor™) 2B8 FC
Spark Blue™ 550 anti-mouse CD117 (c-kit) (Flexi-Fluor™) 2B8 FC
Go To Top Version: 2    Revision Date: 06.28.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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