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PE/Cyanine7 anti-human CD4 Antibody

Pricing & Availability
Clone
OKT4 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
T4
Isotype
Mouse IgG2b, κ
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Product Citations
publications
OKT4_PECy7_060507
Human peripheral blood lymphocytes stained with OKT4 PE/Cyanine7
  • OKT4_PECy7_060507
    Human peripheral blood lymphocytes stained with OKT4 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
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317413 25 tests 123€
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317414 100 tests 249€
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Description

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human peripheral T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding. Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317453 and 317454).

In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.9

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061.
  3. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  4. Cicin-Sain L, et al. 2010. J. Immunol. 184:6739. PubMed
  5. Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36.
  6. Linder J, et al. 1987. Am. J. Pathol. 127:1.
  7. Boche D, et al. 1999. J. Neurovirol. 5:232. (IHC)
  8. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. USA. 76:4061. (Immunogen)
  9. Lederman S, et al. 1991. Mol Immunol. 28:1171-81.
Product Citations
  1. Shang P, et al. 2022. Acta Biochim Biophys Sin (Shanghai). 54:1497. PubMed
  2. Li M, et al. 2023. Front Immunol. 14:1087923. PubMed
  3. Segaliny AI, et al. 2023. Commun Biol. 6:380. PubMed
  4. Toriyama M, et al. 2023. Front Mol Biosci. 10:1149828. PubMed
  5. Ataya M, et al. 2020. Am J Transplant. 20:663. PubMed
  6. Bhatt RS, et al. 2021. Cancer Immunol Res. 9:156. PubMed
  7. Kwee BJ, et al. 2021. Proc Natl Acad Sci U S A. 118: . PubMed
  8. Zhou Y, et al. 2021. Methods Mol Biol. 2388:35. PubMed
  9. Alam A, et al. 2022. Cancer Cell. 40:153. PubMed
  10. Asashima H, et al. 2023. Cell Rep. 42:111895. PubMed
  11. Spitsin S, et al. 2020. Mol Ther Methods Clin Dev. 17:1088. PubMed
  12. Swadling L, et al. 2020. Cell Rep. 30:687. PubMed
  13. Hinterbrandner M, et al. 2021. JCI Insight. 6:e151797. PubMed
  14. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  15. Lieberman L, et al. 2016. Neurology. 86: 375 - 381. PubMed
  16. Kubo M, et al. 2018. Oncol Rep. 39:417. PubMed
  17. Song C, et al. 2022. Stem Cell Res Ther. 13:48. PubMed
  18. Bellini N, et al. 2022. iScience. 25:105234. PubMed
  19. Soriano-Sarabia N, et al. 2012. J Immunol. 189:5212. PubMed
  20. Konadu K, et al. 2015. J Infect Dis. 211:1712. PubMed
  21. Della–Torre E, et al. 2018. Arthritis Rheumatol. 70:1133. PubMed
  22. Ma YV, et al. 2021. MAbs. 13:2003281. PubMed
  23. Alkasalias T, et al. 2022. Cell Death Discov. 8:464. PubMed
  24. Wang A, et al. 2020. Front Immunol. 1.213194444. PubMed
  25. Lu Y, et al. 2021. Gastroenterology. 161:575. PubMed
  26. Court AC, et al. 2020. EMBO Rep. 21:e48052. PubMed
  27. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  28. Ellis-Connell AL, et al. 2018. J Virol. 92:e01748. PubMed
  29. Zhu Y, et al. 2019. Cell Stem Cell. 25:542. PubMed
  30. Morimoto Y, et al. 2018. Immunity. 49:134. PubMed
  31. Li YR, et al. 2021. Cell Rep Med. 2:100449. PubMed
  32. Blom L, et al. 2011. PLoS One. 6:e21695. PubMed
  33. Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
  34. Yang B, et al. 2015. Cell Immunol. Available online 12 August 2015. PubMed
  35. Podaza E, et al. 2020. Front Immunol. 11:1147. PubMed
  36. Biswas S, et al. 2022. Nat Commun. 13:2995. PubMed
  37. Coiras M, et al. 2016. Cell Rep. 14:2100-2107. PubMed
  38. Keskin DB, et al. 2019. Nature. 565:234. PubMed
  39. Kuranda K, et al. 2018. J Clin Invest. 128:5267. PubMed
RRID
AB_571958 (BioLegend Cat. No. 317413)
AB_571958 (BioLegend Cat. No. 317414)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
Go To Top Version: 4    Revision Date: 07.13.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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