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Alexa Fluor® 647 anti-mouse CD11c Antibody

Pricing & Availability
Clone
N418 (See other available formats)
Regulatory Status
RUO
Other Names
αX integrin, integrin αX chain, CR4, p150, ITGAX
Isotype
Armenian Hamster IgG
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Product Citations
publications
1_N418_A647_CD11c_Antibody_1_HR_090717
C57BL/6 mouse splenocytes stained with N418 Alexa Fluor® 647
  • 1_N418_A647_CD11c_Antibody_1_HR_090717
    C57BL/6 mouse splenocytes stained with N418 Alexa Fluor® 647
  • 2_N418_AF647_CD11c_Antibody_7_3D-IHC_092121.png
    Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen section was processed according to the Ce3DTM Tissue Clearing Kit protocol (cat. no. 427701). The section was costained with anti-mouse/human CD45R/B220 Antibody (clone RA3-6B2) Alexa Fluor® 488 at 5 µg/mL (green), anti-mouse CD8a Antibody (clone 53-6.7) Alexa Fluor® 594 at 5 µg/mL (yellow), and anti-mouse CD11c Antibody (clone N418) Alexa Fluor® 647 at 5 µg/mL (magenta). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
    Watch the video.
  • 3_N418_A647_CD11c_Antibody_3_HR_090717
    Fixed whole mount mouse epidermis Langerhans cells were stained with Alexa Fluor® 647 CD11c (red) (clone N418) and PE CD207 (yellow) (clone 4C7). Isotype controls at the same concentrations were used for the negative control. Cells were mounted in Prolong Gold and imaged with a Leica SP8 confocal. Image courtesy of Grzegorz Chodaczek and Zbigniew Mikulski at LIAI.
  • 4_N418_A647_CD11c_Antibody_4_HR_090717
    Live intravital mouse spleen imaging. PE CD11b (red) (clone M1/70), Alexa Fluor® 488 F4/80 (green) (clone BM8), and Alexa Fluor® 647 CD11c (blue) (clone N418) were imaged 30 minutes after iv injection of 10 µg per antibody. Isotype controls at the same concentrations, time post injection, and exposure conditions were used for the negative control. Cells were imaged with a Leica SP5 confocal on anesthetized mice. Image courtesy of Grzegorz Chodaczek and Zbigniew Mikulski at LIAI.
  • 5_N418_A647_CD11c_Antibody_6_HR_090717
    Fixed whole mount mouse spleen was stained with FITC CD172a (red) (clone P84), Alexa Fluor® CD11c (yellow) (clone N418), and PE CD11b (blue) (clone M1/70). Isotype controls at the same concentrations were used for the negative control. Cells were mounted in Prolong Gold and imaged with a Leica SP8 confocal. Image courtesy of Grzegorz Chodaczek and Zbigniew Mikulski at LIAI.
  • 6_36_Mouse_Spleen_CD11c_CD68
    Confocal image of C57BL/6 mouse spleen sample acquired using the IBEX method of highly multiplexed antibody-based imaging: CD11c (cyan) in Cycle 2 and CD68 (red) in Cycle 3. Tissues were prepared using ~1% (vol/vol) formaldehyde and a detergent. Following fixation, samples are immersed in 30% (wt/vol) sucrose for cryoprotection. Images are courtesy of Drs. Andrea J. Radtke and Ronald N. Germain of the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
Compare all formats See Alexa Fluor® 647 spectral data See high resolution IBEX data...
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117314 25 µg 81€
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117312 100 µg 184€
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Description

CD11c is a 150 kD glycoprotein also known as αX integrin, CR4, and p150. CD11c forms a αXβ2 heterodimer with β2 integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. The αXβ2 integrin plays an important role in cell-cell contact by binding its ligands: iC3b, fibrinogen, and CD54.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse spleen dendritic cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
3D IHC - Verified
IHC - Reported but collaborator, not verified in house
ICC, SB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µL volume. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for other applications.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, immunofluorescence microscopy5, 9 (Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10), and spatial biology (IBEX)22,23.

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References

(PubMed link indicates BioLegend citation)
  1. Granucci F, et al. 1997. J. Immunol. 159:1794.
  2. Stokes RW, et al. 1998. J. Immunol. 160:5514.
  3. Metlay JP, et al. 1990. J. Exp. Med. 171:1753. (IHC, IP)
  4. Ma XT, et al. 2006. Cancer Research 66:1169.
  5. Chin RK, et al. 2006. J. Immunol. 177:290. (IF)
  6. Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC) PubMed
  7. Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) PubMed
  8. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
  9. You Y, et al. 2009. J. Immunol. 182:7343. (IF) PubMed
  10. Roland CL, et al. 2009. Mol. Cancer Res. 8:1761. (IHC, FC) PubMed
  11. Wikstrom M, et al.2006. J. Immunol. 177:913. PubMed
  12. Pericolini E, et al. 2008. J. Leukocyte Biol. 83:1286. PubMed
  13. Randall LM, et al. 2008. Infect. Immun.76:3312. PubMed
  14. Fahlen-Yrild L, et al. 2009. J. Immunol. 183:5032. PubMed
  15. Osterholzer JJ, et al. 2009. J. Immunol. 183:8044. PubMed
  16. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  17. Eisenach PA, et al. 2010. J Cell Sci. 123:4182. PubMed
  18. Leppin K, et al. 2014. Invest. Ophthalmol. Vis. Sci. 55:3603. PubMed
  19. Sakai F, et al. 2014. PLoS One. 9:105370. PubMed
  20. Gibbins JD, et al. 2014. Blood. 124:2953. PubMed
  21. White CE, et al. 2015. J Immunol. 194:697. PubMed
  22. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  23. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  24. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Vella JL, et al. 2021. Life Sci Alliance. 4:. PubMed
  2. Kumpunya S, et al. 2023. Front Immunol. 13:1010764. PubMed
  3. Pernet E, et al. 2023. Nature. 614:530. PubMed
  4. Yeh CH, et al. 2022. Immunity. 55:272. PubMed
  5. Kim S, et al. 2020. Immunity. 53(4):759-774.e9. PubMed
  6. Reboldi A, et al. 2016. Sci Transl Med. 352: aaf4822. PubMed
  7. Lei G, et al. 2016. Antimicrob Agents Chemother. 60: 1289 - 1297. PubMed
  8. Groza D, et al. 2018. Oncoimmunology. 7:e1424676. PubMed
  9. Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed
  10. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  11. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  12. Bradford BM, et al. 2018. Parasite Immunol. 40:e12566. PubMed
  13. Castellanos CA, et al. 2021. Sci Immunol. 6:eabh0707. PubMed
  14. Roufaiel M, et al. 2016. Nat Immunol. 10.1038/ni.3564. PubMed
  15. Bates J, et al. 2009. J Immunol. 182:7539. PubMed
  16. Hays C, et al. 2020. eLife. 8:e48772.. PubMed
  17. Shen H, et al. 2022. Nat Commun. 13:6030. PubMed
  18. Haugh KA, et al. 2021. eLife. 0.416666666666667. PubMed
  19. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  20. Caronni N, et al. 2018. Cancer Res. 78:1685. PubMed
  21. Hagan AS, et al. 2020. Development. 147:00:00. PubMed
  22. An S, et al. 2019. Small. 15:e1805182. PubMed
  23. Hildebrand KM, et al. 2021. PLoS One. 16:e0253864. PubMed
  24. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  25. Kishore M et al. 2017. Immunity. 47(5):875-889 . PubMed
  26. Kim SH, et al. 2021. Cell Reports. 35(2):108995. PubMed
  27. Zheng D, et al. 2022. Acta Pharm Sin B. 12:2740. PubMed
  28. Koikawa K, et al. 2021. Cell. 184:4753. PubMed
  29. Collins N, et al. 2016. Nat Commun. 7:11514. PubMed
  30. Stevenson ER, et al. 2022. J Pharmacol Exp Ther. 382:356. PubMed
  31. Mambres D, et al. 2016. J Immunol. 196: 3780 - 3793. PubMed
  32. Vacca F, et al. 2020. eLife. 9:e54017.. PubMed
  33. Barbet G, et al. 2018. Immunity. 48:584. PubMed
  34. Shaikh H, et al. 2021. Front Immunol. 12:689896. PubMed
  35. Lindhorst A, et al. 2021. Cell Death Dis. 12:579. PubMed
  36. Chen S, et al. 2015. Blood. 126: 103 - 112. PubMed
  37. Tran NL, et al. 2022. Elife. 11:. PubMed
  38. Baker GJ, et al. 2020. Cell Syst. 0.647222222. PubMed
  39. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  40. Markey KA et al. 2019. Current protocols in immunology. 125(1):e72 . PubMed
  41. Fox B, et al. 2016. PLoS One. 12: 1006189. PubMed
  42. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  43. Song W, et al. 2022. Immunity. 55:290. PubMed
RRID
AB_389328 (BioLegend Cat. No. 117314)
AB_389328 (BioLegend Cat. No. 117312)

Antigen Details

Structure
Integrin α-chain, associates with integrin β2 (CD18), 150 kD
Distribution

Dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells

Function
Cellular adhesion
Ligand/Receptor
iC3b, fibrinogen
Cell Type
Dendritic cells, Epithelial cells, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lopez-Rodriguez C, et al. 1996. J. Immunol. 156:3780.

Gene ID
16411 View all products for this Gene ID
UniProt
View information about CD11c on UniProt.org

Other Formats

View All CD11c Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD11c N418 FC
Biotin anti-mouse CD11c N418 FC,IHC-F
FITC anti-mouse CD11c N418 FC
PE anti-mouse CD11c N418 FC
Purified anti-mouse CD11c N418 FC,CyTOF®,IP,IHC
Alexa Fluor® 488 anti-mouse CD11c N418 FC
Alexa Fluor® 647 anti-mouse CD11c N418 FC,IHC,ICC,3D IHC
PE/Cyanine5 anti-mouse CD11c N418 FC
PE/Cyanine7 anti-mouse CD11c N418 FC
Brilliant Violet 605™ anti-mouse CD11c N418 FC
Alexa Fluor® 700 anti-mouse CD11c N418 FC
Pacific Blue™ anti-mouse CD11c N418 FC
APC/Cyanine7 anti-mouse CD11c N418 FC
PerCP/Cyanine5.5 anti-mouse CD11c N418 FC
PerCP anti-mouse CD11c N418 FC
Brilliant Violet 421™ anti-mouse CD11c N418 FC,IHC
Brilliant Violet 570™ anti-mouse CD11c N418 FC
Brilliant Violet 785™ anti-mouse CD11c N418 FC
Brilliant Violet 510™ anti-mouse CD11c N418 FC
Brilliant Violet 650™ anti-mouse CD11c N418 FC
Purified anti-mouse CD11c (Maxpar® Ready) N418 FC,CyTOF®
Alexa Fluor® 594 anti-mouse CD11c N418 IHC-F,3D IHC
PE/Dazzle™ 594 anti-mouse CD11c N418 FC
Brilliant Violet 711™ anti-mouse CD11c N418 FC
APC/Fire™ 750 anti-mouse CD11c N418 FC
TotalSeq™-A0106 anti-mouse CD11c N418 PG
Brilliant Violet 750™ anti-mouse CD11c N418 FC
TotalSeq™-B0106 anti-mouse CD11c N418 PG
TotalSeq™-C0106 anti-mouse CD11c N418 PG
KIRAVIA Blue 520™ anti-mouse CD11c N418 FC
Spark Blue™ 550 anti-mouse CD11c N418 FC
Spark NIR™ 685 anti-mouse CD11c N418 FC
Spark UV™ 387 anti-mouse CD11c N418 FC
Spark Red™ 718 anti-mouse CD11c N418 FC
Spark Blue™ 515 anti-mouse CD11c N418 FC
PerCP/Fire™ 806 anti-mouse CD11c N418 FC
Spark PLUS UV395™ anti-mouse CD11c N418 FC
Spark Blue™ 574 anti-mouse CD11c (Flexi-Fluor™) N418 FC
Go To Top Version: 6    Revision Date: 04.28.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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